Fingerprinting of Microorganisms by Protein and Lipopolysaccharide SDS-PAGE

Dijkshoorn, Lenie

doi:10.1016/b978-044450740-2/50005-8

Cited by 1 publication

(1 citation statement)

References 51 publications

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“…Whole-cell protein profile analysis by SDS-PAGE is generally considered a suitable identification method, providing reliable results that correlate well with DNA-DNA hybridization experiments (Pot et al 1994;Dijkshoorn 2001). This method was used in our study to confirm the species identification results obtained with the (GTG) 5 -PCR fingerprinting and biotyping.…”

Section: Resultsmentioning

confidence: 78%

Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

Švec

Nováková

Žáčková

et al. 2008

Antonie van Leeuwenhoek

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Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)(5) primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)(5)-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)(5) primer to be a fast and reliable method for identification of S. mutans.

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Section: Resultsmentioning

confidence: 78%