Fingerprinting of morphine using chromatographic purity profiling and multivariate data analysis

Acevska, Jelena; Stefkov, Gjoshe; Cvetkovikj, Ivana; Petkovska, Rumenka; Kulevanova, S.; Cho, JungHwan; Dimitrovska, Aneta

doi:10.1016/j.jpba.2015.02.016

Cited by 21 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 That part of the research proved the applicability of the method and highlighted its advantages over the existing pharmacopoeial HPLC method for determination of related substances of morphine, 10 regarding authentication and prevention of falsification.…”

Section: Methodsmentioning

confidence: 88%

Impurity profiling of morphine by liquid chromatography-heated electrospray ionization mass spectrometry (LC-HESI-MS)

Petkovska¹,

Acevska²,

Chachorovska³

et al. 2017

Turk J Chem

Self Cite

View full text Add to dashboard Cite

Separation of morphine and its impurities (related substances specified in relevant European Pharmacopoeia monographs, as well as the other naturally occurring coextracted alkaloids) was obtained within a close retention window on a reverse phase HPLC column, eluted with optimized gradient, consisting of methanol and 5 mM ammonium formate, adjusted to pH 10.2. Systematic optimization of the chromatographic conditions was carried out using design of experiments. According to their mass spectra, known and specified impurities were identified and tentative structures for unknown impurities were proposed. MS analyses were performed in positive ionization mode using heated electrospray ionization. Fragmentation patterns of the eluted compounds under optimized mass spectrometry conditions indicated that all detected impurities are structurally related to morphine.

show abstract

Section: Methodsmentioning

confidence: 88%

Impurity profiling of morphine by liquid chromatography-heated electrospray ionization mass spectrometry (LC-HESI-MS)

Petkovska¹,

Acevska²,

Chachorovska³

et al. 2017

Turk J Chem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Then, 13 batches of samples were processed by optimized method and 16 ingredients including 12 flavonoids such as baohuoside II, baohuoside I, epimedin A, epimedin B, epimedin C, quercitrin, sagittatoside A, sagittatoside B, hyperoside, icariside I, icariin and astragalin, 3 phenolic acids such as chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and 1 alkaloid such as magnoflorine were simultaneously evaluated by LC–MS/MS. The present assay was further applied to investigate and compare the main components of crude and processed Epimedium using multivariate data analysis (partial least squares discriminant analysis (PLS-DA)) [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species

et al. 2018

View full text Add to dashboard Cite

Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography–mass spectrometry (LC–MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare different batches of Epimedium based on the LC–MS/MS data. As a conclusion, the herbs collected from 6 areas were ascribed to 5 species by microscopic and appearance features. Meanwhile, all of the raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 16 analyzed compounds. The comparison results indicate that processing and species both have important influences on Epimedium compositions contents.

show abstract

“…Because substantial differences may exist in the quality and quantity of chemical components, detecting only one or two “effective” compounds is insufficient for effective quality control. Now, fingerprinting is comprehensively used to analyze food, herbs, as well as their preparations in various fields world widely [24,25,26]. Chromatographic fingerprint technology is currently used to comprehensively and qualitatively analyze the complex components of TCM.…”

Section: Introductionmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Compounds in Xiaoyan Lidan Tablets: Fingerprint and Quantitative Analysis Using UPLC-MS

et al. 2016

View full text Add to dashboard Cite

XiaoyanLidan tablets (XYLDTs) are traditional Chinese medicines frequently used for syndromes of the liver and gallbladder, cholecystitis and cholangitis. To evaluate the consistency of the quality of commercial XYLDT preparations, we established a simple and reliable ultra-performance liquid chromatography (UPLC) method with a photodiode array (PDA) detector and mass spectrometry (MS), including a fingerprint analysis and quantification of the main pharmacologically-active markers. In the UPLC-PDA detection-based fingerprint analysis of XYLDTs, approximately 39 peaks were found in the XYLDT chromatogram, 26 of which were attributed to Picrasmaquassioides, nine to Andrographis and four to Isodonserra. Subsequently, the structures of these bioactive markers were identified through ESI-MS analyses. Using the chemometricmethods of similarity analysis and principal component analysis, the five significant herbal componentswere determined as 4-methoxy-5-hydroxycanthin-6-one, andrographolide, dehydroandrographolide, neoandrographolide and rosmarinic acid, and these components were qualitatively assessed. Our experimental results demonstrated that combining the fingerprint analysis with UPLC-MS and multi-ingredient determination is useful for rapid pharmaceutical quality evaluation. Moreover, the combined approach can potentially differentiate the origin, determine the authenticity and assess the overall quality of the formulae.

show abstract

Fingerprinting of morphine using chromatographic purity profiling and multivariate data analysis

Cited by 21 publications

References 22 publications

Impurity profiling of morphine by liquid chromatography-heated electrospray ionization mass spectrometry (LC-HESI-MS)

Impurity profiling of morphine by liquid chromatography-heated electrospray ionization mass spectrometry (LC-HESI-MS)

Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species

Quality Evaluation of Traditional Chinese Medicine Compounds in Xiaoyan Lidan Tablets: Fingerprint and Quantitative Analysis Using UPLC-MS

Contact Info

Product

Resources

About