Fingerprints of resistant <i>Escherichia coli</i> O157:H7 from vegetables and environmental samples

Abakpa, Grace Onyukwo; Umoh, V. J.; Sijam, Kamaruzaman; Ibekwe, Mark

doi:10.1002/jsfa.8441

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…In the edible part of corn, E. coli was not isolated, and the bla and uidA genes were not detected. Although crops could be contaminated by pathogenic E. coli suspected to be livestock-associated from soil [32,33], the phyllosphere, endosphere, and soil microbiota were quite different [34,35], and the degree of bacterial transmission and persistence from soil to crops would vary according to the type of vegetable [36]. Another study showed that ARGs were transmitted into plants mainly through endophytic bacteria [37].…”

Section: Plos Onementioning

confidence: 99%

Low-frequency transmission and persistence of antimicrobial-resistant bacteria and genes from livestock to agricultural soil and crops through compost application

Fukuda,

Suzuki,

Makita

et al. 2024

PLoS ONE

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Livestock excrement is composted and applied to agricultural soils. If composts contain antimicrobial-resistant bacteria (ARB), they may spread to the soil and contaminate cultivated crops. Therefore, we investigated the degree of transmission of ARB and related antimicrobial resistance genes (ARGs) and, as well as clonal transmission of ARB from livestock to soil and crops through composting. This study was conducted at Rakuno Gakuen University farm in Hokkaido, Japan. Samples of cattle feces, solid and liquid composts, agricultural soil, and crops were collected. The abundance of Escherichia coli, coliforms, β-lactam-resistant E. coli, and β-lactam-resistant coliforms, as well as the copy numbers of ARG (specifically the bla gene related to β-lactam-resistant bacteria), were assessed using qPCR through colony counts on CHROMagar ECC with or without ampicillin, respectively, 160 days after compost application. After the application of the compost to the soil, there was an initial increase in E. coli and coliform numbers, followed by a subsequent decrease over time. This trend was also observed in the copy numbers of the bla gene. In the soil, 5.0 CFU g-1 E. coli was detected on day 0 (the day post-compost application), and then, E. coli was not quantified on 60 days post-application. Through phylogenetic analysis involving single nucleotide polymorphisms (SNPs) and using whole-genome sequencing, it was discovered that clonal blaCTX-M-positive E. coli and blaTEM-positive Escherichia fergusonii were present in cattle feces, liquid compost, and soil on day 0 as well as 7 days post-application. This showed that livestock-derived ARB were transmitted from compost to soil and persisted for at least 7 days in soil. These findings indicate a potential low-level transmission of livestock-associated bacteria to agricultural soil through composts was observed at low frequency, dissemination was detected. Therefore, decreasing ARB abundance during composting is important for public health.

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Section: Plos Onementioning

confidence: 99%

Low-frequency transmission and persistence of antimicrobial-resistant bacteria and genes from livestock to agricultural soil and crops through compost application

Fukuda,

Suzuki,

Makita

et al. 2024

PLoS ONE

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“…Previously, fingerprints of resistance Escherichia coli O157:H7 from vegetables and environmental samples from wastewater reuse in irrigation in Nigeria were reported 8 . Sustainable wastewater reuse should prevent both types of effects, requiring a holistic and integrated risk assessment 9 .…”

Section: Introductionmentioning

confidence: 99%

Wastewater Treatment Plants; Hot Spots of Emerging Contaminants in the Environment

Abakpa,

Wei,

Obioh

et al. 2023

Res. J. Microbiol

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Background and Objective:The Wupa wastewater treatment plant is a major sewage treatment plant in Abuja, Nigeria's capital. While, the Shahe Reservoir treatment plant is a major treatment plant in Beijing, China's capital. In this study, the quantification of selected antibiotics, the occurrence of antibiotic resistance genes and physicochemical parameters were assayed for both study areas. Materials and Methods: Samples from both treatment plants were collected for physicochemical and molecular studies. Samples were analyzed for total nitrogen (TN), total phosphorus (TP), chemical oxygen demand (COD) and total chemical oxygen demand (TCOD). Antibiotic residues were detected by the Solid Phase Extraction and Liquid Chromatography Method (SPE-LM). While antibiotics resistance genes were determined by Polymerase Chain Reaction (PCR). Results: About seventeen different antibiotics were detected with Ciprofloxacin having the highest concentration. Antibiotic resistance genes, Beta-lactam (bla OXA-1 ), Sulfonamide resistance genes (sul1 and sul2) and mobile genetic element, Class1 integron (Int1) were amplified from both study areas. Conclusion: Antibiotics and antibiotic resistance genes were confirmed in the study. The intl-1 gene, amplified for samples from both study areas may facilitate the proliferation and propagation of some antibiotic-resistant genes ARGs in this environment and represents public health risks. The need for control of potential ARG contamination is recommended.

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Characterization of Diarrheagenic Escherichia coli and Salmonella enterica from Produce in the Chobe District of Botswana

Bywater,

Dintwe,

Alexander

et al. 2024

Journal of Food Protection

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Fingerprints of resistant Escherichia coli O157:H7 from vegetables and environmental samples

Cited by 7 publications

References 32 publications

Low-frequency transmission and persistence of antimicrobial-resistant bacteria and genes from livestock to agricultural soil and crops through compost application

Low-frequency transmission and persistence of antimicrobial-resistant bacteria and genes from livestock to agricultural soil and crops through compost application

Wastewater Treatment Plants; Hot Spots of Emerging Contaminants in the Environment

Characterization of Diarrheagenic Escherichia coli and Salmonella enterica from Produce in the Chobe District of Botswana

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