Fingerstick Glycosylated Hemoglobin, Plasma Protein, and Albumin

Rendell, Marc; Brannan, Carol; Nierenberg, Julia; Rasbold, Kathy; Hestorff, Tim

doi:10.2337/diacare.10.5.629

Cited by 20 publications

(15 citation statements)

References 5 publications

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“…Sample collection, handling, and storage. Blood can be obtained by venipuncture or by finger-stick capillary sampling (237,238 ). Blood tubes should contain the anticoagulant specified by the manufacturer of the Hb A 1c assay method (EDTA can be used unless the manufacturer specifies otherwise).…”

Section: Iron Deficiency Anemia Increases Test Results (232 )mentioning

confidence: 99%

Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus

et al. 2011

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BACKGROUND Multiple laboratory tests are used to diagnose and manage patients with diabetes mellitus. The quality of the scientific evidence supporting the use of these tests varies substantially. APPROACH An expert committee compiled evidence-based recommendations for the use of laboratory testing for patients with diabetes. A new system was developed to grade the overall quality of the evidence and the strength of the recommendations. Draft guidelines were posted on the Internet and presented at the 2007 Arnold O. Beckman Conference. The document was modified in response to oral and written comments, and a revised draft was posted in 2010 and again modified in response to written comments. The National Academy of Clinical Biochemistry and the Evidence Based Laboratory Medicine Committee of the AACC jointly reviewed the guidelines, which were accepted after revisions by the Professional Practice Committee and subsequently approved by the Executive Committee of the American Diabetes Association. CONTENT In addition to long-standing criteria based on measurement of plasma glucose, diabetes can be diagnosed by demonstrating increased blood hemoglobin A1c (Hb A1c) concentrations. Monitoring of glycemic control is performed by self-monitoring of plasma or blood glucose with meters and by laboratory analysis of Hb A1c. The potential roles of noninvasive glucose monitoring, genetic testing, and measurement of autoantibodies, urine albumin, insulin, proinsulin, C-peptide, and other analytes are addressed. SUMMARY The guidelines provide specific recommendations that are based on published data or derived from expert consensus. Several analytes have minimal clinical value at present, and their measurement is not recommended.

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Section: Iron Deficiency Anemia Increases Test Results (232 )mentioning

confidence: 99%

Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus

et al. 2011

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“…Whole blood glucose was measured using an Exactech meter. GHb was measured by an aminophenylboronic acid affinity procedure as we have previously described [9,10].…”

Section: Methodsmentioning

confidence: 99%

Decreased skin blood flow early in the course of streptozotocin-induced diabetes mellitus in the rat

et al. 1993

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We have previously used laser Doppler technology to demonstrate that skin blood flow is reduced in Type 1 (insulin-dependent) diabetic patients. The possibility of using the skin as an extremely accessible indicator of diabetic microvascular disease is attractive. The streptozotocin diabetic rat is an appealing potential animal model. We performed measurements of skin blood flow in two rat species, nine Sprague Dawley (SD) rats and nine Wistar Kyoto (WKY) rats, observing early changes following the inception of diabetes. Four of the SD rats and five of the WKY rats were made diabetic, the rest serving as controls. There were no significant differences in skin blood flow between the two rat strains. As in man, there appear to be rat skin sites with primarily nutritive capillary supply and those with arteriovenous anastomotic predominance. The back and base of tail, both hair-covered areas, demonstrated low flow characteristics, consistent with nutritive perfusion. In contrast, the plantar surface of the paw behaved similarly to the finger or toe pulps in man, sites of arteriovenous perfusion, with high basal flow and a marked increment with thermal stimulation. In diabetic rats of both species, there was significantly lower flow at the back and base of tail than in non-diabetic animals. The differences were of the order of 30-40%. As a function of time, the decrease in blood flow at the base of tail parallelled the increase in glycohaemoglobin levels in the diabetic rats. In contrast, blood flow at the plantar surface of the paw was unchanged throughout the 3-month post-streptozotocin observation period.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Protein determination after separation on affinity columns is accomplished by ultraviolet absorbance at 280 nm or Coomassie Blue reagent at 595 nm. Albumin in glycated and nonglycated fractions can be determined with bromcresol green or enzyme-linked immunosorbent assay (173).…”

Section: Glycated Serum Proteinsmentioning

confidence: 99%