Fingerstick Helicobacter Pylori Antibody Test: Better Than Laboratory Serological Testing?

Laine, Loren; Knigge, Kandice L.; Faigel, Douglas O.; Margaret, Nathan; Marquis, Scott; Vartan, Georgette; Fennerty, Brian

doi:10.1111/j.1572-0241.1999.01510.x

Cited by 14 publications

(3 citation statements)

References 11 publications

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“…All the evidence to date suggests that they have very much lower sensitivity and specificity compared with a laboratory‐based elisa test. Overall, greater accuracy can be obtained with laboratory‐based tests compared with near‐patient tests 68,69 which at present seem not accurate enough to be routinely recommended 64,68–81 ( Table 5,6).…”

Section: Non‐invasive Diagnosismentioning

confidence: 99%

Invasive and non‐invasive tests for Helicobacter pylori infection

Vaira

Holton

Menegatti

et al. 2000

Aliment Pharmacol Ther

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Summary There are two general ways in which a diagnosis of infection by Helicobacter pylori can be made: by using either an invasive or non‐invasive procedure. The invasive procedures involve an endoscopy and biopsy. A biopsy is essential because often the mucosa may appear macroscopically normal but nevertheless be inflamed. A biopsy is obtained by histological examination, culture, polymerase chain reaction or detection of the presence of urease activity in biopsy material. The non‐invasive tests that can be used to diagnose the infection are serology, detection of labelled metabolic products of urea hydrolysis in the breath (13CO2, 14CO2), the urine or the blood, and detection of H, pylori antigen in a stool specimen. At present no single test can be relied upon to detect definitely colonization by H. pylori, and a combination of two is recommended if this is feasible. The choice of the test to be used is not straightforward and may vary according to the clinical condition and local expertise.

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Section: Non‐invasive Diagnosismentioning

confidence: 99%

Invasive and non‐invasive tests for Helicobacter pylori infection

Vaira

Holton

Menegatti

et al. 2000

Aliment Pharmacol Ther

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“…The majority of human whole blood RNA stabilization/isolation kits require venous blood samples of a minimum of 0.5 ml, usually acquired by venipuncture samples. Medical applications using capillary blood include the monitoring of blood glucose to test bacterial infections, e.g., Helicobacter pylori (8,9) and to determine cholesterol (10,11) and other parameters. Capillary blood from fingertips was used for these tests.…”

Section: Introductionmentioning

confidence: 99%

Capillary earlobe blood may be used for RNA isolation, gene expression assays and microRNA quantification

Wehmeier

Hilberg

2013

Molecular Medicine Reports

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Abstract. An increasing number of studies examining gene expression associated with diseases in children is likely, in the near future, to provide simple and easy to use methods for the isolation of RNA for gene expression profiling. Prerequisites for such studies are likely to encompass the use of small amounts of blood, as well as less invasive blood collection methods. In the current study, RNA was isolated from 20 µl capillary blood samples from the earlobes of 10 adults for quantitative PCR experiments. The results were compared with RNA isolated from venipuncture samples of the 10 samples. The expression of 4 mRNAs and 1 microRNA (miRNA), miRNA-126, was measured. The quantitative PCR results obtained with the capillary blood probes were similar to results using venous blood samples. The few differences observed may result from a variation in the blood cell composition. The use of capillary blood samples from the earlobe for gene expression analysis is likely to allow this method to be used in newborns, babies and children. In addition, such a method, using microliters of blood samples, may also be useful for other medical studies e.g., in cases where repetitive blood sampling is necessary or in patients with bleeding disorders.

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“…A classic example of this methodology is blood glucose monitoring. Fingerstick blood has also been used to test for Helicobacter pylori infection [5,6], cholesterol [7,8], glycosylated hemoglobin (A1c) levels [9] and syphilis [9]. However, a review of the current literature reveals that there are no protocols available for extracting sufficient amounts of good quality total RNA from much smaller amounts of starting material for use in DNA microarray studies, specifically a droplet of blood from a finger stick (50-100 ul).…”

Section: Introductionmentioning

confidence: 99%

Whole genome transcript profiling from fingerstick blood samples: a comparison and feasibility study

et al. 2009

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BackgroundWhole genome gene expression profiling has revolutionized research in the past decade especially with the advent of microarrays. Recently, there have been significant improvements in whole blood RNA isolation techniques which, through stabilization of RNA at the time of sample collection, avoid bias and artifacts introduced during sample handling. Despite these improvements, current human whole blood RNA stabilization/isolation kits are limited by the requirement of a venous blood sample of at least 2.5 mL. While fingerstick blood collection has been used for many different assays, there has yet to be a kit developed to isolate high quality RNA for use in gene expression studies from such small human samples. The clinical and field testing advantages of obtaining reliable and reproducible gene expression data from a fingerstick are many; it is less invasive, time saving, more mobile, and eliminates the need of a trained phlebotomist. Furthermore, this method could also be employed in small animal studies, i.e. mice, where larger sample collections often require sacrificing the animal. In this study, we offer a rapid and simple method to extract sufficient amounts of high quality total RNA from approximately 70 μl of whole blood collected via a fingerstick using a modified protocol of the commercially available Qiagen PAXgene RNA Blood Kit.ResultsFrom two sets of fingerstick collections, about 70 uL whole blood collected via finger lancet and capillary tube, we recovered an average of 252.6 ng total RNA with an average RIN of 9.3. The post-amplification yields for 50 ng of total RNA averaged at 7.0 ug cDNA. The cDNA hybridized to Affymetrix HG-U133 Plus 2.0 GeneChips had an average % Present call of 52.5%. Both fingerstick collections were highly correlated with r2 values ranging from 0.94 to 0.97. Similarly both fingerstick collections were highly correlated to the venous collection with r2 values ranging from 0.88 to 0.96 for fingerstick collection 1 and 0.94 to 0.96 for fingerstick collection 2.ConclusionsOur comparisons of RNA quality and gene expression data of the fingerstick method with traditionally processed sample workflows demonstrate excellent RNA quality from the capillary collection as well as very high correlations of gene expression data.

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Fingerstick Helicobacter Pylori Antibody Test: Better Than Laboratory Serological Testing?

Abstract: New generation in-office, whole-blood antibody tests that can achieve a sensitivity and specificity similar to or better than those of widely used quantitative laboratory serological tests may be used as the initial screening tests of choice for H. pylori.

Cited by 14 publications

References 11 publications

Invasive and non‐invasive tests for Helicobacter pylori infection

Invasive and non‐invasive tests for Helicobacter pylori infection

Capillary earlobe blood may be used for RNA isolation, gene expression assays and microRNA quantification

Whole genome transcript profiling from fingerstick blood samples: a comparison and feasibility study

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