Withdrawal resistance of the mortise-and-tenon (M&T) joint is an important indicator to evaluate the strength of the M&T joint. Generally, the T-shaped specimen is usually used to measure withdrawal resistance using tensile methods, but there has not been testing standard until now. In this study, the methods of measuring withdrawal resistance of the M&T joint were investigated systematically. A new method used to determine the withdrawal resistance of M&T joint was proposed to be named as the compressive method. In addition, withdrawal resistances of M&T joint determined using tensile and compressive methods were compared based on experiments and the finite element method (FEM). The experimental results showed that the effects of the testing method and glue on withdrawal resistance of M&T joint were statistically significant, specifically, the withdrawal resistance measured using tensile method was higher than the one determined by the compressive method. Meanwhile, the results of FEM also confirmed the experimental results in a visible way. The results of FEM indicated that the withdrawal resistance of the M&T joint was influenced by tenon member length; specifically, the withdrawal resistance of the M&T determined by tensile method increased with the increasing of tenon member length. In summary, the results of the study make sense in the measurement of withdrawal resistance of the M&T joint in practice, and in f theory, the study will contribute to instructing the design of furniture structures.