Finite element analysis of the influence of cyclic strain on cells anchored to substrates with varying properties

Banerjee, Abhinaba; Khan, Mohammed Parvez; Barui, Ananya; Datta, Pallab; Chowdhury, Amit Roy; Bhowmik, Krishnendu

doi:10.1007/s11517-021-02453-4

Cited by 7 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typically, interactions between cells and deformable continuous substrates have been modelled by means of FE software (Dabagh et al 2017 ; Banerjee et al 2021 ). Solving large DNMs with implicit FE codes, however, can become cumbersome since the random connection between pin-jointed truss-like fibres can lead to sub-isostatic networks (Picu and Ganghoffer 2020 , Ch.…”

Section: Discussionmentioning

confidence: 99%

“…These models are usually three-dimensional and include representations of the nucleus, cytoplasm, membrane, nuclear membrane, and cytoskeletal components (e.g. Barreto et al 2013 ; Bansod et al 2018 ; Khunsaraki et al 2021 ; Banerjee et al 2021 ; Shen et al 2020 ; Jakka and Bursa 2021 ; Stracuzzi et al 2021 ). Typically, the cytoplasm and nucleus are represented as volume elements, whereas the membrane and nuclear membrane are represented by membrane or shell and the cytoskeleton by beam or truss elements.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Discrete network models of endothelial cells and their interactions with the substrate

Jakob,

Britt,

Giampietro

et al. 2024

Biomech Model Mechanobiol

View full text Add to dashboard Cite

Endothelial cell monolayers line the inner surfaces of blood and lymphatic vessels. They are continuously exposed to different mechanical loads, which may trigger mechanobiological signals and hence play a role in both physiological and pathological processes. Computer-based mechanical models of cells contribute to a better understanding of the relation between cell-scale loads and cues and the mechanical state of the hosting tissue. However, the confluency of the endothelial monolayer complicates these approaches since the intercellular cross-talk needs to be accounted for in addition to the cytoskeletal mechanics of the individual cells themselves. As a consequence, the computational approach must be able to efficiently model a large number of cells and their interaction. Here, we simulate cytoskeletal mechanics by means of molecular dynamics software, generally suitable to deal with large, locally interacting systems. Methods were developed to generate models of single cells and large monolayers with hundreds of cells. The single-cell model was considered for a comparison with experimental data. To this end, we simulated cell interactions with a continuous, deformable substrate, and computationally replicated multistep traction force microscopy experiments on endothelial cells. The results indicate that cell discrete network models are able to capture relevant features of the mechanical behaviour and are thus well-suited to investigate the mechanics of the large cytoskeletal network of individual cells and cell monolayers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discrete network models of endothelial cells and their interactions with the substrate

Jakob,

Britt,

Giampietro

et al. 2024

Biomech Model Mechanobiol

View full text Add to dashboard Cite

show abstract

Characterising the Mechanism of Cell Failure Due to Shear Using a Combined Numerical and Analytical Approach

Smart,

Hooker,

Bhattacharjee

et al. 2024

Mechanisms and Machine Science

View full text Add to dashboard Cite

Cell-Laden Alginate Hydrogel Modelling using Three-Dimensional (3D) Microscale Finite Element Technique

Banerjee

Chowdhury

Datta

et al. 2022

J. Inst. Eng. India Ser. C

Self Cite

View full text Add to dashboard Cite

A novel modelling technique using finite element analysis to mimic the mechanoresponse of cell-laden biomaterial is proposed for the use in bioinks and other tissue engineering applications. Here a hydrogel-based composite biomaterial specimen was used consisting of 5% (V/V) HeLa cells added to alginate solution (4% W/V) and another specimen with no living cell present in alginate solution(4% W/V). Tensile test experiments were performed on both the specimens with a load cell of 25 N. The specimens were bioprinted using an in-house developed three-dimensional (3D) bioprinter. To allow for the nonlinear hyperelastic behavior of the specimen, the specimens were loaded very slowly, at rates of 0.1 mm/min and 0.5 mm/min, during the tensile test. The microscale finite element models developed in Ansys were loaded with similar load rates and their responses were recorded. Both the model results were validated with the experiment results. A very good agreement between the finite element model and the tensile test experiment was observed under the same mechanical stimuli. Hence, the study reveals that bioprinted scaffold can be virtually modeled to obtain its mechanical characteristics beforehand.

show abstract

Finite element analysis of the influence of cyclic strain on cells anchored to substrates with varying properties

Cited by 7 publications

References 58 publications

Discrete network models of endothelial cells and their interactions with the substrate

Discrete network models of endothelial cells and their interactions with the substrate

Characterising the Mechanism of Cell Failure Due to Shear Using a Combined Numerical and Analytical Approach

Cell-Laden Alginate Hydrogel Modelling using Three-Dimensional (3D) Microscale Finite Element Technique

Contact Info

Product

Resources

About