FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis

Sun, Bin; Bhati, Kaushal Kumar; Song, Peizhe; Edwards, Ashleigh; Petri, Louise; Kruusvee, Valdeko; Blaakmeer, Anko; Dolde, Ulla; Rodrigues, Vandasue; Straub, Daniel; Yang, Junbo; Jia, Guifang; Wenkel, Stephan

doi:10.1371/journal.pgen.1010386

Cited by 35 publications

(25 citation statements)

References 35 publications

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“…The H 2 O 2 levels in the protoplasts transfected with GSTU17-RNAi, AOX1a-OX, or AKR4C9-OX vector were lower than that in the fio1 mutant (Figure 7c) well as in wild type (Supplementary under cold stress (Govindan et al, 2022;Wang et al, 2023); however, the crucial roles played by the FIO1-meditated m 6 A regulation of stress response are largely unknown. In this study, we show that FIO1, a recently identified mRNA m 6 A methyltransferase that regulates floral transition in Arabidopsis (Cai et al, 2023;Sun et al, 2022;Wang et al, 2022;Xu et al, 2022), is associated with salt tolerance by modulating m 6 A levels and the stability of salt stressresponsive genes. The salt-sensitive phenotypes of the fio1 mutant, as evidenced by significantly less fresh weight and lower survival rate compared to wild type upon salt stress treatment (Figures 1 and 7), together with the highly induced expression patterns of FIO1 in response to salt stress (Figure S1), indicate that FIO1-mediated m 6 A modification contributes to salt tolerance.…”

Section: Salt Sensitivity Of the Fio1 Mutant Is Associated With H 2 O...mentioning

confidence: 66%

“…FIO1 is a recently identified m 6 A writer responsible for m 6 A modification in a small groups of mRNA and regulates floral transition by affecting the stability and splicing of several flowering‐related genes in Arabidopsis (Cai et al, 2023; Sun et al, 2022; Wang et al, 2022; Xu et al, 2022). However, the role of FIO1‐mediated m 6 A methylation in stress response was unexplored.…”

Section: Introductionmentioning

confidence: 99%

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FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress

Cai,

Hu,

et al. 2024

Plant Cell & Environment

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N6‐methyladenosine (m6A) is an mRNA modification widely found in eukaryotes and plays a crucial role in plant development and stress responses. FIONA1 (FIO1) is a recently identified m6A methyltransferase that regulates Arabidopsis (Arabidopsis thaliana) floral transition; however, its role in stress response remains unknown. In this study, we demonstrate that FIO1‐mediated m6A methylation plays a vital role in salt stress response in Arabidopsis. The loss‐of‐function fio1 mutant was sensitive to salt stress. Importantly, the complementation lines expressing the wild‐type FIO1 exhibited the wild‐type phenotype, whereas the complementation lines expressing the mutant FIO1m, in which two critical amino acid residues essential for methyltransferase activity were mutated, did not recover the wild‐type phenotype under salt stress, indicating that the salt sensitivity is associated with FIO1 methyltransferase activity. Furthermore, FIO1‐mediated m6A methylation regulated ROS production and affected the transcript level of several salt stress‐responsive genes via modulating their mRNA stability in an m6A‐dependent manner in response to salt stress. Importantly, FIO1 is associated with salt stress response by specifically targeting and differentially modulating several salt stress‐responsive genes compared with other m6A writer. Collectively, our findings highlight the molecular mechanism of FIO1‐mediated m6A methylation in the salt stress adaptation.

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Section: Salt Sensitivity Of the Fio1 Mutant Is Associated With H 2 O...mentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress

Cai,

Hu,

et al. 2024

Plant Cell & Environment

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“…m 6 A is the most prominent and dynamic type of RNA methylation and determines the fate of CCG mRNA, including the mRNA stability, splice-site choice, and RNA 3′ end formation [ 13 , 120 ]. The importance of m 6 A in the regulation of the circadian clock in plants has been emphasized by several recent studies on the genome-wide analysis of the function of (i) CRY1/2-mediated MTA-MTB-FIP37 methylase complexes [ 112 , 120 ]; (ii) VIR, another conserved m 6 A writer complex component [ 13 ]; and (iii) FIO1, a homolog of the m 6 A writer METTL16, in the regulation of the circadian clock and related physiology ( Figure 2 ) [ 62 , 121 , 122 , 123 , 124 ].…”

Section: M 6 a In The Plant Circadian Rhythmmentioning

confidence: 99%

“…Recently, several reports have highlighted how FIO1 mediates m 6 A methylation by regulating various circadian clock-related developmental processes including photomorphogenesis and floral transition ( Figure 2 ) [ 62 , 121 , 122 , 123 , 124 ]. In fio1 mutants, about 10%–15% of m 6 A in the total mRNA and 10–60% of the U6 snRNAs were reduced in seedlings, rosette leaves, and floral buds, which were relatively minor effects compared to the 80%–90% m 6 A loss in fip37 and mta .…”

Section: M 6 a In The Plant Circadian Rhythmmentioning

confidence: 99%

“…Interestingly, there were no global pattern changes in the remaining m 6 A between the wild type and fio1 mutant, indicating that FIO1 has no preferential choice in m 6 A position and motif. FIO1 has a lesser effect on global m 6 A levels, but a higher m 6 A reduction in specific groups of mRNAs, indicating that FIO1 likely mediates certain biological and physiological processes [ 62 , 121 , 122 , 123 , 124 ]. Gene ontology analysis of the differentially expressed genes and genes with hypomethylated mRNA in fio1 mutant revealed characteristics of circadian rhythm and flower development [ 62 ].…”

Section: M 6 a In The Plant Circadian Rhythmmentioning

confidence: 99%

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Current Insights into m6A RNA Methylation and Its Emerging Role in Plant Circadian Clock

Chuong

Doan

Wang

et al. 2023

Plants

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N6-adenosine methylation (m6A) is a prevalent form of RNA modification found in the expressed transcripts of many eukaryotic organisms. Moreover, m6A methylation is a dynamic and reversible process that requires the functioning of various proteins and their complexes that are evolutionarily conserved between species and include methylases, demethylases, and m6A-binding proteins. Over the past decade, the m6A methylation process in plants has been extensively studied and the understanding thereof has drastically increased, although the regulatory function of some components relies on information derived from animal systems. Notably, m6A has been found to be involved in a variety of factors in RNA processing, such as RNA stability, alternative polyadenylation, and miRNA regulation. The circadian clock in plants is a molecular timekeeping system that regulates the daily and rhythmic activity of many cellular and physiological processes in response to environmental changes such as the day-night cycle. The circadian clock regulates the rhythmic expression of genes through post-transcriptional regulation of mRNA. Recently, m6A methylation has emerged as an additional layer of post-transcriptional regulation that is necessary for the proper functioning of the plant circadian clock. In this review, we have compiled and summarized recent insights into the molecular mechanisms behind m6A modification and its various roles in the regulation of RNA. We discuss the potential role of m6A modification in regulating the plant circadian clock and outline potential future directions for the study of mRNA methylation in plants. A deeper understanding of the mechanism of m6A RNA regulation and its role in plant circadian clocks will contribute to a greater understanding of the plant circadian clock.

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Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean

Zhang,

Liu,

Chen

et al. 2024

Advanced Science

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Under low blue light (LBL) conditions, soybean exhibits classic shade avoidance syndrome (SAS) with exaggerated stem elongation (ESE), leading to lodging and yield reduction in dense farming. Recently, mRNA modification by N6‐methyladenosine (m6A) has emerged as a crucial epigenetic mechanism regulating plant biological processes; however, its impact on shade avoidance remains unexplored. In this study, the double mutants, gmmtas, that are impaired in two m6A writer genes, GmMTAa and GmMTAb that encode m6A methyltransferases or m6A writers are generated. It is found that the gmmtas mutants showed a substantial reduction of m6A levels, a dwarf phenotype, and a diminished sensitivity to LBL. Further investigation of the gmmtas mutants demonstrates that GmMTA regulates shade avoidance response by altering the expression of GmCRY1s, GmSPAs, and GmCOP1s, resulting in increased accumulation of GmSTFs that are known to suppress the shad avoidance response in response to LBL in soybean. The findings reveal a novel molecular mechanism regulating shade resistance in soybean, providing insights into the epigenetic mechanisms of plant adaptation to changing light environments and paving the way for the development of shade‐tolerant soybean varieties.

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FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis

Cited by 35 publications

References 35 publications

FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress

FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress

Current Insights into m6A RNA Methylation and Its Emerging Role in Plant Circadian Clock

Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean

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FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis

Cited by 35 publications

References 35 publications

FIONA1‐mediated mRNA m6A methylation regulates the response of Arabidopsis to salt stress

FIONA1‐mediated mRNA m6A methylation regulates the response of Arabidopsis to salt stress

Current Insights into m6A RNA Methylation and Its Emerging Role in Plant Circadian Clock

Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean

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FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress

FIONA1‐mediated mRNA m⁶A methylation regulates the response of Arabidopsis to salt stress