Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells

Kwak, So Young; Choi, You-Youn; Na, H.G.; Bae, Chang Hoon; Song, S.-Y.; Kim, Y.-D.

doi:10.4193/rhin19.172

Cited by 4 publications

(3 citation statements)

References 25 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, FIP intoxication of Nile tilapia was associated with induction of cytokines expression that was decreased significantly in curcumin-supplemented fish. Similar mechanism of FIP toxicity was reported in human nasal epithelial cells, in which FIP was capable of increasing the expression of IL-1β, IL-8 and IL-6 through ERK1/2, p38 and NF-kB signalling pathways (Kwak et al, 2019). In Japanese quail, a significant overexpression in IL-6, TNF-α and NFk b in heart and lung tissues of birds after exposure to FIP toxicity which was reversed after recovery of exposure for 60 days (Khalil et al, 2019).…”

Section: Discussionsupporting

confidence: 74%

Immunological and antioxidant role of curcumin in ameliorating fipronil toxicity in Nile tilapia ( Oreochromis niloticus )

Abdelkhalek

El‐Adl

El‐Ashram

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

This study was carried out to investigate the ameliorating effect of dietary curcumin Curcuma longa (CUR) against the subacute toxicity of fipronil (FIP) on Oreochromis niloticus. One hundred and eighty fish were divided into six groups and kept for 30 days; the first group was kept as a control group fed on commercial diet, while the second and third groups were fed on commercial diets supplemented with 1% (CUR1) and 3% (CUR3) curcumin powder/kg diet respectively. The fourth (FIP), fifth (FIP1) and sixth (FIP3) experimental groups were intoxicated with FIP (1/10 96 h LC50), where (FIP1) and (FIP3) groups were fed on a commercial diet supplemented with 1% and 3% of curcumin powder respectively. Hepatorenal damage markers, immunological, tissue antioxidant parameters and anterior kidney expression of IL‐8, IL‐1β and TGF‐β1 genes were determined. Curcumin alleviated the deteriorative effects of FIP intoxication through decreasing hepatic and renal damage markers, improving serum respiratory burst and lysozyme activities. Curcumin provoked a marked decrease in hepatic and renal malondialdehyde and nitric oxide concentration with a significant improvement in tissues' antioxidant status in FIP‐intoxicated fish. Gene expression analysis revealed a significant down‐expression of pro‐inflammatory markers genes after supplementation of curcumin in FIP‐intoxicated fish. In conclusion, the use of 3% curcumin as a feed additive could be implemented to protect fish against the toxic effects of agrochemical wastes via restoring antioxidant and immunological parameters of intoxicated fish.

show abstract

Section: Discussionsupporting

confidence: 74%

Immunological and antioxidant role of curcumin in ameliorating fipronil toxicity in Nile tilapia ( Oreochromis niloticus )

Abdelkhalek

El‐Adl

El‐Ashram

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

show abstract

“…IF was employed to further verify the expression of MUC5AC in IL-13-stimulated JME/CF15 cells as previously described ( 26 ). Briefly, following fixation with 4% paraformaldehyde (Shanghai Aladdin Biochemical Technology Co., Ltd.) for 10 min at room temperature, the cells were incubated with 0.5% Triton X-100 (cat.…”

Section: Methodsmentioning

confidence: 99%

Formononetin ameliorates IL‑13‑induced inflammation and mucus formation in human nasal epithelial cells by activating the SIRT1/Nrf2 signaling pathway

2021

View full text Add to dashboard Cite

Formononetin has proven to be anti-inflammatory and able to alleviate symptoms of certain allergic diseases. The present study aimed to determine and elucidate the potential effects of formononetin in allergic rhinitis. JME/CF15 cells were pretreated with formononetin at different doses, followed by stimulation with IL-13. Cell Counting Kit-8 assay was performed to determine the cytotoxicity of formononetin. The expression levels of inflammation-related proteins, histamine, IgE, TNF-α, IL-1β, IL-6, granulocyte-macrophage colony-stimulating factor and eotaxin in IL-13-stimulated JME/CF15 cells were detected using ELISAs. The expression levels of phosphorylated-NF-κB p65, NF-κB p65 and cyclooxygenase-2 (Cox-2) were analyzed using western blotting. Reverse transcription-quantitative PCR, western blotting and immunofluorescence were performed to measure the levels of mucin 5AC oligomeric mucus/gel-forming. Expression levels of sirtuin 1 (SIRT1) and nuclear erythroid factor 2-related factor 2 (Nrf2) proteins were also measured using western blotting. The results of the present study revealed that formononetin exerted no cytotoxic effect on the viability of JME/CF15 cells. Following stimulation of JME/CF15 cells with IL-13, formononetin suppressed the upregulated expression levels of proinflammatory cytokines. IL-13-induced formation of mucus was also attenuated by formononetin treatment. Furthermore, it was found that the SIRT1/Nrf2 signaling pathway was activated in formononetin-treated JME/CF15 cells, whereas treatment with the SIRT1 inhibitor, EX527, reversed the effects of formononetin on IL-13-induced inflammation and mucus formation in JME/CF15 cells. In conclusion, the findings of the current study indicated that formononetin may activate the SIRT1/Nrf2 signaling pathway, thereby inhibiting IL-13-induced inflammation and mucus formation in JME/CF15 cells. These results suggested that formononetin may represent a promising agent for the treatment of allergic rhinitis.

show abstract

“…[2]. Mucins are the major components and contributors to the viscoelastic properties of mucus, and play an important role in maintaining the airway environment and function [3]. Prior studies have shown that regulation of mucin expression is an important strategy for the treatment of airway mucus hypersecretion in chronic inflammatory airway diseases [4].…”

Section: Introductionmentioning

confidence: 99%

Spectrum-effect relationship between HPLC fingerprints and inhibitory activity in MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine

Liu¹,

Pei²,

He³

et al. 2022

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To investigate the spectrum-effect relationship between HPLC fingerprints and the inhibitory effect on MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA).Methods: The fingerprints of 20 PRPCA batches were established using HPLC and their similarities or differences were analyzed using hierarchical cluster analysis (HCA) and principal component analysis (PCA). The inhibitory effects of MUC5AC mucin were evaluated in LPS-treated NCI-H292 cells. The spectrum-effect relationship between common chromatographic peaks and MUC5AC inhibition was established using a partial least squares-discriminant analysis (PLS-DA).Results: Fifteen common chromatographic peaks were identified by analyzing HPLC fingerprints, with uridine, tyrosine, uracil, and inosine found as possible markers to distinguish the PRPCA from different sources. Spectrum-effect relationship analysis showed that the chromatographic peaks 5, 6, 10 (vernine), 12 (5-hydroxymethylfurfural), 14 (tryptophan) and 15 (adenosine) were closely associated with the inhibitory effect on MUC5AC mucin.Conclusion: The spectrum-effect relationship between HPLC fingerprints and the inhibitory effect on MUC5AC mucin of PRPCA was successfully established in the present study. Our findings further reveal the material basis of PRPCA and provide an effective method for its quality control.

show abstract

Fipronil upregulates inflammatory cytokines and MUC5AC expression in human nasal epithelial cells

Cited by 4 publications

References 25 publications

Immunological and antioxidant role of curcumin in ameliorating fipronil toxicity in Nile tilapia ( Oreochromis niloticus )

Immunological and antioxidant role of curcumin in ameliorating fipronil toxicity in Nile tilapia ( Oreochromis niloticus )

Formononetin ameliorates IL‑13‑induced inflammation and mucus formation in human nasal epithelial cells by activating the SIRT1/Nrf2 signaling pathway

Spectrum-effect relationship between HPLC fingerprints and inhibitory activity in MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine

Contact Info

Product

Resources

About