The present study investigates thermophysiological comfort and heat stress management in hot working environments when using protective clothing of 1.2 clo. Ten cases are examined with five values of air temperature and two values of air relative humidity. Through an analysis of key parameters such as the Predicted Mean Vote (PMV) index, Predicted Percentage Dissatisfied (PPD) index, climatic condition categories, final sweat rates, and recommended water consumption guidelines, the study examines the interplay between environmental conditions and human physiology. Findings indicate that while protective clothing may offer comfort at lower temperatures, higher temperatures and humidity levels exacerbate discomfort and pose health risks. This research contributes to a better understanding of heat stress management and informs practical strategies for enhancing workplace safety in hot environments.