In view of the global sustainable development, it is imperative that supplementary cementing materials (SCM) be used for replacing cement in the concrete industry and several researchers have shown that mineral admixtures can enhance the workability of lightweight aggregate concrete (LWAC) mixture and its strength. In view of the beneficial effects of using SCM in LWAC, this article aims to verify the possible influence of the use of different types of SCM in the segregation phenomenon of LWAC. Three different SCM were studied: Silica Fume (SF), Fly Ash (FA) and Posidonia oceanica Ash (PA). For each SCM, three mixtures were prepared, considering three different percentage substitutions of cement. An image analysis technique was applied to estimate the segregation in each sample. The results show that a substitution of cement by other materials with different grain size, considering a constant water binder ratio, may also result in a variation of the consistency of concrete and the viscosity of the mortar matrix, which may contribute to increase or reduce segregation.