According to literature, practice and experience, the firing distance from shotguns (i.e., the distance between the victim and the muzzle of the gun) can be determined fairly accurately by studying and evaluating the pellet dispersion pattern after shooting a target surface, either on clothing or on the victim’s body. Many formulas have been published in order to determine the range at which a shotgun has been discharged, as well as the parameters that affect the calculation of the estimated firing distance. Τhe purpose of the current study was to provide answers to questions regarding firing distance ranges, by firing 12-gauge shotguns from 10, 18 and 25 meters, using different sizes of barrel choke and pellet sizes. Experimental shots were fired from two different guns, with different technical characteristics, from different distances and with different types of shotshell charges. The research revealed that 65% to 80% of the pellet pattern is distributed evenly in the center of the shot and this percentage remains the same at all three distances (10, 18 and 25 meters). Also, an important term in research is Shotgun Pellet Pattern (SPP), which is based on the number of pellets that are uniformly imprinted in the center of the shot and is an important factor in determining shot distance. The results of the shootings were recorded in tables and integrated into a computer program, giving useful information to the case examiner regarding the ability to determine the firing distance from a shotgun.