Firpronil induced spermotoxicity is associated with oxidative stress, DNA damage and apoptosis in male rats

Khan, Saleem A.; Jan, M.H.; Kumar, Dhirendra; Telang, A.G.

doi:10.1016/j.pestbp.2015.03.010

Cited by 55 publications

(23 citation statements)

References 44 publications

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“…Similar metabolic modulations have been observed in other cell types as well as mitochondria31474849; in addition, depending on the mode of exposure, these modulations can lead to cell death484950. We assume that these effects on the mortality and concentration of spermatozoa are due to physiological disruptions because an increase in energetic metabolism could lead to oxidative injuries by generating reactive oxygen species, as previously confirmed for Fipronil384950. Although spermatozoa exhibit antioxidant defenses51, an overload of these defenses can lead to cell death, explaining the high mortality rate of spermatozoa in drones exposed to Fipronil.…”

Section: Resultssupporting

confidence: 74%

“…The probability of having unfertilized eggs, from the exclusive release of dead spermatozoa, is especially important because the queen is economical in terms of sperm use64. If the queen releases only live spermatozoa, defects in brood production would potentially result from altered spermatozoa functional and structural integrity, particularly that of membranes or DNA, which have been shown to be affected by oxidative stress in rats after exposure to Fipronil38. DNA damage has also been observed in insect spermatozoa, especially in Drosophila, subsequent to a redox homeostasis impairment induced by an environmental stressor65.…”

Section: Resultsmentioning

confidence: 99%

“…This neurotoxic phenylpyrazole insecticide is known to induce lethal and sublethal effects at the cellular3132, behavioral33343536 and colonial level36 in honey bees as well as in non-target invertebrates37. Interestingly, Fipronil also alters the reproductive functions of some terrestrial and aquatic vertebrates and invertebrates3839404142. To study the impact on fertility, drones, which depend on workers for their food supply, were chronically exposed throughout their sexual maturation to a contaminated food source gathered by foragers.…”

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confidence: 99%

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Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential

Kairo¹,

Provost

Tchamitchian³

et al. 2016

Sci Rep

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A species that requires sexual reproduction but cannot reproduce is doomed to extinction. The important increasing loss of species emphasizes the ecological significance of elucidating the effects of environmental stressors, such as pesticides, on reproduction. Despite its special reproductive behavior, the honey bee was selected as a relevant and integrative environmental model because of its constant and diverse exposure to many stressors due to foraging activity. The widely used insecticide Fipronil, the use of which is controversial because of its adverse effects on honey bees, was chosen to expose captive drones in hives via syrup contaminated at 0.1 μg/L and gathered by foragers. Such environmental exposure led to decreased spermatozoa concentration and sperm viability coupled with an increased sperm metabolic rate, resulting in drone fertility impairment. Subsequently, unexposed queens inseminated with such sperm exhibited fewer spermatozoa with lower viability in their spermatheca, leaving no doubt about the detrimental consequences for the reproductive potential of queens, which are key for colony sustainability. These findings suggest that pesticides could contribute to declining honey bee populations through fertility impairment, as exemplified by Fipronil. More broadly, reproductive disorders should be taken into consideration when investigating the decline of other species.

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential

Kairo¹,

Provost

Tchamitchian³

et al. 2016

Sci Rep

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“…Sperm DNA integrity is a sign of the reproductive capability of the sperm [60]. Therefore, the DNA’s structural integrity needs to be investigated in order to evaluate sperm function and its structural changes [34].…”

Section: Discussionmentioning

confidence: 99%

Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress

et al. 2017

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Levetiracetam (LEV) is an antiepileptic drug commonly used in the treatment of epilepsy because of its excellent safety profile in all age groups. It is remarkable that there are no studies evaluating the toxic effects of this drug on the male reproductive system, as it is commonly used in male patients of reproductive age. From this point of view, our aim was to evaluate the possible toxic effects of LEV on the male reproductive system. Therefore, LEV was administered to male rats orally at 50, 150, and 300 mg/kg for 70 consecutive days. At the end of this period, alterations to body and organ weights were calculated, and sperm concentration, motility, and morphology were investigated by a computer-assisted sperm analysis system. Sperm DNA damage was determined by comet assay and histopathological examination of the testes was carried out. Serum testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels were measured by ELISAs to determine the effects of hormonal status, while glutathione, superoxide dismutase, catalase, and malondialdehyde levels in the testes were measured by colorimetric assay kits to determine the role of oxidative status in potential toxicity. According to the results, sperm quality was decreased by LEV treatment in a dose-dependent manner. LEV induced significant DNA damage in the 150 and 300 mg/kg LEV-administered groups. Histopathology of the testes showed that LEV resulted in testicular injury in the 300 mg/kg LEV-administered group. Serum testosterone, FSH, and LH levels were significantly decreased in the 300 mg/kg LEV-administered group. Glutathione, superoxide dismutase, and catalase levels were significantly decreased in all experimental groups while malondialdehyde levels were significantly increased in 150 and 300 mg/kg LEV-administered groups. According to these results, it was determined that LEV administration decreased sperm quality and it was alleged that hormonal alteration and oxidative stress are potential contributors to reproductive toxicity.

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“…Sperm DNA integrity is a sign of the reproductive capability of the sperm (Khan et al, ). Therefore, it needs to be determined to evaluate the function of sperm and its structural changes (Ong et al, ).…”

Section: Discussionmentioning

confidence: 99%

Citalopram Induces Reproductive Toxicity in Male Rats

Ilgın

Kılıç

Baysal

et al. 2017

Birth Defects Research

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Firpronil induced spermotoxicity is associated with oxidative stress, DNA damage and apoptosis in male rats

Cited by 55 publications

References 44 publications

Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential

Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential

Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress

Citalopram Induces Reproductive Toxicity in Male Rats

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