First and second derivative recording of thin-layer chromatograms

Traveset, J.; Such, V.; Gonzalo, R.; Gelpí, E.

doi:10.1016/s0021-9673(00)81638-6

Cited by 14 publications

(1 citation statement)

References 6 publications

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“…Even so, for special cases where the Chromatographie resolution cannot be readily improved, analytical techniques can be undertaken using a series of higher order derivative procedures. These can be applied especially in multiple peak recognition studies [63,[268][269][270][271] .…”

Section: Future Processing and Treatment Of High-resolution Gas Chrommentioning

confidence: 99%

Aspects of High-Resolution Gas Chromatography as Applied to the Analysis of Hydrocarbon Fuels and Other Complex Organic Mixtures. Volume 1

Rubey¹

1985

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Symmetric elution zone profile 3 Partially disengaged solute zones 4 Block diagram of basic systems approach 5 Three general forms of Chromatographie zone profiles Ig 6 Different combinations of solute zone profiles. 17 7 Rapidly generated chromatogram of a jet fuel. . 8 Chromatogram of a jet fuel obtained with a Pyrex glass open tubular column 9 Gaussian and exponentially modified Gaussian elution zone profiles 10 Series of normal paraffins eluted from a contaminated column 11 Elution behavior after removing contaminated section of column 12 Open tubular column gas chromatograms that exhibit unusual baseline behavior 13 Undesirable quantitative behavior 41 14 Relationship of three-component gas Chromatographie compromises 4 3 15 Relationship of admitted solute versus solute zone variance 44 16 Graphic procedures for measuring solute zone asymmetry 4 6

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Section: Future Processing and Treatment Of High-resolution Gas Chrommentioning

confidence: 99%

Aspects of High-Resolution Gas Chromatography as Applied to the Analysis of Hydrocarbon Fuels and Other Complex Organic Mixtures. Volume 1

Rubey¹

1985

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A novel approach to the qualitative and quantitative assay of HPTLC plates: Second and fourth derivative recording of spectrophotodensitograms

Traveset

Such

Gonzalo

et al. 1982

J. High Resol. Chromatogr.

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Key Words:Thin-layer chromatography, HPTLC Qualitative and quantitative analysis Derivative spectra of analytes SummaryOne of the limitations due to lackof resolution for a given pair of analytes in TLC or HPTLC is the need to optimize the system. In practice this requires time, rerunning of the sample in different developing solvents, and a great deal of expertise on the part of the analyst.In our experience, application of first and second derivative recording techniques toHPTLC facilitates and speeds the whole process, permitting qualitative and quantitative assay of most unresolved spots. Consequently, we have now extended our instrumental capabilities to fourth derivative measurements. For this purpose, we have added a homemade electronic unit in series with the one previously used for first and second order derivatives. Thus, we have been able to evaluate the potential advantages of higher order derivatives for HPTLC analysis of unresolved components in various pharmaceutical products. A comparison of second and fourth order derivative measurements of seriously overlapping HWLC components in a sample of preservatives used in the pharmaceutical industry suggests that the lower order derivatives might be a better choice in view of the higher accuracy and precision of the corresponding data. This is supported by the results of other applications, such as the assay of a commercial colorant, and a syrup formulation. The observed lack of precision of fourth order measurements stems from the fact that although the second and higher order derivatives produce narrower bandwidths, thus contributing to improved resolution, the signal to noise ratio decreases and satellite peak interactions increase, thus rendering correct discrimination of the fine structural detail of overlapping components more difficult.

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