First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free sperm vitrification protocol

Medrano, Llanos; Enciso, María; Gómez‐Torres, María José; Aizpurúa, Jon

doi:10.1016/j.cryobiol.2019.01.014

Cited by 7 publications

(10 citation statements)

References 10 publications

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“…This method can be applied in all techniques of in vitro fertilization in reproductive medicine programs including epididymal (MESA) and testicular (TESE) spermatozoa 18,46 …”

Section: Applications Of the Vitrification Methods In Human Medicinementioning

confidence: 99%

“…17,34,54 This method can be applied in all techniques of in vitro fertilization in reproductive medicine programs including epididymal (MESA) and testicular (TESE) spermatozoa. 18,46 Our group has shown that not only is motility properly preserved, as well as DNA integrity and membranes. 13,55 In addition,…”

Section: Applic Ations Of the Vitrific Ation Me Thod In H Uman Med mentioning

confidence: 99%

“…and testicular (TESE) spermatozoa. 18,46 Our group has shown that not only is motility properly preserved, as well as DNA integrity and membranes. 13,55 In addition, devitrified spermatozoa at room temperature better preserve viability, membrane mitochondrial potential and do not increase in phosphatidylserine externalization up to a period of 4 hours.…”

Section: Applic Ations Of the Vitrific Ation Me Thod In H Uman Med mentioning

confidence: 99%

“…With this method, percentages of motile spermatozoa have been obtained with low DNA fragmentation and preservation of plasma membrane integrity, 13 which makes it possible to use this technique in assisted reproduction procedures. 17,18 This review summarizes the research carried out on the technology of vitrification of human spermatozoa developed by our group, with the use of non-permeable cryoprotectants in microdrops and sealed straws, as well as the devitrification methodology, which allowed us to develop a patented methodology (US 9 005 886 B2, 2015), 19 which we have been perfecting to obtain better survival rates and sperm functionality post-vitrification.…”

Section: Introductionmentioning

confidence: 99%

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Human sperm vitrification: A scientific report

et al. 2020

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Background The sperm vitrification developed by this group is based on the ultrarapid freezing of a vitrification solution composed of a non‐permeable cryoprotectant (saccharides and protein), in which previously selected spermatozoa are resuspended, free of seminal plasma, and then plunged directly into liquid nitrogen. Compared to traditional sperm freezing, vitrification does not cause chemical or physical damage to the intracellular structures and reduces the damage to the plasma membrane because no ice crystals form, thus preserving motility and DNA integrity. Objectives This manuscript is a review of the vitrification methodology developed by the authors’ research group, including studies showing the application in human reproduction therapy. Materials and methods The authors perform a review of the work initiated more than a decade ago by this research group, on the implementation of sperm vitrification, a more effective technique for cryopreservation of human spermatozoa, discussing the results obtained by other authors and the projection of this technique. Results and discussion The vitrification technique has been developed in selected spermatozoa free of seminal plasma supplemented with saccharides such as sucrose, trehalose, and dextran, together with albumin, providing a high motility rate and protective structures of the cytoskeleton. In patients, it can be used to preserve their fertility for oncological reasons, genetics, inflammatory diseases, or reproductive medicine techniques. The possibility that vitrified spermatozoa can be preserved at temperatures of −80°C can simplify sample storage, optimizing the space and time as well as operator safety. Conclusion Vitrification techniques have demonstrated the preservation of selected spermatozoa without seminal plasma and with non‐permeable cryoprotectants and protein. Currently, it is one of the most effective ways to maintain sperm function and has been used in in vitro fertilization or intrauterine insemination in humans, achieving healthy live births.

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“…This method can be applied in all techniques of in vitro fertilization in reproductive medicine programs including epididymal (MESA) and testicular (TESE) spermatozoa 18,46 …”

Section: Applications Of the Vitrification Methods In Human Medicinementioning

confidence: 99%

Section: Applic Ations Of the Vitrific Ation Me Thod In H Uman Med mentioning

confidence: 99%

Section: Applic Ations Of the Vitrific Ation Me Thod In H Uman Med mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human sperm vitrification: A scientific report

et al. 2020

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“…Live births were achieved with vitrified spermatozoa by intracytoplasmic sperm injection (ICSI) [8] and by intrauterine insemination [9]. A healthy birth using a vitrified sperm sample was recently reported in Spain after a Day 5 blastocyst was monitored and transferred [10].…”

Section: Introductionmentioning

confidence: 99%

Human sperm vitrification: the state of the art

Tao

Sanger

Saewu

et al. 2020

Reprod Biol Endocrinol

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Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing.

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