First Case of Infection with Hantavirus Dobrava in Germany

Meisel, Helga; Lundkvist, Åke; Gantzer, K.; Bär, Werner; Sibold, Claus; Krüger, Detlev H.

doi:10.1007/s100960050214

Cited by 40 publications

(27 citation statements)

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“…We have recently iden- tified the first case of HFRS in Germany caused by DOB virus. 19 Our data show a geographically varied distribution of at least three hantaviruses in Slovakia. We have demonstrated the circulation of DOB virus in A. agrarius, as well as the occurrence of human DOB virus infections in this part of Europe.…”

Section: ͻ40mentioning

confidence: 58%

Short report: simultaneous occurrence of Dobrava, Puumala, and Tula Hantaviruses in Slovakia.

Sibold

Meisel²,

Lundkvist³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. The prevalence of antibody to hantaviruses in Slovakia (serum panel n ϭ 2,133) was lower in the western part (0.54%) and higher in the eastern part (1.91%) of the country and was found to be significantly enhanced in a group of forest workers from eastern Slovakia (5.88%). One-third of the IgM-negative convalescent phase sera from patients with hemorrhagic fever with renal syndrome exhibited antibodies reacting predominantly with Puumala virus antigen, while two-thirds had antibodies directed mainly against Hantaan virus antigen. Fine analysis of two Hantaan virus-reactive sera by a focus reduction neutralization test showed that Dobrava hantavirus was the source of these human infections. Initial results of rodent screening indicated the circulation of Dobrava virus in populations of striped field mice (Apodemus agrarius) in eastern Slovakia.

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Section: ͻ40mentioning

confidence: 58%

Short report: simultaneous occurrence of Dobrava, Puumala, and Tula Hantaviruses in Slovakia.

Sibold

Meisel²,

Lundkvist³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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“…Dozens of HFRS patients with DOBV infection have been diagnosed by typing their neutralizing antibodies, and the clinical parameters of these patients have been well characterized; in contrast to the situation in South-East Europe, HFRS cases caused by DOBV infection in Central Europe appear mostly mild or moderate, and no fatalities have been observed yet. On the other side, pulmonary involvement-sometimes even with life-threatening severity-was observed in a number of cases (26,27,48,49,51). Direct evidence for a causal role of DOBV-Aa in Central European HFRS cases was provided by amplification of a DOBV-Aa nucleotide sequence from an HFRS patient in Germany (16).…”

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confidence: 99%

Central European Dobrava Hantavirus Isolate from a Striped Field Mouse ( Apodemus agrarius )

et al. 2005

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Dobrava virus (DOBV) is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS) inEurope. It is hosted by at least two rodent species, Apodemus flavicollis and A. agrarius. According to their natural hosts they form the distinct genetic lineages DOBV-Af and DOBV-Aa, respectively. We have now established a DOBV isolate named Slovakia (SK/Aa) from an A. agrarius animal captured in Slovakia. The complete S and M and partial L segment nucleotide sequences of the new isolate were determined. Phylogenetic analyses showed that the SK/Aa isolate clustered together with the other DOBV-Aa sequences amplified from A. agrarius before and can be taken as the representative of this genetic lineage. SK/Aa, in comparison with a DOBV-Af isolate, was used for serotyping neutralizing antibodies of HFRS patients in Central Europe. Most patients' sera exhibited a higher endpoint titer when probed with our new isolate, suggesting that DOBV-Aa strains are responsible for most of the DOBV-caused HFRS cases in this region.Hantaviruses represent a unique genus Hantavirus within the Bunyaviridae family. They are "emerging viruses" that cause two human zoonoses: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome. The virus genome consists of three segments of negative-stranded RNA; the large (L) segment encodes the viral RNA-dependent RNA polymerase, the medium (M) segment the glycoprotein precursor (GPC), and the small (S) segment of the nucleocapsid (N) protein. In contrast to other genera of the Bunyaviridae family, hantaviruses are not transmitted by arthropods. Instead, they are spread by aerosolized rodent excreta and produce a chronic infection with no apparent harm in their natural hosts, rodents of the family Muridae (18,28,45,46). In Europe, at least two hantaviruses, Dobrava virus (DOBV) and Puumala virus (PUUV), are known to be pathogenic for humans (for reviews, see references 18 and 37). In addition, a single case of renal syndrome with pulmonary involvement has been very recently associated with infection by Tula virus (14). Currently, DOBV is intensively studied because of its unique properties; different virus lineages exist in different regions of Europe, and these lineages are harbored by different host reservoirs and probably display different levels of virulence in humans.A viable DOBV strain was isolated from lungs of a yellownecked mouse (Apodemus flavicollis) captured in a natural focus of HFRS in Dobrava village, Slovenia, South-East Europe (5). This virus isolate represents the DOBV prototype strain (named Slovenia or Slo/Af here) from A. flavicollis. Moreover, a second DOBV cell culture isolate (Ano-Poroia or AP/Af) has been established from an A. flavicollis mouse trapped in Greece (32, 34). Molecular phylogenetic analyses of nucleotide sequences from these cell culture-derived virus isolates, as well as from further South-East European A. flavicollis-associated strains, demonstrated their close mutual relationship (2, 4, 34, 35). These strains form a unique A. fla...

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“…In Germany two different serotypes of hantaviruses are known to be endemic. The predominant virus is PUU virus, mainly found in southwest Germany, which is carried by the bank vole (Clethrionomys glareolus) ( (12,16). In this part of Germany, DOB virus is found in Apodemus agrarius and seems to be less pathogenic to humans than Apodemus flavicollisderived DOB virus which is found in the Balkans (16).…”

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Hemorrhagic Fever with Renal Syndrome: Diagnostic Problems with a Known Disease

et al. 2001

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Hemorrhagic fever with renal syndrome (HFRS), caused by different hantaviruses, is a distinct clinical syndrome endemic in several parts of Asia and Europe. However, the clinical picture can sometimes be indistinguishable from that of other infectious or noninfectious diseases. In this report we describe a clinical case, which is a rare occurrence but is a prime example of the difficulties in the diagnosis of HFRS in areas with a low prevalence of the disease

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First Case of Infection with Hantavirus Dobrava in Germany

Cited by 40 publications

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Short report: simultaneous occurrence of Dobrava, Puumala, and Tula Hantaviruses in Slovakia.

Short report: simultaneous occurrence of Dobrava, Puumala, and Tula Hantaviruses in Slovakia.

Central European Dobrava Hantavirus Isolate from a Striped Field Mouse ( Apodemus agrarius )

Hemorrhagic Fever with Renal Syndrome: Diagnostic Problems with a Known Disease

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