First case of Trichinella nativa infection in wild boar in Central Europe—molecular characterization of the parasite

Bilska–Zając, Ewa; Różycki, Mirosław; Chmurzyńska, Ewa; Antolak, Ewelina; Próchniak, Marek; Grądziel-Krukowska, Katarzyna; Karamon, Jacek; Sroka, Jacek; Zdybel, Jolanta; Cencek, Tomasz

doi:10.1007/s00436-017-5446-6

Cited by 17 publications

(13 citation statements)

References 26 publications

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“…Homology search analyses of the selected markers revealed high level of sequence identity with the published sequences of T. spiralis and are in consent with previous studies (Wang et al, 2012;Bilska-Zając et al, 2017). T. spiralis has been recorded very frequently from the bordering country of India i.e.…”

Section: Resultssupporting

confidence: 88%

Molecular identification and characterization of Trichinella spiralis from a leopard in India

Nehra

Ram

Banerjee

et al. 2019

IJAR

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TPresent study describes species level identification of Trichinella spiralis of leopard origin from India using multiplex PCR and molecular characterization of the parasite based on sequencing of multiple genetic markers viz. 5S ribosomal DNA intergenic spacer region (5S ISR), partial mitochondrial large ribosomal subunit (Mt-lsr) and partial mitochondrial cytochrome c oxidase I (COI) genes. A single amplicons of 173 bp, indicative of T. spiralis was obtained in multiplex PCR. Further, specific PCR amplifications viz. 750 bp (5S ISR), 445 bp (Mt-lsr) and 850 bp (COI) were obtained for selected genetic markers. Homology search analysis of 5S-ISR, Mt-lsr gene and COI gene showed highest 99.6% identity with sequences originating from China (KT894074, T. spiralis), 98.6% similarity with T. spiralis China isolates (GU339127, GU339147) and 99.8% sequence homology with T. spiralis sequences originating from Belarus (MH119334), respectively. In the phylogenetic analysis, sequences of each selected genetic marker clustered together with published T. spiralis isolates only, which further confirmed species level identification of detected larvae as T. spiralis, although very few differences were noted with reference to relative positions. This is the first study from India, which provide molecular evidence on circulation of T. spiralis in wild animals.

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Section: Resultssupporting

confidence: 88%

Molecular identification and characterization of Trichinella spiralis from a leopard in India

Nehra

Ram

Banerjee

et al. 2019

IJAR

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“…The Western European country that had the largest number of literature available was Spain (13 articles), but few of these works were published recently (Díaz et al., 2021; Rodríguez‐Rodríguez et al., 2015). In central Europe, Italy had the most publications, with 10 articles (Badagliacca et al., 2016; Bandino et al., 2015; Marucci et al., 2021; Pozio et al., 2021; Ricchiuti et al., 2021), while in Eastern Europe, Poland had the largest number of studies, a number of which were published recently (Bień et al., 2016; Bilska‐Zając et al., 2016, 2017, 2020; Cybulska et al., 2016, 2019, 2020; Flis et al., 2017; Hurníková et al., 2016; Różycki et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

The genus Trichinella and its presence in wildlife worldwide: A review

Crisóstomo-Jorquera

Landaeta‐Aqueveque

2022

Transbounding Emerging Dis

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The genus Trichinella has a worldwide distribution, infecting people, domestic animals, and wildlife. It includes 13 genotypes, which are geographically delimited; Trichinella is transmitted to people through the ingestion of undercooked meat. Historically, it has been associated with pigs, but most Trichinella species affect wildlife, and cases of trichinellosis due to the consumption of game meat have been emerging. Therefore, it is important to monitor the sources of transmission to domestic animals and humans.The objective of this work was to analyse reports of Trichinella spp. in wild/feral animals around the world to identify the needs of future research in the epidemiology of the sylvatic cycle. A search of studies published until 2021 was conducted using Web of Science and SciELO. In the Palearctic, the most commonly studied hosts were wild boars and red foxes, and hosts with the highest prevalence rates were polar bears and martens. In the Nearctic, red foxes and black bears were the most frequently studied hosts, and the highest prevalence was found for wolverines and brown bears. In the Neotropics, positive reports were only identified in two countries, with wild boars being the most commonly studied species, and armadillos featuring the highest prevalence. In the Afrotropics, Trichinella limits its presence to Sub-Saharan Africa, where lions are the most studied hosts, and spotted hyenas have the highest prevalence. In the Indo-Malaya and Australasia ecozones, information on wildlife is scarce; the Norwegian rat is the most frequently studied host, and the Tasmanian devil has the highest prevalence of infection. In the last decade, research on world wildlife has increased which is associated with more frequent trichinellosis outbreaks caused by the consumption of wild meat. The results suggest the need to increase research in developing countries, particularly where more diverse sources of meat are available for human consumption.

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“…In Poland, wild boars are mainly infected with T. spiralis (78.31% of infections), followed by T. britovi (18.57%) and mixed infections T. spiralis/T. britovi-2.73% [37]. The other two species are rare: T. pseudospiralis (0.28%) and T. nativa (0.03%, only one case) were confirmed in wild boars [38].…”

Section: Discussionmentioning

confidence: 99%

Analysis of a Trichinellosis Outbreak in Poland after Consumption of Sausage Made of Wild Boar Meat

Różycki

Korpysa‐Dzirba

Bełcik

et al. 2022

JCM

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An outbreak of trichinellosis due to the consumption of sausage made from wild boar meat unexamined for the presence of Trichinella spp. was reported in Poland in December 2020. The outbreak affected eight people. Examination of the sausages made of wild boar meat collected during epidemiological investigation indicated a high level of Trichinella spp. Larvae per gram (>30 lpg) and therefore the threat of an infection in humans after consumption of such product was significant. Over the years, the main source of trichinellosis in Poland has been wild boar meat, and the majority of trichinellosis cases were related to the consumption of traditional raw meat products such as Polish sausage. Taking this into account, there is the need for better education of consumers in the Trichinella spp. endemic regions and among cultures consuming traditional raw meat products.

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First case of Trichinella nativa infection in wild boar in Central Europe—molecular characterization of the parasite

Cited by 17 publications

References 26 publications

Molecular identification and characterization of Trichinella spiralis from a leopard in India

Molecular identification and characterization of Trichinella spiralis from a leopard in India

The genus Trichinella and its presence in wildlife worldwide: A review

Analysis of a Trichinellosis Outbreak in Poland after Consumption of Sausage Made of Wild Boar Meat

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