First case report of infection due to Cupriavidus gilardii in a patient without immunodeficiency: a case report

Kobayashi, Takehito; Nakamura, Itaru; Fujita, Hiroaki; Tsukimori, Ayaka; Sato, Akihiro; Fukushima, Shinji; Ohkusu, Kiyofumi; Matsumoto, Tetsuya

doi:10.1186/s12879-016-1838-y

Cited by 23 publications

(21 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aeromonas , including A. veronii , are common water inhabitants and are often intrinsically resistant to carbapenems (Aubron et al., ; Lupo, Coyne, & Berendonk, ; Tacão et al., ). In contrast, this is the first time that carbapenem‐resistant C. gilardii isolates — which we identified in a location (W2) that uses recirculated potable water — have been reported outside of clinical settings (Karafin et al., ; Kobayashi et al., ).…”

Section: Discussionmentioning

confidence: 77%

Prevalence and characterization of carbapenem‐resistant bacteria in water bodies in the Los Angeles–Southern California area

et al. 2018

View full text Add to dashboard Cite

Carbapenems are β-lactam antibiotics used in healthcare settings as last resort drugs to treat infections caused by antibiotic-resistant bacteria. Carbapenem-resistant bacteria are increasingly being isolated from healthcare facilities; however, little is known about their distribution or prevalence in the environment, especially in the United States, where their distribution in water environments from the West Coast has not been studied before. The aim of this study was to determine the prevalence of carbapenem-resistant bacteria and carbapenemase genes in water bodies from the Los Angeles area (California, USA). All samples that were analyzed contained carbapenem-resistant bacteria with a frequency of between 0.1 and 324 carbapenem-resistant cfu per 100 mls of water. We identified 76 carbapenem-resistant or -intermediate isolates, most of which were also resistant to noncarbapenem antibiotics, as different strains of Enterobacter asburiae, Aeromonas veronii, Cupriavidus gilardii, Pseudomonas, and Stenotrophomonas species. Of them, 52 isolates were carbapenemase-producers. Furthermore, PCR and sequence analysis to identify the carbapenemase gene of these carbapenemase-producing isolates revealed that all Enterobacter asburiae isolates had a bla gene 100% identical to the reference sequence, and all Stenotrophomonas maltophlia isolates had a bla gene 83%-99% identical to the reference bla . Our findings indicate that water environments in Southern California are an important reservoir of bacteria-resistant to carbapenems and other antibiotics, including bacteria carrying intrinsic and acquired carbapenemase genes.

show abstract

Section: Discussionmentioning

confidence: 77%

Prevalence and characterization of carbapenem‐resistant bacteria in water bodies in the Los Angeles–Southern California area

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Patients infected with Cupriavidus species are usually immunocompromised [2,3,5,7]. Cases of infection with this genus have also been reported in elderly patients without obvious immunodeficiency [15,16]. The patient in the present study was immunocompetent, but had experienced frequent injuries to the lower limbs.…”

Section: Discussionmentioning

confidence: 51%

Cellulitis Caused by a Novel Cupriavidus Species Strain J1218 Identified by Whole Genome Sequencing

Yoon

Lim

et al. 2019

Ann Clin Microbiol

View full text Add to dashboard Cite

We report a case of cellulitis caused by a novel Cupriavidus species identified using whole-genome sequence analysis. Subcutaneous tissue biopsies from the left lower leg of a 67-year-old man who suffered from cellulitis were cultured. Round, convex, gray and non-hemolytic colonies were recovered after 72-h incubation. 16S rRNA sequence analysis showed 98.6% similarity with Cupriavidus basilensis DSM 11853(T) in the NCBI database and 99.9% similarity with C. basilensis KF708 in the EzBioCloud database. Genomic analysis using the MiSeq platform (Illumina, USA) and the TrueBac ID database (ChunLab, Korea) revealed that the average nucleotide identity (ANI) of this strain with C. basilensis DSM 11853(T) was 87.6%. The patient was treated with oral cefditoren pivoxil for 9 weeks. This study is the first to report cellulitis caused by Cupriavidus species strain J1218. (Ann Clin Microbiol 2019;22:105 -109)

show abstract

“…The third most abundant CR soil isolates obtained belonged to the genus Cupriavidus , which we identified in four different samples. Members of this genus are usually found in soil or water environments and occasionally as opportunistic pathogens (Coenye et al, 1999; Coenye, Goris, Spilker, Vandamme, & LiPuma, 2002; Harmon et al, 2019; Henriques et al, 2012; Hrenovic et al, 2019; Karafin et al, 2010; Kobayashi et al, 2016; Wang et al, 2015). However, carbapenem‐resistant C. alkaliphilus isolates have not been reported before in either clinical or environmental samples.…”

Section: Discussionmentioning

confidence: 99%

Urban and agricultural soils in Southern California are a reservoir of carbapenem‐resistant bacteria

et al. 2020

View full text Add to dashboard Cite

Carbapenems are last‐resort β‐lactam antibiotics used in healthcare facilities to treat multidrug‐resistant infections. Thus, most studies on identifying and characterizing carbapenem‐resistant bacteria (CRB) have focused on clinical settings. Relatively, little is still known about the distribution and characteristics of CRBs in the environment, and the role of soil as a potential reservoir of CRB in the United States remains unknown. Here, we have surveyed 11 soil samples from 9 different urban or agricultural locations in the Los Angeles–Southern California area to determine the prevalence and characteristics of CRB in these soils. All samples tested contained CRB with a frequency of <10 to 1.3 × 104 cfu per gram of soil, with most agricultural soil samples having a much higher relative frequency of CRB than urban soil samples. Identification and characterization of 40 CRB from these soil samples revealed that most of them were members of the genera Cupriavidus, Pseudomonas, and Stenotrophomonas. Other less prevalent genera identified among our isolated CRB, especially from agricultural soils, included the genera Enterococcus, Bradyrhizobium, Achromobacter, and Planomicrobium. Interestingly, all of these carbapenem‐resistant isolates were also intermediate or resistant to at least 1 noncarbapenem antibiotic. Further characterization of our isolated CRB revealed that 11 Stenotrophomonas, 3 Pseudomonas, 1 Enterococcus, and 1 Bradyrhizobium isolates were carbapenemase producers. Our findings show for the first time that both urban and agricultural soils in Southern California are an underappreciated reservoir of bacteria resistant to carbapenems and other antibiotics, including carbapenemase‐producing CRB.

show abstract

First case report of infection due to Cupriavidus gilardii in a patient without immunodeficiency: a case report

Cited by 23 publications

References 8 publications

Prevalence and characterization of carbapenem‐resistant bacteria in water bodies in the Los Angeles–Southern California area

Prevalence and characterization of carbapenem‐resistant bacteria in water bodies in the Los Angeles–Southern California area

Cellulitis Caused by a Novel Cupriavidus Species Strain J1218 Identified by Whole Genome Sequencing

Urban and agricultural soils in Southern California are a reservoir of carbapenem‐resistant bacteria

Contact Info

Product

Resources

About