First Chromatographic Isolation of an Antifungal Thaumatin-like Protein from French Bean Legumes and Demonstration of Its Antifungal Activity

Ye, X.Y.; Wang, H.X.; Ng, Tzi Bun

doi:10.1006/bbrc.1999.1166

Cited by 144 publications

(96 citation statements)

References 23 publications

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“…After the mycelial colony developed, discs impregnated with 2 mg of hexane extract were placed at a distance of 10 mm away from the Petri dishes inside limit. Then, they were incubated at 23°C for 72 h until mycelial growth had enveloped discs containing the control and had formed crescents of inhibition around discs containing samples with antifungal activity (Ye et al, 1999). However, Ketoconazol (7 µg/disk) was used as a positive control.…”

Section: Antifungal Activitymentioning

confidence: 99%

Evaluation of the antimicrobial activity of Acalypha monostachya Cav. (Euphorbiales: Euphorbiaceae)

Canales¹

2011

Afr. J. Pharm. Pharmacol.

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Acalypha monostachya (Euphorbiaceae) is used by the inhabitants of San Rafael and ZapotitlanSalinas, Puebla, Mexico, to alleviate illnesses of bacterial and fungal origin. We analyzed the antimicrobial activity of A. monostachya and some of its chemical features. The extracts were obtained by percolation. The antibacterial and antifungal activities were examined through the disc-diffusion method. The general toxicity was carried out by using Artemia salina. The quenching of free radicals by extracts was evaluated by the decolouration of a methanol solution of DPPH·. Total phenols were determined by Folin Ciocalteu reagent. Gram-positive and negative bacteria were sensitive to methanol and hexane extracts. The methanol extract had a bactericidal effect on Vibrio cholera Tor (MIC = 1000 µg/mL). The fungi strain most sensitive to this extract was T. mentagrophytes (LC 50 = 430 µg/mL). The methanol extract showed inhibition of DPPH· radical (SC 50 = 3.45 µg/ml), 43.4% of total phenolics and was toxic against A. salina (LC 50 = 4.5 µg/mL). The methanol extract showed a variety of phenolic compounds, while hexane extract showed different fatty acids methyl ester. The bacterial, antifungal, antioxidant activities and a qualitative chemical characterization of A. monostachya's extracts are reported here for the first time.

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Section: Antifungal Activitymentioning

confidence: 99%

Evaluation of the antimicrobial activity of Acalypha monostachya Cav. (Euphorbiales: Euphorbiaceae)

Canales¹

2011

Afr. J. Pharm. Pharmacol.

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“…PR-5 proteins share significant amino acid homology to thaumatin (a sweet-tasting [to humans] protein from the South African ketemfe berry bush) and are known as TL proteins. TL proteins have been isolated from A. thaliana (59,60), corn (62,148), soybeans, rice, wheat, tobacco (81), tomato (161), pumpkin (21), beans (196), barley (55), flax (12), and many other plants (122,182,184). The majority of PR-5 proteins have molecular masses of ϳ22 kDa and are stabilized by eight disulfide bonds.…”

Section: Fungal Cell Wall Structurementioning

confidence: 99%

Antifungal Proteins

Selitrennikoff

2001

Appl Environ Microbiol

529

362

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“…The mechanism of action of PR protein classes 1 and 4 remains unknown. Class 5 PR proteins include a number of proteins called thaumatin-like proteins [8][9][10]. Several constitutively expressed antifungal proteins include cysteine-rich small proteins [11,12], thionins [13], ribosome inactivating proteins [14], lipid-transfer proteins [15,16], chitin binding proteins [17,18], and proteinase inhibitors.…”

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confidence: 99%

Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica

Thippeswamy

Shivakameshwari

et al. 2003

Biochemical and Biophysical Research Communications

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In this study an antifungal protein from Urginea indica bulbs was purified to homogeneity by acid precipitation, Diol 300 Gelfiltration, and C 18 reverse phase HPLC. Its molecular mass was estimated to be 29 kDa and periodic acid-Schiff (PAS) staining showed that identified antifungal molecule is a glycoprotein. The neutralization of antifungal activity after periodate oxidation of 29 kDa glycoprotein suggests that the glycan part of the molecule appears to be involved in antifungal activity. N-terminal amino acid sequence of the purified protein was determined as SQLKAXIXDF. This sequence had no sequence similarity with any antifungal proteins. A polyclonal antiserum was raised against purified protein and used in immunolocalization analysis. Results suggest that it is localized to the cell wall of the bulb. Antifungal tests have demonstrated that U. indica protein exerts a fungistatic effect. It completely inhibits the germination of spores and hyphal growth of Fusarium oxysporum.

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First Chromatographic Isolation of an Antifungal Thaumatin-like Protein from French Bean Legumes and Demonstration of Its Antifungal Activity

Cited by 144 publications

References 23 publications

Evaluation of the antimicrobial activity of Acalypha monostachya Cav. (Euphorbiales: Euphorbiaceae)

Evaluation of the antimicrobial activity of Acalypha monostachya Cav. (Euphorbiales: Euphorbiaceae)

Antifungal Proteins

Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica

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