First comparative evaluation of a new leukapheresis technology in non‐cytokine‐stimulated donors

Steininger, Philipp; Strasser, Erwin; Weiss, Dominik R.; Achenbach, Susanne; Zimmermann, Robert; Eckstein, Reinhold

doi:10.1111/vox.12102

Cited by 30 publications

(40 citation statements)

References 26 publications

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“…Some authors could demonstrate that the CBC values performed just before starting leukocytapheresis of all cell populations correlated significantly with their corresponding cell yield in the product . However, other authors found strong, medium, and no correlation between the cell counts obtained with CBC before performing leukocytapheresis and the number of cells collected. Taken together all previous data, there seem to be further variables, which influence the correlation between pre‐apheresis values and the corresponding cell yield.…”

Section: Discussionmentioning

confidence: 99%

Leukocytapheresis in nonmobilized donors for cellular therapy protocols: Evaluation of factors affecting collection efficiency of cells

Cid

Carbassé

Alba

et al. 2019

J of Clinical Apheresis

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Introduction Collection efficiency (CE1) of cells refers to the number of cells that are collected from the total number of cells processed by the apheresis device. Limited data are available about the CE1 of cells when performing leukocytapheresis in nonmobilized donors for cellular therapy purposes. The aim of our study was to evaluate donor‐ and procedure‐related characteristics that might influence the CE1 of cells. Material and Methods Variables that predicted the CE1 of cells were analyzed by longitudinal linear regression in a series of 1071 leukocytapheresis procedures on 249 nonmobilized donors. Donor‐related characteristics considered were gender, age, total blood volume, and complete blood count (CBC) data. Procedure‐related characteristics considered were vascular access and device used. Results Older donor age was associated with a decrease in the CE1 of leukocytes, lymphocytes, monocytes, and mononuclear cells (MNCs; sum of leukocytes and monocytes) and with an increase in the CE1 of platelets (P < .05). Preprocedure CBC data (leukocytes, lymphocytes, monocytes, and platelets) were associated with either a statistically significant increase or decrease in the CE1 of cells. Central line used as vascular access was associated with a statistically significant decrease in the CE1 of MNCs (P = .02). Conclusion Donor's age, preprocedure CBC data as well as central line used as vascular access were factors associated with CE1 of cells. Knowing these characteristics is helpful in the apheresis unit when designing cellular therapy protocols in order to maximize the CE1 of the desired cell and to personalize collection variables for each donor.

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Section: Discussionmentioning

confidence: 99%

Leukocytapheresis in nonmobilized donors for cellular therapy protocols: Evaluation of factors affecting collection efficiency of cells

Cid

Carbassé

Alba

et al. 2019

J of Clinical Apheresis

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“…Both are based on Ficoll density centrifugation, and significant evidence of their suitability has been provided. The Spectra Optia BMP is a new application for the relatively recent Spectra Optia apheresis device that was previously introduced . The package consists of the standard Spectra Optia MNC filler, the standard Spectra Optia IDL tubing set, and a large BM bag for recirculating of the (increasingly MNC‐deplete) BM suspension, as well as a software that provides fully automatic, photo detector–guided apheresis of the BM, physically highly analogous to MNC apheresis in stem cell donors.…”

mentioning

confidence: 99%

Red blood cell depletion from bone marrow and peripheral blood buffy coat: a comparison of two new and three established technologies

et al. 2015

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“…In contrast, Punzel et al [10] detected only a weak correlation between the CD3+ pre-apheresis values and the CD3+ cell yield in the product. Steininger et al [11] could show a significant correlation between the pre-apheresis cell count and the correspondent product cell yield for CD14+ but not for CD3+ cells. Taken together, there seem to be further variables, which influence the correlation between pre-apheresis values and the corresponding cell yield.…”

Section: Discussionmentioning

confidence: 99%

“…Notwithstanding the above, the CMNC program remains easier to monitor and can be controlled more precisely than the MNC program. In addition, previous studies focused mainly on the differences in leukapheresis procedures and their performance [11,12]. Only a few publications included the short-term impact of the procedure on the donor [5,13].…”

Section: Introductionmentioning

confidence: 99%

Low-Volume Leukapheresis in Non-Cytokine-Stimulated Donors for the Collection of Mononuclear Cells

Anyanwu

Sitzmann

Hetjens

et al. 2018

Transfus Med Hemother

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Background: There is an increasing demand for products containing mononuclear cells (MNCs) for cellular immune therapy. Hence, leukapheresis is increasingly performed in healthy volunteer donors. Methods: We evaluated 147 low-volume leukapheresis procedures from 77 healthy non-cytokine-stimulated donors. Complete blood counts (CBCs) of the donors were measured before and directly after the procedures as well as from the MNC products. Follow-up CBCs were collected from donors within 21 days. Results: The product hematocrit within a range from 1.2 to 6.0% did not correlate with the collection efficiency of any cell population or the granulocyte and platelet yield. There was a strong correlation between the CBC values before leukapheresis and the cell yield of lymphocytes and monocytes as well as a perfect negative correlation between cell recruitment and cell loss in all cell populations. Furthermore, we observed a significant decrease in the CBC values in all cell populations directly after leukapheresis, which recovered within a mean of 16.1 days (SD ± 2.1 days) and even showed a significant increase in granulocytes and platelets. Conclusion: Low-volume leukapheresis is feasible for the collection of MNCs in which the product hematocrit is negligible for the collection efficiency, cell yield, or contamination of residual cells under operational settings recommended by the manufacturer. Our data suggests that cell recruitment is regulated by the number of cells removed, which may also be the stimulus to induce granulo- and thrombopoiesis within the first days after leukapheresis.

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First comparative evaluation of a new leukapheresis technology in non‐cytokine‐stimulated donors

Cited by 30 publications

References 26 publications

Leukocytapheresis in nonmobilized donors for cellular therapy protocols: Evaluation of factors affecting collection efficiency of cells

Leukocytapheresis in nonmobilized donors for cellular therapy protocols: Evaluation of factors affecting collection efficiency of cells

Red blood cell depletion from bone marrow and peripheral blood buffy coat: a comparison of two new and three established technologies

Low-Volume Leukapheresis in Non-Cytokine-Stimulated Donors for the Collection of Mononuclear Cells

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