First Complete Genome Sequence of Human Coronavirus HKU1 from a Nonill Bat Guano Miner in Thailand

Joyjinda, Yutthana; Rodpan, Apaporn; Chartpituck, Pongtorn; Suthum, Krairerk; Yaemsakul, Suphaluk; Cheun-Arom, Thaniwan; Bunprakob, Saowalak; Olival, Kevin J.; Stokes, Martha M.; Hemachudha, Thiravat; Wacharapluesadee, Supaporn

doi:10.1128/mra.01457-18

Cited by 10 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, most sequences in this study were obtained by consensus PCR using primers targeting highly conserved regions. Even if this broadly reactive PCR assay designed to detect widely variant CoVs has proven its ability to detect a large diversity of CoVs in a wide diversity of bats and mammals 30 , 74 – 77 , we may not rule out that some bat-CoV variants remained undetected. Using deep sequencing techniques would allow to detect this unknown and highly divergent diversity.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Origin and cross-species transmission of bat coronaviruses in China

et al. 2020

Self Cite

View full text Add to dashboard Cite

Bats are presumed reservoirs of diverse coronaviruses (CoVs) including progenitors of Severe Acute Respiratory Syndrome (SARS)-CoV and SARS-CoV-2, the causative agent of COVID-19. However, the evolution and diversification of these coronaviruses remains poorly understood. Here we use a Bayesian statistical framework and a large sequence data set from bat-CoVs (including 630 novel CoV sequences) in China to study their macroevolution, cross-species transmission and dispersal. We find that host-switching occurs more frequently and across more distantly related host taxa in alpha-than beta-CoVs, and is more highly constrained by phylogenetic distance for beta-CoVs. We show that inter-family and-genus switching is most common in Rhinolophidae and the genus Rhinolophus. Our analyses identify the host taxa and geographic regions that define hotspots of CoV evolutionary diversity in China that could help target bat-CoV discovery for proactive zoonotic disease surveillance. Finally, we present a phylogenetic analysis suggesting a likely origin for SARS-CoV-2 in Rhinolophus spp. bats.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Origin and cross-species transmission of bat coronaviruses in China

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…An additional study, that directly supported the transmission of the coronaviruses to humans without the intervention of intermediate hosts, was performed by Wacharapluesadee et al 34 . They identified HKU1 betacoronavirus in guano of bats in Thailand, and detected its presence in a non-hill individual with high level of occupational exposure to guano.…”

Section: The Puzzle Of the Direct Spillovermentioning

confidence: 99%

“…They identified HKU1 betacoronavirus in guano of bats in Thailand, and detected its presence in a non-hill individual with high level of occupational exposure to guano. The authors of the study reported that the contamination of the individual with the HKU1 virus was likely to have occurred through a human-to-human transmission rather than through exposure to bats, underlining the high risk of zoonotic spillover to people collecting guano 34 .…”

Section: The Puzzle Of the Direct Spillovermentioning

confidence: 99%

COVID19: an announced pandemic

2020

View full text Add to dashboard Cite

A severe upper respiratory tract syndrome caused by the new coronavirus has now spread to the entire world as a highly contagious pandemic. The large scale explosion of the disease is conventionally traced back to January of this year in the Chinese province of Hubei, the wet markets of the principal city of Wuhan being assumed to have been the specific causative locus of the sudden explosion of the infection. A number of findings that are now coming to light show that this interpretation of the origin and history of the pandemic is overly simplified. A number of variants of the coronavirus would in principle have had the ability to initiate the pandemic well before January of this year. However, even if the COVID-19 had become, so to say, ready, conditions in the local environment would have had to prevail to induce the loss of the biodiversity’s “dilution effect” that kept the virus under control, favoring its spillover from its bat reservoir to the human target. In the absence of these appropriate conditions only abortive attempts to initiate the pandemic could possibly occur: a number of them did indeed occur in China, and probably elsewhere as well. These conditions were unfortunately present at the wet marked in Wuhan at the end of last year.

show abstract

“…HCoVs in Thai children were also found in a large multi-center, multi-country study of respiratory viruses and influenza-like illness, and while all four endemic viruses were detected across the study, there was no country specific breakdown of coronavirus species detected ( Taylor et al, 2017 ). Most recently, a HCoV-HKU1 virus was detected in a non-ill bat guano miner ( Joyjinda et al, 2019 ). All of these studies support the ongoing circulation of these HCoVs, and their association with respiratory illness.…”

Section: Endemic Human Coronaviruses In Thailandmentioning

confidence: 99%

Review a brief history of coronaviruses in Thailand

Smith

2021

Journal of Virological Methods

View full text Add to dashboard Cite

First Complete Genome Sequence of Human Coronavirus HKU1 from a Nonill Bat Guano Miner in Thailand

Abstract: Human coronavirus HKU1 (HCoV-HKU1) was first detected in a patient with viral pneumonia from Hong Kong in 2004. Here, we report the first complete genome sequence of HCoV-HKU1 from Thailand, obtained from a nonill person who worked in a bat cave.

Cited by 10 publications

References 8 publications

RETRACTED ARTICLE: Origin and cross-species transmission of bat coronaviruses in China

RETRACTED ARTICLE: Origin and cross-species transmission of bat coronaviruses in China

COVID19: an announced pandemic

Review a brief history of coronaviruses in Thailand

Contact Info

Product

Resources

About