First Comprehensive Low-Density Horse Linkage Map Based on Two 3-Generation, Full-Sibling, Cross-Bred Horse Reference Families

Swinburne, June; Gerstenberg, Cornelia; Breen, Matthew; Aldridge, V.; Lockhart, Lesley; Marti, Eliane; Antczak, Douglas F.; Eggleston-Stott, M L; Bailey, E.; Mickelson, James R.; Røed, Knut; Lindgren, Gunnar; Haeringen, Wim von; Guérin, Gérard; Bjarnason, Jon; Allen, Twink; Binns, M. M.

doi:10.1006/geno.2000.6207

Cited by 84 publications

(73 citation statements)

References 27 publications

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“…Significant strides have since been made in expanding the gene map of the horse (Equus caballus; ECA, reviewed in Chowdhary and Raudsepp 2000). Currently ∼1200 markers have been mapped/assigned to various equine chromosomes using approaches such as synteny analysis (Caetano et al 1999a,b;Shiue et al 1999), genetic linkage mapping (Lindgren et al 1998;Guérin et al 1999Guérin et al , 2003Swinburne et al 2000a), and fluorescent in situ hybridization (FISH; e.g., Raudsepp et al 1999;Godard et al 2000;Lear et al 2001;Mariat et al 2001;Milenkovic et al 2002). Among the recent prominent developments are the generation of preliminary radiation hybrid (RH) maps for chromosomes ECA1 and ECA10 (Kiguwa et al 2000) and RH and comparative maps for some of the other equine chromosomes Raudsepp et al 2002).…”

Section: Tammen]mentioning

confidence: 99%

The First-Generation Whole-Genome Radiation Hybrid Map in the Horse Identifies Conserved Segments in Human and Mouse Genomes

Chowdhary¹,

Raudsepp²,

Kata³

et al. 2003

Genome Res.

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137

130

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A first-generation radiation hybrid (RH) map of the equine (Equus caballus) genome was assembled using 92 horse × hamster hybrid cell lines and 730 equine markers. The map is the first comprehensive framework map of the horse that (1) incorporates type I as well as type II markers, (2) integrates synteny, cytogenetic, and meiotic maps into a consensus map, and (3) provides the most detailed genome-wide information to date on the organization and comparative status of the equine genome. The 730 loci (258 type I and 472 type II) included in the final map are clustered in 101 RH groups distributed over all equine autosomes and the X chromosome. The overall marker retention frequency in the panel is ∼21%, and the possibility of adding any new marker to the map is ∼90%. On average, the mapped markers are distributed every 19 cR (4 Mb) of the equine genome-a significant improvement in resolution over previous maps. With 69 new FISH assignments, a total of 253 cytogenetically mapped loci physically anchor the RH map to various chromosomal segments. Synteny assignments of 39 gene loci complemented the RH mapping of 27 genes. The results added 12 new loci to the horse gene map. Lastly, comparison of the assembly of 447 equine genes (256 linearly ordered RH-mapped and additional 191 FISH-mapped) with the location of draft sequences of their human and mouse orthologs provides the most extensive horse-human and horse-mouse comparative map to date. We expect that the foundation established through this map will significantly facilitate rapid targeted expansion of the horse gene map and consequently, mapping and positional cloning of genes governing traits significant to the equine industry.

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Section: Tammen]mentioning

confidence: 99%

The First-Generation Whole-Genome Radiation Hybrid Map in the Horse Identifies Conserved Segments in Human and Mouse Genomes

Chowdhary¹,

Raudsepp²,

Kata³

et al. 2003

Genome Res.

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137

130

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“…The first equine linkage map included 140 markers (LINDGREN et al, 1998) and a more extensive linkage map consisted of 161 loci in 29 linkage groups for 26 autosomes . The first map covering all autosomes and the sexchromosomes using 353 microsatellite-markers was generated by SWINBURNE et al (2000). The microsatellites were mapped to 42 linkage groups and covered 1780 cM of the horse genome.…”

Section: Linkage Mapmentioning

confidence: 99%

Mapping the horse genome and its impact on equine genomics for identification of genes for monogenic and complex traits – a review

Stübs

Distl

2007

Arch. Anim. Breed.

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Since the beginning of investigation in the horse genome in the early nineties, there has been a great progress, especially during the last five years. At the beginning the exploration of monogenic hereditary diseases was one of the main aims, and the causal mutations of several diseases in the horse have been unravelled. The inheritance of coat colours has been explored very detailed, and there exist gene tests for different coat colours. Information about coat colours and inherited diseases is very important for the breeders and helps avoiding the appearance of lethal genetic factors or undesirable diseases. The most important achievements of horse genome analysis were well-developed linkage, radiation hybrid and cytogenetic genome maps including more than 2950 loci. These maps support comparative analysis of equine hereditary diseases. The present known gene mutations for five diseases in horses have human homologs. Studies on multifactorial diseases such as osteochondrosis and navicular bone disease and on fertility and temperament are underway. At the moment, the whole equine genome is sequenced as it has been done for the human genome and also for other animal genomes. Horse breeding will greatly benefit from identification of QTL for multifactorial traits and gene mutations for congenital anomalies, diseases and performance traits.

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“…Microsatellite markers UM-011, HTG-05 and HMS-42 mapping to equine chromosome 20 (ECA-20; Swinburne et al [23]) were genotyped in the 448 horses. While the UM-011 locus is placed within MHC class II (no recombination between UM-011 and DRB [23]), the other two microsatellites (HTG-05 and HMS-42), here used as flanking controls, are located proximal and distal on ECA-20.…”

Section: Genotyping Of Microsatellite Markersmentioning

confidence: 99%

“…While the UM-011 locus is placed within MHC class II (no recombination between UM-011 and DRB [23]), the other two microsatellites (HTG-05 and HMS-42), here used as flanking controls, are located proximal and distal on ECA-20. The three primer pairs (http://www.aht.org.uk/genetics/table2.html and http://locus.jouy.inrafr/cgi-bin/horsemap/) were pooled into a PCRmultiplex performed as described in Swinburne et al [23].…”

Section: Genotyping Of Microsatellite Markersmentioning

confidence: 99%

“…In order to further investigate whether the MHC gene region influences the allergen-specific IgE response in the horse, three microsatellite markers located on the same chromosome (ECA-20) as the equine MHC [23] were typed on 448 Lipizzan horses from six national studs. One of these markers, UM-011, is closely linked with the MHC class II DRB locus [23].…”

Section: Introductionmentioning

confidence: 99%

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Association between the MHC gene region and variation of serum IgE levels against specific mould allergens in the horse

et al. 2003

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-To investigate whether the equine major histocompatibility complex (MHC) gene region influences the production of mould-specific immunoglobulin E antibodies (IgE), alleles of the equine leukocyte antigen (ELA-A) locus and three microsatellite markers (UM-011, HTG-05 and HMS-42) located on the same chromosome as the equine MHC were determined in 448 Lipizzan horses. Statistical analyses based on composite models, showed significant associations of the ELA-A and UM-011 loci with IgE titres against the recombinant Aspergillus fumigatus 7 antigen (rAsp f 7). UM-011 was also significantly associated with IgE titres against the recombinant Aspergillus fumigatus 8 antigen (rAsp f 8). In addition to the loci mentioned above, the MHC class II DQA and DRA loci were determined in 76 Lipizzans from one stud. For IgE levels against rAsp f 7, the composite model showed the strongest association for DQA * Correspondence and reprints E-mail: eliane.marti@itz.unibe.ch S178 I. Curik et al.(P < 0.01) while for rAsp f 8 specific IgE levels, similarly to the results found with all 448 horses, the strongest association was found with UM-011 (P = 0.01), which is closely linked with the MHC class II DRB locus. These results suggest that the equine MHC gene region and possibly MHC class II loci, influence the specific IgE response in the horse. However, although the strongest associations were found with DQA and UM-011, this study did not distinguish if the observed effects were due to the MHC itself or to other tightly linked genes.horse / major histocompatibility complex / immunoglobulin E / specific mould allergen

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First Comprehensive Low-Density Horse Linkage Map Based on Two 3-Generation, Full-Sibling, Cross-Bred Horse Reference Families

Cited by 84 publications

References 27 publications

The First-Generation Whole-Genome Radiation Hybrid Map in the Horse Identifies Conserved Segments in Human and Mouse Genomes

The First-Generation Whole-Genome Radiation Hybrid Map in the Horse Identifies Conserved Segments in Human and Mouse Genomes

Mapping the horse genome and its impact on equine genomics for identification of genes for monogenic and complex traits – a review

Association between the MHC gene region and variation of serum IgE levels against specific mould allergens in the horse

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