First construction of interspecific backcross grouper and genome-wide identification of their genetic variants associated with early growth

Yang, Yang; Wang, Tong; Chen, Jingfang; Wu, Xi; Wu, Lina; Zhang, Weiwei; Luo, Jian; Xia, Jian‐Bai; Meng, Zining; Liu, Xiaochun

doi:10.1016/j.aquaculture.2021.737221

Cited by 12 publications

(3 citation statements)

References 87 publications

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“…Researchers have obtained several hybridization varieties using cryopreserved sperm, such as E. fuscoguttatus♀× E. lanceolatus♂ [5] and E. lanceolatus♂× E. akaara♀ [6]. The most successful one among them, brown-marbled grouper♀× giant grouper♂, has accounted for more than 70% of grouper market in China (over 19,000 tons in 2020), showing a promising promotion for the development of grouper industry [7][8][9][10]. However, during the process of sperm cryopreservation, oxidative damage caused by reactive oxygen species (ROS) is considered to be one of the main causes of the decline in sperm quality [11].…”

Section: Introductionmentioning

confidence: 99%

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm

Ruan,

Yang,

Hua

et al. 2024

Animals

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Sperm cryopreservation is a valuable tool for breeding, conservation, and genetic improvement in aquatic resources, while oxidative damage will cause a decline in sperm quality during this progress. Melatonin (MT), a natural antioxidant hormone, is used as an additive in sperm cryopreservation to reduce cellular damage from oxidative stress. Here, we aimed to investigate the effect of adding MT to the freezing medium in sperm cryopreservation of brown-marbled grouper (Epinephelus fuscoguttatus). Different concentrations of MT (0, 0.1, 0.25, and 0.5 mg/mL) were tested. We evaluated sperm motility, viability, apoptosis, mitochondrial membrane potential (MMP), and fertilization ability to assess the effects of MT supplementation. Our results demonstrated that the addition of MT to the extender improved the post-thaw motility, MMP, and fertilization ability of brown-marbled grouper sperm. The total motility, curvilinear velocity, straight linear velocity, and average path velocity in MT-treated groups (0.1 and 0.25 mg/mL) exhibited significantly higher values than that of the control group. A higher MMP (p < 0.05) was observed in the group treated with 0.25 mg/mL MT, suggesting that supplementation of MT in the extender might be able to protect mitochondrial membrane integrity effectively. Regarding fertilizing ability, 0.25 mg/mL MT yielded a significantly higher hatching rate than the control. An adverse effect was found with the concentration of MT up to 0.5 mg/mL, suggesting the possible toxicity of a high-dose addition. In this study, we optimized the sperm cryopreservation protocol of brown-marbled grouper, which might be valuable for sperm cryopreservation and sample commercialization of groupers and other fish.

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Section: Introductionmentioning

confidence: 99%

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm

Ruan,

Yang,

Hua

et al. 2024

Animals

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“…49-22.50 Myr, which are considered pivotal factors for the success of hybrid groupers [11]. An F2 segregation population of the Hulong hybrid grouper was applied to analyze the genetic basis of the growth advantage, and several genes involved in energy metabolism, development, stress tolerance, the immune response and visual perception were found to be associated with growth traits in the juvenile stage of the giant grouper [12].…”

Section: Introductionmentioning

confidence: 99%

Assembly of Genome and Resequencing Provide Insights into Genetic Differentiation between Parents of Hulong Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

Yang

Wang

et al. 2023

IJMS

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The Hulong hybrid grouper was bred from the brown-marbled grouper (Epinephelus fuscoguttatus) ♀ and the giant grouper (E. lanceolatus) ♂, combining the advantageous traits of both parents. Possessing an excellent performance, this hybrid’s cultivation promotes the development of the grouper industry. Its male parent, the giant grouper, possesses the fastest growth and the largest body size among all coral-reef-dwelling fish. This species is not only an economically important species in marine aquaculture, but it is also an ideal male parent in the interspecific crossing of grouper species. In the present study, a high-quality chromosome-level genome of the giant grouper was constructed with a total length of 1.06 Gb, consisting of 24 chromosomes and 69 scaffolds. To analyze the genetic differences between the parents of the Hulong hybrid grouper, the structural variations (SVs) between both parental genomes were detected, and a total of 46,643 SVs were obtained. High-quality SNPs were identified from resequencing data. There were significant differences between the two genomes, and the average FST reached 0.685. A total of 234 highly differentiated regions were detected with an FST > 0.9. The protein-coding genes involved in SVs and highly differentiated regions were significantly enriched in metabolic pathways, including fatty metabolism, carbohydrate metabolism, amino acid metabolism and the TCA cycle. These genes may be related to the differences in feeding preferences and the ability to digest carbohydrates between the two grouper species under natural conditions. In addition, protein-coding genes related to the cell cycle and p53-signaling pathway were also detected. These genes may play important roles in the regulation of body size and growth performance. This research provides genomic resources for further breeding works and evolutionary analyses.

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“…Because of its excessive nutritional value, tender meat, and delicious taste, its miles favoured in South and Southeast Asian markets. Due to incomplete reproductive isolation and the large diversity of grouper, traditional hybrid breeding has played an important role in the development of the grouper breeding industry (Yang et al 2021). Several of seeds grouper that have been successfully to produce in Indonesia are tiger grouper (Epinephelus fuscoguttatus), humpback grouper (Cromileptes altivelis), coral trouth (Plectropomus leopardus), and Hybrid grouper are cantang (E. fuscoguttatus × E. lanceolatus) (Sun et al 2016) and cantik (E. fuscoguttatus × E. polyphekadion) (Cao et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Production Performance and Financial Analysis of Three Segments Business to Support Availability Hybrid Grouper Seeds in Marine Culture

Astari¹,

Effendi²,

Budiardi³

et al. 2023

Preprint

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Important factors in grouper mariculture are continuous availability of seeds. Purpose of this research was to analyse production performance and financial analysis of three segments that support production of cantang hybrid grouper (macan/tiger, Epinephelus fuscoguttatus × kertang/giant, Epinephelus lanceolatus) and cantik hybrid grouper (macan/tiger, Epinephelus fuscoguttatus × batik/camouflage, Epinephelus microdon). Research was conducted for one year in 2022 three businesses, namely egg production, small-scale hatchery and nursery businesses. Egg production in a year for cantang hybrid grouper 97,300,000 eggs and cantik hybrid gouper of 17,800,000 eggs. Small-scale hatchery were harvested with average total length of ± 3.0 cm with SR 2.1–15.7% for cantang and 4.3–18.2% for cantik. Nursery were harvested with average total length of cantang seeds was 10.9 ± 0.3 cm with SR of 60.2–84.5% and seeds was 10.1 ± 0.3 cm with SR of 74.9–91.8%. Egg production obtained profit IDR 444,144,168/year, R/C ratio 1.67, and payback period 1.16 year. Egg production business in 5 years obtained NPV IDR 834,544,410, net B/C 2.63, and IRR 78.90%. Small-scale hatchery business profit IDR 414,508,032/year, R/C ratio 1.94, and payback period 1.40 year with NPV IDR 783,041,394, net B/C 2.35, and IRR 65.10%. Nursery business profit IDR 524,700,000/year, R/C ratio 1.73, and payback period 1.17 year with NPV IDR 1,097,840,171, net B/C 2.78, and IRR 79.30%. Sensitivity analysis shows that increases and decreases that occur in amount of production and selling prices have significant effect on NPV value. In addition, increase in profitability performance is associated with decrease in broodstock, eggs, seeds, feeds, or labor costs.

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First construction of interspecific backcross grouper and genome-wide identification of their genetic variants associated with early growth

Cited by 12 publications

References 87 publications

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm

Assembly of Genome and Resequencing Provide Insights into Genetic Differentiation between Parents of Hulong Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)

Production Performance and Financial Analysis of Three Segments Business to Support Availability Hybrid Grouper Seeds in Marine Culture

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