First cyclotide from Hybanthus (Violaceae)

Broussalis, Adriana M.; Göransson, Ulf; Coussio, J.; Ferraro, Graciela; Martino, Virginia; Claeson, Per

doi:10.1016/s0031-9422(01)00173-x

Cited by 81 publications

(76 citation statements)

References 13 publications

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“…The sequences of the new peptides, named Hyfl A-C, are summarized in Table 1. The peptide naming convention established for H. parviflorus is adopted in this work (Broussalis et al, 2001). Hyfl A is similar to the previously reported cyclotides circulin A and Hypa A (68 and 74% identity, respectively).…”

Section: Resultssupporting

confidence: 68%

“…Comparison of Hyfl A with the previously determined sequence of Hypa A from the Argentine H. parviflorus (Broussalis et al, 2001) indicates high sequence similarity. The other two new peptides displayed residues previously unseen in their respective loops.…”

Section: Discussionmentioning

confidence: 69%

“…Loop 6 is formed at the cyclization point of the linear precursor. References for the listed cyclotides are as follows: cycloviolin C (Hallock et al, 2000), varv A (Claeson et al, 1998), Hypa A (Broussalis et al, 2001), cyclopsychotride (Witherup et al, 1994), circulin A (Derua et al, 1996), palicourein (Bokesch et al, 2001), and kalata B1 (Saether et al, 1995). A ribbon diagram of the cyclotide framework based on the structure of kalata B1 (PDB ID 1NB1) with the cystine knot core rendered and numbering of loops is shown in the middle of the figure.…”

Section: Introductionmentioning

confidence: 99%

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A Continent of Plant Defense Peptide Diversity: Cyclotides in AustralianHybanthus(Violaceae)

et al. 2005

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Cyclotides are plant-derived miniproteins that have the unusual features of a head-to-tail cyclized peptide backbone and a knotted arrangement of disulfide bonds. It had been postulated that they might be an especially large family of host defense agents, but this had not yet been tested by field data on cyclotide variation in wild plant populations. In this study, we sampled Australian Hybanthus (Violaceae) to gain an insight into the level of variation within populations, within species, and between species. A wealth of cyclotide diversity was discovered: at least 246 new cyclotides are present in the 11 species sampled, and 26 novel sequences were characterized. A new approach to the discovery of cyclotide sequences was developed based on the identification of a conserved sequence within a signal sequence in cyclotide precursors. The number of cyclotides in the Violaceae is now estimated to be >9000. Cyclotide physicochemical profiles were shown to be a useful taxonomic feature that reflected species and their morphological relationships. The novel sequences provided substantial insight into the tolerance of the cystine knot framework in cyclotides to amino acid substitutions and will facilitate protein engineering applications of this framework.

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Section: Resultssupporting

confidence: 68%

Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

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A Continent of Plant Defense Peptide Diversity: Cyclotides in AustralianHybanthus(Violaceae)

et al. 2005

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“…The primary structure of vhr1 shows high sequence homology to cycloviolacin O1 , isolated from the aerial parts of V. odorata, including a rather unusual AES triad in loop 1. Only five cyclotides (vhr1, cycloviolacin O1 , vico A and B , and hypa A [Broussalis et al, 2001]) have been reported to contain an Ala residue in position 2 instead of the more common Gly residue. Interestingly, all AES mutants have been isolated from Violaceae plants, raising the question of whether this mutation is a Violaceae-specific phenomenon.…”

Section: Solution Structure Of Vhr1 and Comparison With Other Cyclotidesmentioning

confidence: 99%

Tissue-Specific Expression of Head-to-Tail Cyclized Miniproteins in Violaceae and Structure Determination of the Root CyclotideViola hederacearoot cyclotide1[W]

2004

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The plant cyclotides are a family of 28 to 37 amino acid miniproteins characterized by their head-to-tail cyclized peptide backbone and six absolutely conserved Cys residues arranged in a cystine knot motif: two disulfide bonds and the connecting backbone segments form a loop that is penetrated by the third disulfide bond. This knotted disulfide arrangement, together with the cyclic peptide backbone, renders the cyclotides extremely stable against enzymatic digest as well as thermal degradation, making them interesting targets for both pharmaceutical and agrochemical applications. We have examined the expression patterns of these fascinating peptides in various Viola species (Violaceae). All tissue types examined contained complex mixtures of cyclotides, with individual profiles differing significantly. We provide evidence for at least 57 novel cyclotides present in a single Viola species (Viola hederacea). Furthermore, we have isolated one cyclotide expressed only in underground parts of V. hederacea and characterized its primary and three-dimensional structure. We propose that cyclotides constitute a new family of plant defense peptides, which might constitute an even larger and, in their biological function, more diverse family than the well-known plant defensins.

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“…They are found in a variety of dicot plants, including the Cucurbitaceae (10), Rubiaceae (11), Violaceae (12,13), Fabaceae (9,14), and Solanaceae families (15). They are ribosomally synthesized as linear precursors, excised at the N and C termini of their mature cyclotide domains, and then head-to-tail ligated to produce mature cyclic peptides (4).…”

mentioning

confidence: 99%

Discovery of Linear Cyclotides in Monocot Plant Panicum laxum of Poaceae Family Provides New Insights into Evolution and Distribution of Cyclotides in Plants

Nguyen

Lian

Pang

et al. 2013

Journal of Biological Chemistry

113

112

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Background: Cyclotides are biologically active, plant-derived macrocyclic peptides. All cyclotides and their acyclic variants have been isolated from dicots. Results: We characterized nine novel linear cyclotides from monocot plant Panicum laxum. Conclusion: Our study provides the first evidence of linear cyclotides at the protein level in the Poaceae. Significance: Ancient linear cyclotide analogs may have existed before the divergence of dicots and monocots.

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First cyclotide from Hybanthus (Violaceae)

Cited by 81 publications

References 13 publications

A Continent of Plant Defense Peptide Diversity: Cyclotides in AustralianHybanthus(Violaceae)

A Continent of Plant Defense Peptide Diversity: Cyclotides in AustralianHybanthus(Violaceae)

Tissue-Specific Expression of Head-to-Tail Cyclized Miniproteins in Violaceae and Structure Determination of the Root CyclotideViola hederacearoot cyclotide1[W]

Discovery of Linear Cyclotides in Monocot Plant Panicum laxum of Poaceae Family Provides New Insights into Evolution and Distribution of Cyclotides in Plants

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