Two new species of Eustigmaeus Berlese (Acari: Stigmaeidae), E. incisus n. sp. and E. paraincisus n. sp. are described and illustrated from Türkiye. This paper presents compile checklist of the valid Eustigmaeus species. Eustigmaeus sphagneticolus Cooreman stat. nov., originally described as a subspecies of E. ottavii by Cooreman in 1943, is regarded as a separate species. Eustigmaeus aminiae (Nazari and Khanjani) n. comb.. and E. indiscretus (Dönel and Doğan) n. comb.. (from Ledermuelleriopsis Willmann) have a partially detached hysterosomal shield. Currently, we consider that these species belong more comfortably in Eustigmaeus than in Ledermuelleriopsis, where they are considered transitional species between the genera Eustigmaeus and Ledermuelleriopsis.