First detection and molecular analysis of SARS-CoV-2 from a naturally infected cat from Argentina

Fuentealba, Nadia Analía; Moré, Gastón; Bravi, María Emilia; Unzaga, Juan Manuel; Felice, Lorena De; Salina, Marcos; Viegas, Mariana; Jodar, Mercedes Soledad Nabaes; Valinotto, Laura; Rivero, Fernando; Lullo, David Di; Pecoraro, Marcelo Ricardo Ítalo; Panei, Carlos Javier

doi:10.1016/j.vetmic.2021.109179

Cited by 24 publications

(22 citation statements)

References 33 publications

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“…In most studies, the cycle threshold values determined in samples from infected animals were high. Although different methodologies are used in each work, the Cts associated with the detection of N, Orf1ab, and RdRP genes were higher than 30 ( 9 , 11 , 16 , 18 , 33 ).…”

Section: Discussionmentioning

confidence: 96%

“…The first report of reverse zoonosis, was a dog from Hong Kong ( 7 ). After that, other cases of domestic cats and dogs, wild felids (i.e., lion and tigers at the Bronx Zoo, USA), and minks becoming infected following exposure to infected owners were reported ( 6 , 8 , 9 ).…”

Section: Introductionmentioning

confidence: 99%

“…Since the beginning of the pandemic, domestic cats infected with SARS-CoV-2 have been identified in many countries including China ( 10 ), Belgium ( 11 ), Italy ( 12 ), UK ( 13 ), France ( 14 ), Spain ( 15 ), Brazil ( 16 ), USA ( 8 ), and Argentina ( 9 ). In addition, animal susceptibility experiments demonstrated that dogs, cats, ferrets, pigs, chickens, and ducks can be experimentally infected with SARS-CoV-2 ( 17 ) and that cats and ferrets are the most susceptible species.…”

Section: Introductionmentioning

confidence: 99%

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Anthropogenic Infection of Domestic Cats With SARS-CoV-2 Alpha Variant B.1.1.7 Lineage in Buenos Aires

Pécora

Malacari²,

Mozgovoj

et al. 2022

Front. Vet. Sci.

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SARS-CoV-2 reverse zoonosis, particularly to domestic animals, and the potential role of infected animals in perpetuating the spread of the virus is an issue of increasing concern. In this case report, we identified the natural infection of two cats by SARS-CoV-2, in Argentina, whose owner had been previously infected by SARS-CoV-2. Viral genetic material was detected in feline oropharyngeal (OP) and rectal (R) swab by RT-qPCR, and sequence analysis revealed that the virus infecting the owner and one cat were genetically similar. The alpha variant (B.1.1.7 lineage) was identified with a unique additional mutation, strongly suggesting human-to-cat route of transmission. This study reinforces the One Health concept and the importance of integrating human, animal, and environmental perspectives to promptly address relevant health issues.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anthropogenic Infection of Domestic Cats With SARS-CoV-2 Alpha Variant B.1.1.7 Lineage in Buenos Aires

Pécora

Malacari²,

Mozgovoj

et al. 2022

Front. Vet. Sci.

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“…As a consequence of the animals assessed massively in some countries, the prevalence was higher in Denmark (Larsen et al 2021 ). There is a lack of studies in Latin America, a region severely affected by the pandemic, except Mexico, Brazil, Argentina, and Chile (Calvet et al 2021 ), and now Argentina where a recent study detected the SARS-CoV-2 infection in 18 cats and 20 dogs from owners previously confirmed as COVID-19-positive, including genome sequencing, B.1.499, a lineage reported in different provinces of the country (Fuentealba et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 natural infection in animals: a systematic review of studies and case reports and series

Bonilla-Aldana

García-Barco²,

Jimenez-Diaz

et al. 2021

Veterinary Quarterly

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COVID-19 pandemic is essentially a zoonotic disease. In this context, early in 2020, transmission from humans to certain animals began reporting; the number of studies has grown since. To estimate the pooled prevalence of SARS-CoV-2 natural infection in animals and to determine differences in prevalence between countries, years, animal types and diagnostic methods (RT-PCR or serological tests). A systematic literature review with meta-analysis using eight databases. Observational studies were included but analyzed separately. We performed a random-effects model meta-analysis to calculate the pooled prevalence and 95% confidence interval (95% CI) for prevalence studies and case series. After the screening, 65 reports were selected for full-text assessment and included for qualitative and quantitative analyses. A total of 24 reports assessed SARS-CoV-2 infection by RT-PCR, combining a total of 321,785 animals, yielding a pooled prevalence of 12.3% (95% CI 11.6%–13.0%). Also, a total of 17 studies additionally assessed serological response against SARS-CoV-2, including nine by ELISA, four by PRTN, one by MIA, one by immunochromatography (rest, two studies, the method was not specified), combining a total of 5319 animals, yielding a pooled prevalence of 29.4% (95% CI 22.9%–35.9%). A considerable proportion of animals resulted infected by SARS-CoV-2, ranking minks among the highest value, followed by dogs and cats. Further studies in other animals are required to define the extent and importance of natural infection due to SARS-CoV-2. These findings have multiple implications for public human and animal health. One Health approach in this context is critical for prevention and control.

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“…This scenario has become evident with the SARS-CoV-2 pandemic ( SI Appendix , Table S1 ). Originally resulting from viral spillover into humans ( 4 , 5 ), likely from an animal reservoir, spillback into a wide range of companion and wild animals has occurred or been shown to be plausible ( 6 – 10 ), and an increasing number of studies have indicated a high frequency of human-to-animal SARS-CoV-2 spillback transmission ( 11 – 18 ). Given the short duration of viral shedding, serologic analyses present a more accurate characterization of actual animal exposures to SARS-CoV-2.…”

mentioning

confidence: 99%

SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection

Bashor

Gagne

Bosco‐Lauth

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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SARS-CoV-2 spillback from humans into domestic and wild animals has been well documented, and an accumulating number of studies illustrate that human-to-animal transmission is widespread in cats, mink, deer, and other species. Experimental inoculations of cats, mink, and ferrets have perpetuated transmission cycles. We sequenced full genomes of Vero cell–expanded SARS-CoV-2 inoculum and viruses recovered from cats (n = 6), dogs (n = 3), hamsters (n = 3), and a ferret (n = 1) following experimental exposure. Five nonsynonymous changes relative to the USA-WA1/2020 prototype strain were near fixation in the stock used for inoculation but had reverted to wild-type sequences at these sites in dogs, cats, and hamsters within 1- to 3-d postexposure. A total of 14 emergent variants (six in nonstructural genes, six in spike, and one each in orf8 and nucleocapsid) were detected in viruses recovered from animals. This included substitutions in spike residues H69, N501, and D614, which also vary in human lineages of concern. Even though a live virus was not cultured from dogs, substitutions in replicase genes were detected in amplified sequences. The rapid selection of SARS-CoV-2 variants in vitro and in vivo reveals residues with functional significance during host switching. These observations also illustrate the potential for spillback from animal hosts to accelerate the evolution of new viral lineages, findings of particular concern for dogs and cats living in households with COVID-19 patients. More generally, this glimpse into viral host switching reveals the unrealized rapidity and plasticity of viral evolution in experimental animal model systems.

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First detection and molecular analysis of SARS-CoV-2 from a naturally infected cat from Argentina

Cited by 24 publications

References 33 publications

Anthropogenic Infection of Domestic Cats With SARS-CoV-2 Alpha Variant B.1.1.7 Lineage in Buenos Aires

Anthropogenic Infection of Domestic Cats With SARS-CoV-2 Alpha Variant B.1.1.7 Lineage in Buenos Aires

SARS-CoV-2 natural infection in animals: a systematic review of studies and case reports and series

SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection

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