First detection of bluetongue virus serotype 14 in Poland

Orłowska, Anna; Trębas, Paweł; Smreczak, Marcin; Marzec, Anna; Zmudzinski, J.F.

doi:10.1007/s00705-016-2857-0

Cited by 22 publications

(29 citation statements)

References 9 publications

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“…BTV‐14 infection was confirmed in four European bison from Białowieża Primeval Forest and in cattle in the neighbouring region of Sokółka by real‐time RT‐PCR at the Pirbright Institute (Krzysiak, unpublished data; Pirbright Institute, ). The same homologous to South African vaccine BTV‐14 strain virus has been observed circulating in this area between 2011 and 2014 (Orłowska et al., ). The virus was also isolated in KC4 ( C. sonorensis ‐derived cell line) and BHK1 (baby hamster kidney) cells (http://www.reoviridae.org/dsrna_virus_proteins/reoid/btv-14.htm).…”

Section: Discussionmentioning

confidence: 65%

Serological Study of Exposure to Selected Arthropod-Borne Pathogens in European Bison (Bison bonasus) in Poland

Krzysiak

Iwaniak

Kęsik-Maliszewska

et al. 2016

Transbound Emerg Dis

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Bison bonasus is an indigenous species of Central and Eastern Europe with the largest wild population inhabiting Białowieża Primeval Forest; however, free-living and captive European bison are reared in many countries around the world. Despite that the European bison was rescued from the extinction after the First World War, it remains as endangered species. Changing environment as well as human activity may have contributed to the observed increase of the risk of the emergence and re-emergence of pathogens. The aim of the survey was to establish the distribution of four pathogens transmitted by arthropods including three arboviruses [Bluetongue disease virus (BTV), Epizootic haemorrhagic disease virus (EHDV) and Schmallenberg virus (SBV)] and a bacteria (Francisella tularensis) in the main populations of European bison in Poland. A total of 251 European bison originating from eight main populations were included in the study and sampled between February 2011 and December 2014. Serum samples originated from chemically immobilized, eliminated or dead by natural causes animals. Additionally, 65 cervids from Białowieża Forest were tested to compare the seroprevalences of other ruminants inhabiting the same environment. The antibodies to SBV and BTV were found in 76.1% and 24.7% of European bison, respectively. In autumn 2012, simultaneous emergence of SBV and BTV in European bison was observed; however, while SBV has spread in all populations scattered around the country, BTV infections were observed only in the north-eastern part of Poland, where BTV cases have been previously reported in domestic ruminants. European bison age was found to be the only significant risk factor for SBV and BTV seroprevalences; however, this association was connected to the animal size, rather than to the length of exposure. None of the animals tested positive for antibodies against EHDV or F. tularensis. SBV exposure rate of cervids was much lower (35.4%) than in European bison, while BTV seroprevalence was comparable in both groups.

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Section: Discussionmentioning

confidence: 65%

Serological Study of Exposure to Selected Arthropod-Borne Pathogens in European Bison (Bison bonasus) in Poland

Krzysiak

Iwaniak

Kęsik-Maliszewska

et al. 2016

Transbound Emerg Dis

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“…Sequencing of the VP2 (Seg-2) and NS1 (Seg-5) genes resulted in the Russian BTV-14 strain being grouped with a vaccinelike BTV-14 strain used in African countries (Figure 2). In addition to these findings, a related BTV-14 strain was also documented in Poland in animals near the Polish-Belarusian and Polish-Lithuanian borders in 2012 (47). Notably, Russian strain G244/11, strain SPA2012/01 detected in cattle in Spain imported from Lithuania, and Polish strain POL2012/01 shared approximately 99.9% identity based on the VP2 gene.…”

Section: Discussionmentioning

confidence: 67%

“…The serendipitous detection of BTV-14 in the cattle in the Smolensk region in 2011-2012 represents the first historical incidence of BTV in the European part of Russia in which antibodies and live viruses were identified in local livestock. The circulation of the live virus was confirmed by detection of BTV-14 in the neighboring Smolensk and Kaluga regions in 2012 (Figure 1, Table 2), as well as in Poland in 2012 and 2014 (47).…”

Section: Discussionmentioning

confidence: 76%

Identification and Characterization of Bluetongue Virus Serotype 14 in Russia

et al. 2020

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“…As the virus has not been isolated from any of the PCR-positive samples, it can be concluded that the BTV-positive animals were probably in the late stages of infection, when animals remain PCR-positive, but are unlikely to be infective to midges. Recently, the first cases of BTV detected in native cattle from Poland have been described (33). In the National Reference Laboratory for Bluetongue, BTV serotype 14 was detected by type-specific rRT--PCR in animals reared near the Polish-Belarusian and Polish-Lithuanian borders.…”

mentioning

confidence: 99%

“…In the National Reference Laboratory for Bluetongue, BTV serotype 14 was detected by type-specific rRT--PCR in animals reared near the Polish-Belarusian and Polish-Lithuanian borders. It was suggested that the most probable route of BTV introduction into Poland was transmission through Culicoides midges (33).…”

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confidence: 99%

Molecular technologies for detection and typing of bluetongue virus

Niedbalski

2017

Medycyna Weterynaryjna

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Rapid and accurate diagnosis plays an important role in the implementation of effective measures to control the spread of disease. Historically, the laboratory diagnosis and typing of BTV were carried out by various serological and virological methods, including virus neutralization (VN) assay, ELISA, as well as virus isolation (VI) in cell cultures or in embryonated chicken eggs. At present, various molecular techniques to detect BTV genome are increasingly used as primary diagnostic tools for the serotyping and epidemiological investigations of BTV. Initially, the viral RNA was detected by simple nucleic acid hybridization technologies. Then, conventional RT-PCR assays were developed and evaluated for the detection of BTV serotypes based on nucleotide sequences of different genome segments. Although RT-PCR, with its increased sensitivity, has advantages over hybridization, it is almost impossible to quantify accurately by regular and multiplex PCR procedures, and regular PCR may produce false positive results. Over the recent years, a number of real-time RT-PCR (rRT-PCR) methods have been described. The rRT-PCR offers certain advantages over conventional RT-PCR assay, as it is more rapid, sensitive, and can provide quantitative as well as qualitative genetic information. It does not use agarose gel electrophoresis, decreases the risk of contamination because it is run within an enclosed tube, and is suitable for large-scale testing and automation. The target amplicon is usually smaller, reducing the potential for problems caused by target degradation. Loop-mediated isothermal amplification (LAMP), a novel rapid, accurate and cost effective gene amplification method, is an autocycling and strand displacement DNA synthesis method. LAMP assays have been applied as a method of detecting a variety of animal pathogens, including BTV. RT- LAMP assay can be a valuable tool complementing the routine laboratory diagnosis of BTV.

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First detection of bluetongue virus serotype 14 in Poland

Cited by 22 publications

References 9 publications

Serological Study of Exposure to Selected Arthropod-Borne Pathogens in European Bison (Bison bonasus) in Poland

Serological Study of Exposure to Selected Arthropod-Borne Pathogens in European Bison (Bison bonasus) in Poland

Identification and Characterization of Bluetongue Virus Serotype 14 in Russia

Molecular technologies for detection and typing of bluetongue virus

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