First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR

Faria, Ana; Paiva-Cardoso, Maria das Neves; Nunes, Mónica; Vale-Gonçalves, Hélia; Veloso, Octávia; Coelho, Catarina; Cabral, João Alexandre; Vieira-Pinto, Madalena; Vieira, Maria Luísa

doi:10.1007/s10393-014-0973-4

Cited by 15 publications

(17 citation statements)

References 25 publications

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“…is currently considered the most common tick-borne pathogen in the northern hemisphere and it has been frequently detected in ticks and wild animals in Italy [17][18][19][20][21][22]. Several vertebrates are involved in the cycle of this agent, but the role of wild boars is currently not defined [7]. Our results are strongly in agreement with those obtained in a recent molecular investigation which found B. burgdorferi s.l.…”

Section: Resultssupporting

confidence: 91%

“…Our results are strongly in agreement with those obtained in a recent molecular investigation which found B. burgdorferi s.l. in wild boars blood samples with a 3.3% prevalence [7]. A serological survey carried out in Czech…”

Section: Resultsmentioning

confidence: 99%

“…Even though serological and molecular investigations have been carried out to estimate the spreading of tick-borne pathogens among wildlife, data about the epidemiological role of wild boars (Sus scrofa) in these infections are very scant [2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Ebani¹,

Ebani²,

Bertelloni³

et al. 2017

APJTD

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

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Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Ebani¹,

Ebani²,

Bertelloni³

et al. 2017

APJTD

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“…l . ), even though the presence of DNA specific for these bacteria have been sporadically detected in their tissues [57–60]. It is assumed that due to complement-mediated killing, the presence of B. burgdorferi ( s .…”

Section: Introductionmentioning

confidence: 99%

Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia

et al. 2018

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BackgroundFree-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central Europe and many regions around the world. Tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western Slovakia. DNA isolated from these samples was screened for the presence of tick-borne microorganisms by PCR-based methods.ResultsTicks were found to infest all examined ungulate species. The principal infesting tick was Ixodes ricinus, identified on 90.4% of wildlife, and included all developmental stages. Larvae and nymphs of Haemaphysalis concinna were feeding on 9.6% of wildlife. Two specimens of Dermacentor reticulatus were also identified. Ungulates were positive for A. phagocytophilum and Theileria spp. Anaplasma phagocytophilum was found to infect 96.1% of cervids, 88.9% of mouflon, and 28.2% of wild boar, whereas Theileria spp. was detected only in cervids (94.6%). Importantly, a high rate of cervids (89%) showed mixed infections with both these microorganisms. In addition to A. phagocytophilum and Theileria spp., Rickettsia helvetica, R. monacensis, unidentified Rickettsia sp., Coxiella burnetii, “Candidatus Neoehrlichia mikurensis”, Borrelia burgdorferi (s.l.) and Babesia venatorum were identified in engorged I. ricinus. Furthermore, A. phagocytophilum, Babesia spp. and Theileria spp. were detected in engorged H. concinna. Analysis of 16S rRNA and groEL gene sequences revealed the presence of five and two A. phagocytophilum variants, respectively, among which sequences identified in wild boar showed identity to the sequence of the causative agent of human granulocytic anaplasmosis (HGA). Phylogenetic analysis of Theileria 18S rRNA gene sequences amplified from cervids and engorged I. ricinus ticks segregated jointly with sequences of T. capreoli isolates into a moderately supported monophyletic clade.ConclusionsThe findings indicate that free-living ungulates are reservoirs for A. phagocytophilum and Theileria spp. and engorged ixodid ticks attached to ungulates are good sentinels for the presence of agents of public and veterinary concern. Further analyses of the A. phagocytophilum genetic variants and Theileria species and their associations with vector ticks and free-living ungulates are required.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3068-1) contains supplementary material, which is available to authorized users.

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“…Among the diseases caused by tick-borne pathogens, Lyme borreliosis caused by spirochetes of the Borrelia burgdorferi ( sensu lato ) ( s.l. ) complex is currently the most common tick-borne disease in Europe [ 17 ]. In Portugal, its notification in humans is mandatory, but the disease is clearly underdiagnosed and underreported [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal

et al. 2016

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BackgroundWildlife can act as reservoir of different tick-borne pathogens, such as bacteria, parasites and viruses. The aim of the present study was to assess the presence of tick-borne bacteria and protozoa with veterinary and zoonotic importance in cervids and wild boars from the Centre and South of Portugal.MethodsOne hundred and forty one blood samples from free-ranging ungulates including 73 red deer (Cervus elaphus), 65 wild boars (Sus scrofa) and three fallow deer (Dama dama) were tested for the presence of Anaplasma marginale/A. ovis, A. phagocytophilum, Anaplasma/Ehrlichia spp., Babesia/Theileria spp., Borrelia burgdorferi (sensu lato) (s.l.), and Rickettsia spp. DNA by PCR.ResultsAnaplasma spp. DNA was detected in 33 (43.4 %) cervids (31 red deer and two fallow deer) and in two (3.1 %) wild boars while Theileria spp. were found in 34 (44.7 %) cervids (32 red deer and two fallow deer) and in three (4.6 %) wild boar blood samples. Sequence analysis of msp4 sequences identified A. marginale, A. ovis, while the analysis of rDNA sequence data disclosed the presence of A. platys and A. phagocytophilum and T. capreoli and Theileria sp. OT3. Anaplasma spp./Theileria spp. mixed infections were found in 17 cervids (22.4 %) and in two wild boars (3.1 %). All samples were negative for Babesia sp., B. burgdorferi (s.l.), Ehrlichia sp. or Rickettsia sp.ConclusionsThis is the first detection of Anaplasma marginale, A. ovis, A. phagocytophilum, A. platys, Theileria capreoli and Theileria sp. OT3 in cervids and wild boars from Portugal. Further studies concerning the potential pathogenicity of the different species of Anaplasma and Theileria infecting wild ungulates, the identification of their vector range, and their putative infectivity to domestic livestock and humans should be undertaken.

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First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR

Cited by 15 publications

References 25 publications

Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Zoonotic tick-borne bacteria among wild boars (Sus scrofa) in Central Italy

Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia

Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal

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