First detection of canine parvovirus type 2c in South America

Isolation and characterization of canine parvovirus type 2c circulating in Uruguay

“…It was previously reported that this is the predominant variant in Uruguay (PEREZ et al, 2007), Argentina (CALDERON et al, 2009) andBrazil (STRECK et al, 2009;CASTRO et al, 2010). To our knowledge the isolation in cell culture of CPV-2c strain was not described in the neighboiring countries, therefore this research is the first report in Uruguay and in the region about CPV-2c isolation in A-72 canine cell line.…”

“…This is a mutant of CPV-2b which involved the substitution Aspartate (Asp) 426 Glutamate (Glu) occurring in a main neutralizing epitope of the capsid (BUONAVOGLIA et al, 2001). Since its has been identified, CPV-2c has been spread over local canine population and has also been found in other European countries (DECARO et al, 2007), in Asia (NAKAMURA et al, 2004;NANDI et al, 2009), in Africa (TOUIHRI et al, 2009) and in the America; USA (HONG et al, 2007;KAPIL et al, 2007), Argentina (CALDERON et al, 2009), Brazil (CASTRO et al, 2010 and Uruguay (PEREZ et al, 2007 The CPV variants differ in amino acid changes occurring at residue 426 of the capsid protein, with types CPV-2a, CPV-2b, CPV-2c displaying amino acids Asparagine (Asn), Asp and Glu, respectively (BUONAVOGLIA et al, 2001).…”

“…Only one study reports the complete protection by the original CPV type 2 based vaccines against challenge with any of the CPV circulating strains (SPIBEY et al, 2008). However, there are several researches that reports vaccine failure when the challenge virus is the CPV type 2c (DECARO et al, 2007;PEREZ et al, 2007;DECARO et al, 2008;CALDERON et al, 2009). It is important to note that most current cases of canine parvovirosis are related to the virulent strain CPV-2c in Argentina (CALDERON et al, 2009), Uruguay (PEREZ et al, 2007) and Brazil (STRECK et al, 2009).…”

“…However, there are several researches that reports vaccine failure when the challenge virus is the CPV type 2c (DECARO et al, 2007;PEREZ et al, 2007;DECARO et al, 2008;CALDERON et al, 2009). It is important to note that most current cases of canine parvovirosis are related to the virulent strain CPV-2c in Argentina (CALDERON et al, 2009), Uruguay (PEREZ et al, 2007) and Brazil (STRECK et al, 2009). All these cases take place mainly in previously immunized animals whose vaccination probably failed, and CPV-2c strain would not be neutralized by vaccine strain CPV-2.…”

“…Despite CPV-2c was detected causing recent cases of canine parvovirosis in South America by molecular characterization (PEREZ et al, 2007;CALDERON et al, 2009;STRECK et al, 2009), the viable infectious particle was not isolated yet. The present study reports the first isolation in cell culture of a CPV-2c strain in Uruguay.…”