First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of cutaneous leishmaniasis, South of Iran

Rassi, Yavar; Hadi, Karami; Reza, Abai Mohammad; Mohebali, Mehdi; Hasan, Bakshi; Ali, Oshaghi Mohammad; Rafizadeh, Sayena; Habib, Bagherpoor Hagigi; Abodolrahim, Hosseini; Manuchehr, Gholami

doi:10.1016/s2221-1691(13)60163-x

Cited by 23 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported in Portugal [ 48 ], the presence of one pool of S. minuta infected with L. infantum , in our view, is not enough to conclusively incriminate this sand fly species as a vector. As reported in Greece and in Iran [ 49 , 50 ], the presence of L. infantum DNA in field-collected P. papatasi does not incriminate this sand fly species as a vector of ZVL. The presence of L. infantum DNA in S. minuta and P. papatasi could be explained by recent feedings on infected animals resulting in parasite DNA remnants after blood digestion.…”

Section: Discussionmentioning

confidence: 56%

Changes of Sand Fly Populations and Leishmania infantum Infection Rates in an Irrigated Village Located in Arid Central Tunisia

Barhoumi

Cherni

Derbali

et al. 2016

IJERPH

View full text Add to dashboard Cite

The current spread of zoonotic visceral leishmaniasis (ZVL) throughout arid areas of Central Tunisia is a major public health concern. The main objective of this study is to investigate whether the development of irrigation in arid bio-geographical areas in Central Tunisia have led to the establishment of a stable cycle involving sand flies of the subgenus Larroussius and Leishmania infantum, and subsequently to the emergence of ZVL. Sand flies were collected from the village of Saddaguia, a highly irrigated zone located within an arid bio-geographical area of Central Tunisia by using modified Centers for Diseases Control (CDC) light traps. Morphological keys were used to identify sand flies. Collected sand flies were pooled with up to 30 specimens per pool according to date and tested by nested Polymerase Chain Reaction (PCR) DNA sequencing from positive pools was used to identify Leishmania spp. A total of 4915 sand flies (2422 females and 2493 males) were collected from Saddaguia in September and in October 2014. Morphological identification confirmed sand flies of the subgenus Larroussius to be predominant. PCR analysis followed by DNA sequencing indicated that 15 pools were infected with L. infantum yielding an overall infection rate of 0.6%. The majority of the infected pools were of sand fly species belonging to subgenus Larroussius. Intense irrigation applied to the arid bio-geographical areas in Central Tunisia is at the origin of the development of an environment capable of sustaining important populations of sand flies of the subgenus Larroussius. This has led to the establishment of stable transmission cycles of L. infantum and subsequently to the emergence of ZVL.

show abstract

Section: Discussionmentioning

confidence: 56%

Changes of Sand Fly Populations and Leishmania infantum Infection Rates in an Irrigated Village Located in Arid Central Tunisia

Barhoumi

Cherni

Derbali

et al. 2016

IJERPH

View full text Add to dashboard Cite

show abstract

“…The vectorial role of P. ariasi has been shown in northern Morocco [40], while the epidemiological roles of atypical morph of P. perniciosus and P. longicuspis are not yet elucidated. Furthermore, P. papatasi and some species of the genus Sergentomyia, largely spread throughout Morocco and our study area, might also be able to transmit L. infantum [41,42]. Hence, it is essential to identify the still unknown sand fly species involved in the CanL transmission in these southern regions.…”

Section: Discussionmentioning

confidence: 97%

Epidemiological Investigation of Canine Leishmaniasis in Southern Morocco

Boussaa

Kasbari

Mzabi³

et al. 2014

Advances in Epidemiology

View full text Add to dashboard Cite

Dogs are the major reservoir ofLeishmania infantum, the causative agent of human and canine visceral leishmaniasis in the Mediterranean basin. In Morocco, canine leishmaniasis (CanL) is usually believed to be widespread mainly, if not only, in the northern regions and few data are available about the situation in southern parts of the country. Here, we report the results of a preliminary, clinical, and serological study carried out in 2004–2007, in four provinces of southern Morocco. Serological analyses were processed using two different Elisa techniques, a homemade Elisa test and IDVET commercial kit, and confirmed by two different western blot (WB) tests, homemade and LDBIO commercial kits. We highlighted the presence of CanL infection in southern regions, known until then as free of the disease: 19.8% (48/243) of examined dogs displayed clinical signs compatible with CanL and the seroprevalence was particularly high, respectively, 81.8% and 87.8% by Elisa and western blot tests. Our current developed and validated homemade (Elisa and WB) tools will be cost-effective and useful for next large-scale epidemiological studies on Moroccan leishmaniasis animal reservoir.

show abstract

“…Surprisingly, In Zaghouan, L. infantum DNA was detected in an engorged P. papatasi specimen. Such observation, although rare, has already been reported in Greece ( Aransay et al, 1933-1938 ), Iran ( Yavar et al, 2013 ), and recently in Italy ( Latrofa et al, 2018 ). However, L. infantum does complete its developmental life cycle in P. papatasi as it is eliminated after blood digestion and defecation ( Pimenta et al, 1994 ).…”

Section: Discussionmentioning

confidence: 73%

Investigation of natural infection of Phlebotomine (Diptera: Psychodidae) by Leishmania in Tunisian endemic regions

Chaouch

Chaabane

Ayari

et al. 2021

Parasite Epidemiology and Control

View full text Add to dashboard Cite

Leishmaniases are caused by protozoan parasites of the genus Leishmania transmitted by females blood-feeding phlebotomine insects (Diptera: Psychodidae). In Tunisia, cutaneous and visceral leishmaniases are of public health concern. In Tunisia, 17 species of phlebotomine sand flies are described. Here we investigate natural infection in Tunisian mixed foci regions of leishmaniases. We trap female sandflies during the Leishmania transmission season in the country's central-eastern and northern parts. We investigate Leishmania infection using PCR-RFLP targeting the ITS1 ribosomal DNA, followed by enzymatic digestion with Hae III; then, we identify sand flies using molecular methodologies. We confirm the presence of Phlebotomus papatasi and Phlebotomus perniciosus infected by L. major and L. infantum parasites in Tunisia.

show abstract

First detection of Leishmania infantum DNA in wild caught Phlebotomus papatasi in endemic focus of cutaneous leishmaniasis, South of Iran

Cited by 23 publications

References 17 publications

Changes of Sand Fly Populations and Leishmania infantum Infection Rates in an Irrigated Village Located in Arid Central Tunisia

Changes of Sand Fly Populations and Leishmania infantum Infection Rates in an Irrigated Village Located in Arid Central Tunisia

Epidemiological Investigation of Canine Leishmaniasis in Southern Morocco

Investigation of natural infection of Phlebotomine (Diptera: Psychodidae) by Leishmania in Tunisian endemic regions

Contact Info

Product

Resources

About