First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran

Nikkhahi, Farhad; Robatjazi, Saeedeh; Niazadeh, Mojtaba; Javadi, Amir; Shahbazi, Gholamhassan; Aris, Parisa; Marashi, Seyed Mahmoud Amin; Emam, Nasrin

doi:10.1016/j.nmni.2021.100862

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…The MIC results showed that none of the isolates, even E. coli O25-B2-ST131 strains, were resistant to colistin, and colistin was the most effective antibiotic against E. coli isolates from UTI. Although a small number of colistin-resistant E. coli ST131 strains have been isolated from different samples in studies, colistin has still been identified as the most effective antibiotic in previous studies [2,51].…”

Section: Discussionmentioning

confidence: 99%

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

Shahbazi

Salmanzadeh-Ahrabi²,

Aslani³

et al. 2023

BMC Pediatr

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Background Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered as multidrug-resistant and hypervirulent organism. There is lack of data about involvement of this pathogen in the children’s infection. In this study, the prevalence, and clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 compared with non-O25-B2-ST131 isolates were investigated in children with urinary tract infection in Tehran, Iran. Methods The E. coli isolates from urine samples were identified using conventional microbiological methods. Characterization of E. coli O25-B2-ST131 clone, antibiotic susceptibility, biofilm formation, ESBLs phenotype and genotype, serum resistance, hemolysis, hydrophobicity, and formation of curli fimbriae were done using conventional microbiological and molecular methods. Clonality of the isolates was done by rep-PCR typing. Results Among 120 E. coli isolates, the highest and lowest antibiotic resistance was detected against ampicillin (92, 76.6%) and imipenem 5, (4.1%), respectively. Sixty-eight (56.6%) isolates were ESBL-producing and 58 (48.3%) isolates were considered as multi-drug resistance (MDR). The prevalence of ESBL-producing and MDR isolates in O25-B2-ST131 strains was higher compared with the non-O25-B2-ST131 strains (p value < 0.05). O25-B2-ST131 strains showed significant correlation with serum resistance and biofilm formation. Amongst the resistance and virulence genes, the prevalence of iucD, kpsMTII, cnf1, vat, blaCTX-M-15, and blaSHV were significantly higher among O25-B2-ST131 isolates in comparison with non-O25-B2-ST131 isolates (p value < 0.05). Considering a ≥ 80% homology cut-off, fifteen different clusters of the isolates were shown with the same rep-PCR pattern. Conclusions Our results confirmed the involvement of MDR-ESBLs producing E. coli strain O25-B2-ST131 in the occurrence of UTIs among children. Source tracking and control measures seem to be necessary for containment of the spread of hypervirulent and resistance variants in children.

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Section: Discussionmentioning

confidence: 99%

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

Shahbazi

Salmanzadeh-Ahrabi²,

Aslani³

et al. 2023

BMC Pediatr

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“…Recently, a study was conducted in Iran, and for the first time, mcr-1 was detedcted in E. coli isolates rom livestock and wastewater (12). In a recent study, 37 (10.8%) colistin-resistant E. coli isolates were detected (MIC values >2 mg/L) and 36 fingerprint patterns were identified in colistin-resistant E. coli using ERIC-PCR (13)(Table 1).…”

Section: Iranmentioning

confidence: 99%

A Review of colistin-resistant Escherichia coli isolates in Middle East: Mechanisms, epidemiology, and dissemination from different origins in human, animal, food, and soil

2023

ARI

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Escherichia coli is a normal gut inhabitant, that is able to cause various diseases such as enteric, urinary tract, cystitis, and systemic infections in humans and animals. The alarming increase in the prevalence of profiles for the extended-spectrum β-lactamase producing and carbapenemase-producing Escherichia coli isolates is a serious problem all over the world. Colistin is well known as a last-resort antibiotic for the treatment of gram-negative bacterial infections. Inappropriate use of colistin and other classes of antibiotics in combination with poor infection control especially in developing countries could lead to serious complications in public health settings. The global increase of colistin resistance has been reported in many parts of the world, as well as in the Middle East region. Colistin is used to treat infections caused by extensively drug-resistant gram-negative bacteria. The reliable epidemiological data of the colistin-resistant in E. coli isolates is very scarce, however, information for colistin-resistant E. coli from Asia, the world's largest, most populous, and most diverse continent, is generally limited in comparison to Europe and the United States of America. The data of this review article were collected from the respective articles in connection with isolated colistin-resistant Escherichia coli (E. coli) isolates from humans, animals, and food-producing animals sections. In the Middle east, colistin-resistant E. coli isolates has been reported from Turkey, Egypt, Saudi Arabia, Algeria, Iran, Iraq, Bahrain, Qatar, Oman, Kuwait, Israel, and Lebanon between 2010 and 2022. While colistin resistance is most frequently observed in E. coli isolates, data indicated that the mcr genes have been found to be the most common genes associated with colistin resistance in E. coli isolates, in comparison with mutations in the pmrAsB, phoQ, and mgrB genes.

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“…[ 7 ] Numerous risk factors have been enumerated to be linked with the incidence of gastrointestinal cancer, including red meat, alcohol, obesity, age, smoking, sex, and even infectious diseases like helicobacter pillory, and viral diseases. [ 6 7 8 9 10 11 12 13 ]…”

Section: Introductionmentioning

confidence: 99%

The Evaluation of HERV-K np9, rec, gag Expression in Isolated Human Peripheral Blood Mononuclear Cell (PBMC) of Gastric and Colon Cancer

Faghihloo

Tavakolian

Iranshahi

2023

Advanced Biomedical Research

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Background: In the current age of diagnostic approaches in cancer, countless efforts have been allocated to identify novel and efficient biomarkers to detect cancer in its early stages. We focused on evaluating the correlation between the progression of gastrointestinal cancer, a leading cause of cancer death worldwide, and human endogenous retrovirus (HERV). Materials and Methods: In this study, we conducted a study on the peripheral blood mononuclear cells (PBMC) gathered from gastric and colon cancer patients. We focused on HERV-K rec, np9, gag expression analysis by quantitative real-time PCR, after extraction of RNA and synthesizing cDNA. Results: Unlike np9 whose expression increased significantly in the colon and gastric cancers, the mRNA level of the rec gene declined in both cancers. Moreover, our data illustrated that the over-expression of the gag gene was only observed in colon cancerous cells rather than gastric malignancy. Conclusions: Overall, given the correlation between the expression level of HERV-associated genes and gastrointestinal cancer, our study suggests that these genes could be considered beneficial markers for cancer diagnosis. However, researchers should conduct studies in future articles on whether these genes can be employed as biomarkers in gastrointestinal cancer.

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First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran

Cited by 9 publications

References 19 publications

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

A Review of colistin-resistant Escherichia coli isolates in Middle East: Mechanisms, epidemiology, and dissemination from different origins in human, animal, food, and soil

The Evaluation of HERV-K np9, rec, gag Expression in Isolated Human Peripheral Blood Mononuclear Cell (PBMC) of Gastric and Colon Cancer

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