First evidence of cytotoxic effects of human protozoan parasites on zebra mussel (<i>Dreissena polymorpha</i>) haemocytes

Guernic, Antoine Le; Geffard, Alain; Rioult, Damien; Bonnard, Isabelle; Foll, Frank Le; Ladeiro, Mélissa Palos

doi:10.1111/1758-2229.12720

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…These immune functions are accompanied by an overproduction of NO, which can be produced against microorganisms [71]. Not observed in previous studies [26,70], oocysts of C. parvum induced hemocyte necrosis. Although the number of activated caspases 3/7 did not differ among the condition of C. parvum exposure, this protozoan induced gene expression of these enzymes.…”

Section: Different Protozoa Different Interactions?mentioning

confidence: 76%

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Cellular and molecular complementary immune stress markers for the model species Dreissena polymorpha

Guernic

Geffard

Rioult

et al. 2020

Fish & Shellfish Immunology

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Section: Different Protozoa Different Interactions?mentioning

confidence: 76%

“…The T. gondii oocyst being about twice the size of that of C. parvum, it seems unlikely that it can be ingested. Nevertheless, immune cells of zebra mussels can aggregate around the oocysts to realize the phenomenon of encapsulation [70].…”

Section: Different Protozoa Different Interactions?mentioning

confidence: 99%

Cellular and molecular complementary immune stress markers for the model species Dreissena polymorpha

Guernic

Geffard

Rioult

et al. 2020

Fish & Shellfish Immunology

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“…gondii and G . duodenalis (oo)cysts were encapsulated in haemocyte aggregates because of their larger size (Le Guernic et al, 2019). Moreover, decreases in haemocyte viability and phagocytic parameters were observed after exposure of M .…”

Section: Discussionmentioning

confidence: 99%

Blue mussel ( Mytilus edulis )—A bioindicator of marine water contamination by protozoa: Laboratory and in situ approaches

Bigot-Clivot

Carbona

Cazeaux

et al. 2021

J of Applied Microbiology

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Aims:The protozoan parasites Cryptosporidium spp., Giardia duodenalis and Toxoplasma gondii are identified as public health priorities and are present in a wide variety of environments including the marine ecosystem. The objective of this study was to demonstrate that the marine bivalve blue mussel (Mytilus edulis) can be used as a tool to monitor the contamination of marine waters by the three protozoa over time. Methods and Results:In order to achieve a proof of concept, mussels were exposed to three concentrations of G. duodenalis cysts and Cryptosporidium parvum/T. gondii oocysts for 21 days, followed by 21 days of depuration in clear water. Then, natural contamination by these protozoa was sought for in wild marine blue mussels along the northwest coast of France to validate their relevance as bioindicators in the field. Our results highlighted that: (a) blue mussels bioaccumulated the parasites for 21 days, according to the conditions of exposure, and parasites could still be detected during the depuration period (until 21 days); (b) the percentage of protozoa-positive M. edulis varied under the degree of protozoan contamination in water; (c) mussel samples from eight out of nine in situ sites were positive for at least one of the protozoa. Conclusions:The blue mussel M. edulis can bioaccumulate protozoan parasites over long time periods, according to the degree of contamination of waters they are inhabiting, and can highlight recent but also past contaminations (at least 21 days). Significance and Impact of the Study: Mytilus edulis is a relevant bioaccumulators of protozoan (oo)cysts in laboratory and field conditions, hence its potential use for monitoring parasite contamination in marine waters.

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“…2019) while T. gondii lead to haemocyte aggregation suggesting potential encapsulation (Le Guernic et al . 2018). Oocysts have been detected in zebra mussel haemolymph suggesting that haemocyte–oocyst interactions may be possible in vivo (Graczyk et al .…”

Section: Discussionmentioning

confidence: 99%

Survival and infectivity of Toxoplasma gondii and Cryptosporidium parvum oocysts bioaccumulated by Dreissena polymorpha

et al. 2020

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Aims The study was aimed to understand the depuration process of Cryptosporidium parvum and Toxoplasma gondii oocysts by zebra mussel (Dreissena polymorpha), to consider the use of the zebra mussel as a bioremediation tool. Materials and methods Two experiments were performed: (i) individual exposure of mussel to investigate oocyst transfers between bivalves and water and (ii) in vivo exposure to assess the ability of the zebra mussel to degrade oocysts. Results (i) Our results highlighted a transfer of oocysts from the mussels to the water after 3 and 7 days of depuration; however, some oocysts were still bioaccumulated in mussel tissue. (ii) Between 7 days of exposure at 1000 or 10 000 oocysts/mussel/day and 7 days of depuration, the number of bioaccumulated oocysts did not vary but the number of infectious oocysts decreased. Conclusion Results show that D. polymorpha can release oocysts in water via (pseudo)faeces in depuration period. Oocysts remain bioaccumulated and infectious oocyst number decreases during the depuration period in zebra mussel tissues. Results suggest a degradation of bioaccumulated C. parvum and T. gondii oocysts. Significance and Impact of the Study This study highlighted the potential use of D. polymorpha as a bioremediation tool to mitigate of protozoan contamination in water resources.

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First evidence of cytotoxic effects of human protozoan parasites on zebra mussel (Dreissena polymorpha) haemocytes

Cited by 6 publications

References 32 publications

Cellular and molecular complementary immune stress markers for the model species Dreissena polymorpha

Cellular and molecular complementary immune stress markers for the model species Dreissena polymorpha

Blue mussel ( Mytilus edulis )—A bioindicator of marine water contamination by protozoa: Laboratory and in situ approaches

Survival and infectivity of Toxoplasma gondii and Cryptosporidium parvum oocysts bioaccumulated by Dreissena polymorpha

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