First Evidence of Porcine Circovirus Type 2 (PCV‐2) Infection of Pigs in the Czech Republic by Semi‐Nested PCR

Celer, V.; Carasová, P.

doi:10.1046/j.1439-0450.2002.00547.x

Cited by 11 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Porcine circovirus type 2 (PCV2) as an etiological agent of porcine circovirus diseases (PCVDs) was firstly identified in the 1990s in Canada [ 1 ], and was later detected and characterized in USA and Europe [ 2 , 3 ]. In the Czech Republic and Slovakia PCV2 in pigs was confirmed and genetically typed a few years later [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

The presence of six potentially pathogenic viruses in pigs suffering from post-weaning multisystemic wasting syndrome

et al. 2014

View full text Add to dashboard Cite

BackgroundPorcine circovirus type 2 (PCV2) is an etiological agent of porcine circovirus diseases (PCVDs). Post-weaning multisystemic wasting syndrome (PMWS) as the most important PCVD is considered a multifactorial disease. It was demonstrated that not only PCV2 but several viruses are associated with PMWS. Studies of viral co-infections in PMWS pigs led often to controversial results. The aim of this work was to determine the presence of emerging (PRRSV), re-emerging (PTV) and newly-emerging (TTSuV1, TTSuV2, PBoV1) viruses in samples of dead pigs suffering from PMWS. The impact of vaccination against PCV2 and the influence of age on the occurrence of single and multiple viral infections in pigs were also investigated.ResultsViruses were detected by PCR, RT-PCR and real-time PCR in the pooled tissue samples (lymph nodes, liver and spleen) of pigs with PMWS (n = 56) which were divided into three groups: suckling piglets, post-weaning pigs and fattening pigs. In addition, lymph node samples were collected from apparently healthy fattening pigs (n = 59). The effect of vaccination against PCV2 with Ingelvac CircoFlex vaccine was also investigated. Between non-vaccinated pigs, the highest prevalence of individual viruses and multiple viral infections were found in diseased post-weaning and fattening animals with PMWS. Severe clinical disease was observed in swine co-infected with PCV2 and PRRSV. The prevalence of TTSuV1 and TTSuV2 was high in all groups of pigs and did not appear to have a significant effect on the syndrome. Simultaneous infection with TTSuV1 and PBoV1 was frequently confirmed in pigs with PMWS. No healthy pig was found to be infected with PRRSV, PTV or PBoV1. Vaccination against PCV2 did not influence the prevalence of TTSuVs, but significantly protected pigs against multiple viral infections.ConclusionsPost-weaning PMWS pigs were more often co-infected with viral pathogens than suckling or fattening pigs. Co-infection with PRRSV enforces clinical signs of PMWS, the influence of other viral co-infections is not clear. Vaccination against PCV2 significantly reduced viral co-infections in pigs.

show abstract

Section: Introductionmentioning

confidence: 99%

The presence of six potentially pathogenic viruses in pigs suffering from post-weaning multisystemic wasting syndrome

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Although it was shown that this virus can be transmitted vertically Ladekjaer-Mikkelson et al, 2001;O'Connor et al, 2001;Sanchez et al, 2001] there is a common view that this virus is associated with severe pathology in newborn piglets, and its vertical transmission is a rare event. Three facts call special attention in assessment of the infection risk associated with xenotransplantation: ubiquity of the PCV type 2 virus [Labarque et al, 2000;Trujano et al, 2001;Celera and Carasova, 2002;Kim et al, 2002;Liu, 2002;Wattrang et al, 2002;Garka- [Hattermann, 2002, own unpublished data], and in experimental mice in vivo [Kiupel, 2001]. All these facts need more intensive study.…”

Section: Discussionmentioning

confidence: 99%

Monitoring for potentially xenozoonotic viruses in New Zealand pigs

Garkavenko¹,

Muzina²,

Muzina³

et al. 2003

Journal of Medical Virology

View full text Add to dashboard Cite

Shortage of human donor organs for transplantation has prompted evaluation of animals as an alternative donor source. Pigs are the most acceptable candidate animals but issues of xenozoonozes remain. Despite careful monitoring of high-health-status (HHS) pigs, there is still a risk that their tissues may carry infectious agents. Furthermore, pathogens which are significant in xenotransplantation are not necessarily those of veterinary importance. The detection of these potentially transmissible infectious agents may require the development and application of new surveillance technologies. We present data on monitoring for five potentially xenotic viruses in New Zealand pig herds, namely pig cytomegalovirus (PCMV), pig lymphotropic herpesvirus (PLHV), encephalomyocarditis virus (EMCV), pigcircovirus (PCV), and hepatitis E virus (HEV). These five viruses are either potentially oncogenic, establish persistent infection, or are known to be zoonotic. This study has expanded significantly the information on porcine viruses in New Zealand. Using this information, it is now possible to complete protocols for monitoring pig herds and tissues prior to their use in xenotransplantation. The study resulted in selection of a possible source herd for swine-to-human islet transplantation.

show abstract

“…Three factors must be considered when assessing the infectious risk of PCV2 associated with xenotransplantation: (1) the ubiquity of the PCV type 2 virus (Celera & Carasova, 2002;Garkavenko et al, 2005;Kim et al, 2002;Labarque, Nauwynck, Mesu, & Pesaert, 2000;Trujano, Iglesias, Segales, & Palacios, 2001;Wattrang et al, 2002); (2) the potential that the virus might be transmitted vertically without showing any sign of abnormality and (3) transmission of the virus occurs in human cells in vitro (Hattermann, 2002, unpublished data), and in experimental mice in vivo (Kiupel et al, 2001). Despite evidence that caesarean section and barrier rearing techniques are effective in excluding PCV and PLHV from pig populations, these interventions do not guarantee the exclusion of all viruses from a pig herd (Tucker et al, 2003).…”

Section: Determining Infections Relevant To Xenotransplantation In Nzmentioning

confidence: 99%

Developing Xenostandards for Microbiological Safety: New Zealand Experience

Garkavenko¹,

Wynyard²,

Nathu³

et al. 2012

Xenotransplantation

View full text Add to dashboard Cite

First Evidence of Porcine Circovirus Type 2 (PCV‐2) Infection of Pigs in the Czech Republic by Semi‐Nested PCR

Cited by 11 publications

References 17 publications

The presence of six potentially pathogenic viruses in pigs suffering from post-weaning multisystemic wasting syndrome

The presence of six potentially pathogenic viruses in pigs suffering from post-weaning multisystemic wasting syndrome

Monitoring for potentially xenozoonotic viruses in New Zealand pigs

Developing Xenostandards for Microbiological Safety: New Zealand Experience

Contact Info

Product

Resources

About