First evidence of somatic embryogenesis from needles of 1-year-old Picea abies plants

Ruaud, Jean-Noël; Bercetche, J.; Pâques, M.

doi:10.1007/bf00233093

Cited by 35 publications

(17 citation statements)

References 15 publications

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“…Plants produced by means of somatic embryogenesis are very good donors of explants, because their tissues are physiologically younger than zygotic embryos. The potential for embryogenic tissue initiation by explants decreases with age (Ruaud 1993;Ruaud et al 1992;Harvength et al 2001). Ruaud et al (1992) used as sources of explants some needles of one-year-old somatic and zygotic seedlings of P. abies.…”

Section: Induction Of Embryogenic Tissuementioning

confidence: 99%

“…The potential for embryogenic tissue initiation by explants decreases with age (Ruaud 1993;Ruaud et al 1992;Harvength et al 2001). Ruaud et al (1992) used as sources of explants some needles of one-year-old somatic and zygotic seedlings of P. abies. A higher frequency of embryogenic tissue initiation was achieved by those authors from the former type of explants (80%), while only 10% of needles of zygotic seedlings were able to regenerate embryogenic tissue.…”

Section: Induction Of Embryogenic Tissuementioning

confidence: 99%

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Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

Hazubska-Przybył

Bojarczuk

2011

Acta Soc Bot Pol

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Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens Glauca and P. breweriana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. breweriana) to 23.75% (P. omorika and P. pungens Glauca). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g·l -1 ). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No embryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.

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Section: Induction Of Embryogenic Tissuementioning

confidence: 99%

Section: Induction Of Embryogenic Tissuementioning

confidence: 99%

Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

Hazubska-Przybył

Bojarczuk

2011

Acta Soc Bot Pol

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“…For initiation of embryogenic tissue in spruce, researchers have used immature (Hakman et al 1985) and mature zygotic embryos (Gupta and Durzan 1986;von Arnold 1987;Adams et al 1994;Hazubska and Szczygie³ 2003) or cotyledons and hypocotyls of germinating zygotic or somatic embryos and needles (Lelu et al 1987;Lelu and Bornman 1990;Ruaud et al 1992;Ruaud 1993). Recently, Harvengt et al (2001) indicated that embryogenic callus can be initiated from needles of 3-year-old somatic seedlings.…”

Section: Embryogenic Callus Initiationmentioning

confidence: 99%

Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix

2011

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Experiments on somatic embryogenesis in selected spruce, fir and larch species were performed to determine if this method of micropropagation enables production of quality seedlings for forest nurseries. High frequencies of embryogenic callus in spruce (23-31%) and fir (29%) were achieved when mature zygotic embryos were used as explants, while in larches (36%) only when megagametophytes (endosperms with immature embryos) were used. The possibility of somatic embryogenesis initiation also from somatic embryos (resulting in secondary and third generation of embryogenic callus) indicate high efficiency of this method of micropropagation. The best results at all stages of somatic embryogenesis (good proliferation of embryogenic callus, high rate of embryo regeneration and their survival) were obtained for Norway spruce, European larch and some hybrid larches.

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“…También se puede lograr embriogénesis a partir de elementos florales (Merkle y Battle, 2000;Toribio et al, 2000), pero en este caso hay que confirmar si se trata de embriogénesis somática o gamética. En algunas especies de coníferas se ha logrado la inducción de embriogénesis somática a partir de tejidos no embrionarios (Atree et al, 1990;Ruaud et al, 1992), pero las referencias sobre árboles adultos son todavía escasas, como la patente sobre Pinus pinaster, citada en Ramarosandratana et al (1999), y la inducción en ápices vegetativos de ár-boles de 20 años en Pinus radiata (Smith, 1999) y de 15 años en Pinus patula (Malabadi y van Staden, 2005).…”

Section: Las Fases De La Regeneración Por Embriogénesis Somática Induunclassified

Somatic embryogenesis as a main tool in forest biotechnology

Celestino¹,

Hernández²,

Carneros³

et al. 2005

For. syst.

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ResumenPara dar sentido agronómico a diferentes biotecnologías de aplicación, esto es, para poder transferir las posibilidades que ofrecen a la actividad operativa, es necesario disponer de una técnica fiable de regeneración de plantas. El cultivo de tejidos de genotipos valiosos, de meristemos libres de virus, de células transformadas, etc., no sirve de nada desde un punto de vista aplicado si de ellos no es posible conseguir la multiplicación en masa de dichos genotipos selectos, obtener plantas saneadas o lograr plantas transgénicas, en condiciones económicamente viables. La embriogénesis somática está ampliamente considerada como la mejor vía de regeneración que utiliza las técnicas de cultivo de tejidos vegetales en biotecnología forestal. Los avances actuales en biotecnología-genómica están teniendo un gran impacto en el uso de material selecto en muchos programas de mejora. La embriogénesis somática está contribuyendo a la producción de plantas transgénicas y a la consecución de la silvicultura clonal de alta productividad, con calidad de madera mejorada y menor impacto de enfermedades, en plantaciones forestales. Este artículo revisa la situación actual de la embriogénesis somática aplicada a especies forestales.Palabras clave: Regeneración de plantas, cultivo in vitro de tejidos vegetales. Summary Somatic embryogenesis as a main tool in forest biotechnologyTo give agronomic value, that is, to be able to transfer the benefits of different biotechnologies of application to operational purposes, a reliable plant regeneration technique is needed. Tissue culture of valuable genotypes, of virus-free meristems, of transformed cells, etc., is not worth if we are not able to mass propagating clonal plants, to obtain healthy plants or to achieve transgenic plants. Somatic embryogenesis is widely assumed to be the best tissue culture-based way of regenerating plants in forest biotechnology. Recent advances in biotechnology-genomics are having an important effect on the deployment of selected genetic plant materials in many breeding programs. Somatic embryogenesis is contributing to the release of transformed plants and to the implementation of clonal forestry with improved productivity, enhanced wood quality and reduced diseases in forest plantations. This article review the current status of somatic embryogenesis applied to forest species.

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First evidence of somatic embryogenesis from needles of 1-year-old Picea abies plants

Cited by 35 publications

References 15 publications

Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix

Somatic embryogenesis as a main tool in forest biotechnology

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