First Evidence of Vertical Infection of Dengue Virus 2 in<i>Aedes aegypti</i>Mosquitoes from Sinaloa, Mexico

Apodaca-Medina, Annete I.; Torres-Avendaño, José Israel; Rendón-Maldonado, José Guadalupe; Torres-Montoya, Edith Hilario; Flores-López, Beatriz Armida; Ángel, Rosa M. del; Velarde‐Félix, Jesús Salvador; Salomón-Soto, Víctor Manuel; Castillo-Ureta, Hipólito

doi:10.1089/vbz.2017.2202

Cited by 10 publications

(3 citation statements)

References 8 publications

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“…Under laboratory conditions, the filial infectious rate of Zika virus in Ae. aegypti mosquitoes ranged from 4.9 to 24.6, depending on the strain of the virus tested ( 31 ), which corroborates that our results are comparable to the MIRs reported for other Zika viruses and other flaviviruses, including DENV ( 32 , 33 ).…”

Section: Discussionsupporting

confidence: 91%

Natural Vertical Transmission of Zika Virus in Larval Aedes aegypti Populations, Morelos, Mexico

Izquierdo-Suzán¹,

Zárate²,

Torres-Flores³

et al. 2019

Emerg. Infect. Dis.

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We characterized natural vertical transmission of Zika virus in pools of Aedes aegypti larvae hatched from eggs collected in Jojutla, Morelos, Mexico. Of the 151 pools analyzed, 17 tested positive for Zika virus RNA; infectious Zika virus was successfully isolated from 1 of the larvae pools (31N) in C6/36 cells. Real-time quantitative PCR and indirect immunofluorescence assays confirmed the identity of the isolate, named Zika virus isolate 31N; plaque assays in Vero cells demonstrated the isolate’s infectivity in a mammalian cell line. We obtained the complete genome of Zika virus isolate 31N by next-generation sequencing and identified 3 single-nucleotide variants specific to Zika virus isolate 31N using the meta-CATS tool. These results demonstrate the occurrence of natural vertical transmission of Zika virus in wild Ae. aegypti mosquitoes and suggest that this transmission mode could aid in the spread and maintenance of Zika virus in nature.

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Section: Discussionsupporting

confidence: 91%

Natural Vertical Transmission of Zika Virus in Larval Aedes aegypti Populations, Morelos, Mexico

Izquierdo-Suzán¹,

Zárate²,

Torres-Flores³

et al. 2019

Emerg. Infect. Dis.

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“…Additionally, DENV-2 was identified in three Ae. aegypti larvae pools in Quintana Roo State and two pools in Sinaloa State [16,18]. However, in none of these studies, virus infection was confirmed by virus isolation.…”

Section: Discussionmentioning

confidence: 90%

Vertical transmission of dengue virus in Aedes aegypti and its role in the epidemiological persistence of dengue in Central and Southern Mexico

Danis‐Lozano

Díaz-González

Malo-García

et al. 2019

Tropical Med Int Health

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Background Aedes aegypti and Aedes albopictus are the main mosquito species responsible for dengue virus (DENV) transmission to humans in the tropical and subtropical regions of the world. The role of vertical transmission in the epidemiology of dengue and the maintenance of this arbovirus in nature during interepidemic periods remain poorly understood, and DENV vertical transmission could sustain the existence of virus reservoirs within Aedes populations. Methods Between April 2011 and October 2012, we monitored vertical transmission of DENV in Ae. aegypti and Ae. albopictus in 9 cities of 4 Mexican states. Aedes eggs were collected in ovitraps, then adults were reared under laboratory conditions and their heads were used to infect C6/36 cells. The presence of flavivirus was detected by immunofluorescence assays (IFA), and DENV infection was confirmed by RT‐PCR. Results About 96% of reared adults were Ae. aegypti and 4.0% were Ae. albopictus. No infection was detected in Ae. albopictus, whereas 54 of 713 (7.8%) of Ae. aegypti pools tested positive. A minimum infection rate (MIR) of 2.52 per 1000 mosquitoes was estimated for Ae. aegypti. DENV‐1, DENV‐2 & DENV‐3 serotypes were detected even during interepidemic periods. Conclusions This study reports the evidence of vertical transmission of dengue virus with viral isolation and molecular confirmation in Ae. aegypti eggs collected in four endemic regions of Central and Southern Mexico. Vertical transmission may play a role as a reservoir mechanism during mosquito dormancy in interepidemic periods but with minor participation in transmission during epidemic periods.

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“…Similarly, in Sinaloa, Mexico, RT-PCR and semi-nested PCR were used to identify DENV-2 in 2 of 14 pools of Ae. aegypti larvae (308 larvae) [ 41 ] and DENV-4 in 15 of 36 pools of Ae. aegypti larvae (672) [ 42 ] collected from the field with MIRs of 6.49 and 22.32, respectively.…”

Section: Introductionmentioning

confidence: 99%

Natural vertical transmission of dengue virus in Latin America and the Caribbean: highlighting its detection limitations and potential significance

Golding,

Noble,

Khouri

et al. 2023

Parasites Vectors

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Dengue continues to be a major public health concern in Latin America and the Caribbean with many countries in the region having experienced drastic increases in the incidence of dengue over the past few years. Dengue virus is predominantly transmitted by the bite of an infected female Aedes aegypti mosquito via a process called horizontal transmission. However, the virus may also be transmitted from an infected female mosquito to her offspring by vertical transmission, which occurs via viral invasion of the ovary either at the time of fertilization or during oviposition. In this way, mosquitoes may become dengue virus infected before ever encountering a human host. While some researchers have reported this phenomenon and suggested it may serve as a reservoir for the dengue virus in nature, others have questioned its epidemiological significance because of the low frequency at which it has been observed. Several researchers have either altogether failed to detect it or observed its occurrence at low frequencies. However, some studies have attributed these failures to small sample sizes as well as poor sensitivities of screening methods employed. Therefore, an overview of the occurrence, significance and limitations of detection of vertical transmission of dengue virus in Aedes mosquitoes in nature within Latin America and the Caribbean will be the focus of this review. Graphical Abstract

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First Evidence of Vertical Infection of Dengue Virus 2 inAedes aegyptiMosquitoes from Sinaloa, Mexico

Cited by 10 publications

References 8 publications

Natural Vertical Transmission of Zika Virus in Larval Aedes aegypti Populations, Morelos, Mexico

Natural Vertical Transmission of Zika Virus in Larval Aedes aegypti Populations, Morelos, Mexico

Vertical transmission of dengue virus in Aedes aegypti and its role in the epidemiological persistence of dengue in Central and Southern Mexico

Natural vertical transmission of dengue virus in Latin America and the Caribbean: highlighting its detection limitations and potential significance

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