First Experimental Evidence for Reversibility of Ammonia Loss from Asparagine

Wang, Jijing; Rodin, Sergey; Saei, Amir Ata; Zhang, Xuepei; Zubarev, Roman A.

doi:10.3390/ijms23158371

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…There is a negative relationship between the asparagine content and protein lifetime. However, some studies have suggested that spontaneous deamidation of asparagine generates an isoaspartate residue that hampers protein function and induces disorders associated with senescence [ 10 ]. Sequence- and structure-based methods can detect asparagine deamidation in proteins [ 11 ]; thus, we can predict the function of proteins through these methods.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of asparagine in the physiological state and cancer

Yuan,

Yin,

et al. 2024

Cell Commun Signal

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Asparagine, an important amino acid in mammals, is produced in several organs and is widely used for the production of other nutrients such as glucose, proteins, lipids, and nucleotides. Asparagine has also been reported to play a vital role in the development of cancer cells. Although several types of cancer cells can synthesise asparagine alone, their synthesis levels are insufficient to meet their requirements. These cells must rely on the supply of exogenous asparagine, which is why asparagine is considered a semi-essential amino acid. Therefore, nutritional inhibition by targeting asparagine is often considered as an anti-cancer strategy and has shown success in the treatment of leukaemia. However, asparagine limitation alone does not achieve an ideal therapeutic effect because of stress responses that upregulate asparagine synthase (ASNS) to meet the requirements for asparagine in cancer cells. Various cancer cells initiate different reprogramming processes in response to the deficiency of asparagine. Therefore, it is necessary to comprehensively understand the asparagine metabolism in cancers. This review primarily discusses the physiological role of asparagine and the current progress in the field of cancer research.

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Section: Introductionmentioning

confidence: 99%

Metabolism of asparagine in the physiological state and cancer

Yuan,

Yin,

et al. 2024

Cell Commun Signal

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“…Besides reviewing the available theoretical , we reported the first experimental evidence on the reversibility of isoAsp via protein succinimide/ isoaspartate ammonia ligase (PSIAL) activity[7]. Such activity was found in e.g.…”

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confidence: 99%

“…Being intrigued by the apparently irreversible nature of deamidation that seems to be in stark contrast with observed cell self-renewal phenomena (e.g., totally normal “young” babies born by woman 60 and more years old), we investigated the possibility of true isoAsp-to-Asn repair. Besides reviewing the available theoretical arguments, we reported the first experimental evidence on the reversibility of isoAsp via protein succinimide/isoaspartate ammonia ligase (PSIAL) activity [ 7 ]. Such activity was found in e.g.…”

mentioning

confidence: 99%