First finding of Echinococcus multilocularis DNA in soil: Preliminary survey in Varmia-Masuria Province, northeast Poland

Szostakowska, Beata; Lass, Anna; Kostyra, K.; Pietkiewicz, Halina; Myjak, P

doi:10.1016/j.vetpar.2014.02.028

Cited by 26 publications

(34 citation statements)

References 24 publications

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“…As has been pointed out by Umhang et al (2017), there is a need to improve the standardization of technical options to study samples such as soil, water or sediments. Very few data are currently available on Echinococcus species in soil and other environmental matrices (Shaikenov et al, 2004;Szostakowska et al, 2014;Lass et al, 2015;Umhang et al, 2017). One technical option proposed to confirm them as E. granulosus eggs is polymerase chain reaction (PCR).…”

Section: Discussionmentioning

confidence: 99%

Dispersion of Echinococcus granulosus eggs from infected dogs under natural conditions in Patagonia, Argentina

et al. 2019

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Cystic echinococcosis caused by Echinococcus granulosus is a major zoonosis of public health significance in the Patagonian region of Argentina. This investigation sought to test the hypothesis that the persistence and dispersion of the parasite eggs can be explained by physical and meteorological parameters along with final host infection and behaviour. This observational study was carried out over a five-year period within an enclosure where two dogs harbouring a worm burden ranging from 100 to 1000 mature adult E. granulosus, as well as two uninfected dogs, had previously been kept for six months. Environmental canine faeces, topsoil, pond water, and sediment samples were examined to control for the presence of eggs and coproantigens of the parasite using microscope-based techniques and copro-ELISA plus copro-Western Blot tests. The parasite eggs were detected up to 41 months later in faeces from infected dogs, soil and sediment, and coproantigen tests remained positive for up to 70 months in faeces. Overall, parasite eggs were found within a maximum distance of 115 m from the contaminated dog faeces deposition site. Our findings indicate that under Patagonian environmental conditions, egg persistence and dispersion seem to be related to the worm burden and habits of the infected dog, to prevailing wind direction and to the existence of low bushes as well as natural bodies of water. The present study is the first to provide direct evidence of interaction between bioclimatic conditions and E. granulosus egg dispersion under Patagonian field conditions. https://www.cambridge.org/core/terms. https://doi.

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Section: Discussionmentioning

confidence: 99%

Dispersion of Echinococcus granulosus eggs from infected dogs under natural conditions in Patagonia, Argentina

et al. 2019

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“…It again shows that the ru-ral environment close to human houses in an endemic area of Poland may be contaminated by the eggs of E. multilocularis. The parasite had been previously detected directly in soil (Szostakowska et al 2014) and indirectly using pigs as an indicator (Karamon et al 2012). Despite the low prevalence of E. multilocularis in rural dogs, they may be an important source of infection for humans due to their close contact.…”

Section: Discussionmentioning

confidence: 99%

First detection of Echinococcus multilocularis in dogs in a highly endemic area of Poland

Karamon¹,

Samorek-Pieróg²,

Kochanowski³

et al. 2016

FOLIA PARASIT

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Abstract:The aim of the investigation was to estimate the epizootic situation concerning infection by the cestode Echinococcus multilocularis Leuckart, 1863 in dogs (Canis lupus familiaris Linnaeus) from a Polish region where this parasite is highly prevalent in red foxes. Faecal samples (n = 148) were collected from rural dogs in Podkarpackie Province. Samples were examined through nested PCR (for E. multilocularis), multiplex PCR (E. multilocularis, species of Taenia Linnaeus, 1758) and PCR [E. granulosus (Batsch, 1786)]. Specific products were sequenced. Faeces were also examined coproscopically. In samples from two dogs (1.4%), there were positive PCR results for E. multilocularis. Taenia-specific PCR products were found in nine dogs (6.1%). Sequencing identified Taenia serialis (Gervais, 1847), T. hydatigena Pallas, 1766, T. pisiformis (Bloch, 1780) and Hydatigera taeniaeformis (Batsch, 1786). One sample (0.7%) was identified as Mesocestoides litteratus (Batsch, 1786). All samples were negative for E. granulosus with PCR. Taking into account coproscopic and PCR results, 28% of dogs were infected with helminths (8% with tapeworms). It should be stressed that one of the infected with E. multilocularis dogs shed eggs of the Taenia type and had a habit of preying on rodents. This investigation revealed the presence of E. multilocularis in dogs for the first time in Poland.

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“…While most researchers who use genetic methods to detect sth in environmental matrices perform nucleic acid extraction after recovering ova by flotation [66, 84-86], some have successfully performed extraction directly on the environmental matrix [87, 88]. Some protocols call for disrupting the outer shell of certain helminth ova (primarily trichuris and baylisascaris procyonis ) in order to efficiently extract genetic material; this can be accomplished through freeze-thaw cycles [85, 86], heating/boiling [85], shaking with glass beads [84, 85], or digestion with proteinase k [84, 86].…”

Section: Quantification Of Sth Larvae and Ovamentioning

confidence: 99%

Methods for Quantification of Soil-Transmitted Helminths in Environmental Media: Current Techniques and Recent Advances

Collender

Kirby

Addiss

et al. 2015

Trends in Parasitology

100

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Limiting the environmental transmission of soil-transmitted helminths (STH), which infect 1.5 billion people worldwide, will require sensitive, reliable, and cost effective methods to detect and quantify STH in the environment. We review the state of the art of STH quantification in soil, biosolids, water, produce, and vegetation with respect to four major methodological issues: environmental sampling; recovery of STH from environmental matrices; quantification of recovered STH; and viability assessment of STH ova. We conclude that methods for sampling and recovering STH require substantial advances to provide reliable measurements for STH control. Recent innovations in the use of automated image identification and developments in molecular genetic assays offer considerable promise for improving quantification and viability assessment.

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First finding of Echinococcus multilocularis DNA in soil: Preliminary survey in Varmia-Masuria Province, northeast Poland

Cited by 26 publications

References 24 publications

Dispersion of Echinococcus granulosus eggs from infected dogs under natural conditions in Patagonia, Argentina

Dispersion of Echinococcus granulosus eggs from infected dogs under natural conditions in Patagonia, Argentina

First detection of Echinococcus multilocularis in dogs in a highly endemic area of Poland

Methods for Quantification of Soil-Transmitted Helminths in Environmental Media: Current Techniques and Recent Advances

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