First genotyping of<i>Giardia duodenalis</i>and prevalence of enteroparasites in children from Tetouan (Morocco)

Fatni, Chadia El; Olmo, F.J.; Fatni, H El; Romero, Desirée; Rosales, María J.

doi:10.1051/parasite/2014049

Cited by 39 publications

(31 citation statements)

References 22 publications

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“…However, assemblage B seems to be more human specific [ 43 ]. Our results are consistent with other studies among children in other countries such as Brazil, Nepal, and Iran reporting a predominance of assemblage B [ 82 ]. Additionally, the association between assemblage occurrence and the age of patients showing higher risk of assemblage B infection in children under 12 years old has been described [ 83 ].…”

Section: Discussionsupporting

confidence: 93%

Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon

Osman¹,

Safadi²,

Cian³

et al. 2016

PLoS Negl Trop Dis

144

115

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BackgroundIntestinal protozoan infections are confirmed as major causes of diarrhea, particularly in children, and represent a significant, but often neglected, threat to public health. No recent data were available in Lebanon concerning the molecular epidemiology of protozoan infections in children, a vulnerable population at high risk of infection.Methodology and Principal FindingsIn order to improve our understanding of the epidemiology of intestinal pathogenic protozoa, a cross-sectional study was conducted in a general pediatric population including both symptomatic and asymptomatic subjects. After obtaining informed consent from the parents or legal guardians, stool samples were collected in January 2013 from 249 children in 2 schools in Tripoli, Lebanon. Information obtained from a standard questionnaire included demographic characteristics, current symptoms, socioeconomic status, source of drinking water, and personal hygiene habits. After fecal examination by both microscopy and molecular tools, the overall prevalence of parasitic infections was recorded as 85%. Blastocystis spp. presented the highest infection rate (63%), followed by Dientamoeba fragilis (60.6%), Giardia duodenalis (28.5%) and Cryptosporidium spp. (10.4%). PCR was also performed to identify species and genotypes of Cryptosporidium, subtypes of Blastocystis, and assemblages of Giardia. Statistical analysis using a logistic regression model showed that contact with family members presenting gastrointestinal disorders was the primary risk factor for transmission of these protozoa.ConclusionsThis is the first study performed in Lebanon reporting the prevalence and the clinical and molecular epidemiological data associated with intestinal protozoan infections among schoolchildren in Tripoli. A high prevalence of protozoan parasites was found, with Blastocystis spp. being the most predominant protozoans. Although only 50% of children reported digestive symptoms, asymptomatic infection was observed, and these children may act as unidentified carriers. This survey provides necessary information for designing prevention and control strategies to reduce the burden of these protozoan infections, especially in children.

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Section: Discussionsupporting

confidence: 93%

Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon

Osman¹,

Safadi²,

Cian³

et al. 2016

PLoS Negl Trop Dis

144

115

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“…The high prevalence we found is similar to those reported by other recent studies in developing countries. In Morocco, Blastocystis hominis (64%) was the most prevalent parasite in four schools located in both urban and rural areas of Tetouan 23 , and in Colombia the prevalence was 57.5% 5 .…”

Section: Discussionmentioning

confidence: 99%

HIGH PREVALENCE OF Blastocystis spp. INFECTION IN CHILDREN AND STAFF MEMBERS ATTENDING PUBLIC URBAN SCHOOLS IN SÃO PAULO STATE, BRAZIL

Rebolla

Silva

Gomes

et al. 2016

Rev. Inst. Med. trop. S. Paulo

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SUMMARYAfter a gastroenteritis outbreak of unknown etiology in the municipality of Sebastião da Grama, São Paulo, Brazil, we conducted a parasitological survey to establish the epidemiological profile of enteroparasitosis in children and staff members attending the public urban schools in operation in town. The cross-sectional study evaluated 172 children aged 11 months to 6 years old and 33 staff members aged 19 to 58 years old. Overall, 96 (55.81%) children and 20 (60.61%) staff members were mono-parasitized, while 58 (33.72%) children and 4 (12.12%) workers were poly-parasitized. Protozoa (88.37%; 72.73%) was more prevalent than helminthes (3.48%; 0%) in children and staff members respectively. Blastocystis spp. was the most prevalent parasite in children (86.63%) and staff members (66.67%). The age of 1 year old or less was found to be associated with increased prevalence of giardiasis [OR = 13.04; 95%CI 2.89-58.91; p = 0.00] and public garbage collection was identified as a protective factor against intestinal helminth infections [OR = 0.06; 95%CI 0.00-0.79; p = 0.03]. Although most of the children tested positive for Blastocystis spp. and also presented clinical signs/symptoms (62.2%), this association was not statistically significant [OR = 1.35; 95%CI 0.53-3.44; p = 0.51]. Intestinal parasites still represent a public health concern and this study underscores the importance of further investigations to better understand the pathogenic role of Blastocystis spp.

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“…[14][15][16] Another study reported a prevalence of 39.4% by DLM in a population of 673 schoolchildren in the area of Tetouan, Morocco. 17 In Libya, the overall prevalence of Blastocystis sp. infection ranged from 22.1% to 28% by xenic in vitro culture followed by DLM in three different cohorts of patients attending the Central Laboratory in Sebha.…”

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confidence: 99%

Prevalence and Subtype Identification of Blastocystis sp. in Healthy Individuals in the Tunis Area, Tunisia

Abda¹,

Maatoug²,

Romdhane³

et al. 2017

The American Society of Tropical Medicine and Hygiene

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Abstract. Blastocystis sp. is currently the most common eukaryotic parasite found in humans. Despite its potential public health impact, epidemiological data regarding its prevalence and molecular subtype (ST) distribution in Maghreb are rarely reported. Therefore, the aim of this study was to determine the prevalence of the parasite in a cohort of healthy food handler Tunisian individuals and to acquire the first molecular data regarding the distribution of Blastocystis sp. STs in this country. Therefore, 524 fecal samples were collected, and 68 of them (13%) were identified as positive for the parasite by direct-light microscopy of smears. Seventeen samples of 100 negative by microscopy were also shown to be positive by real-time quantitative polymerase chain reaction. Among all the positive samples, 61 Blastocystis isolates were subtyped using partial small subunit ribosomal RNA gene analysis. ST3 was the most abundant (51%) followed by ST1 (30%), ST2 (16%), and ST4 and ST7 (both 1.6%).Blastocystis sp. is a parasite inhabiting the gastrointestinal tract of humans and a wide range of animals. This singlecelled eukaryote is currently the most prevalent protozoa found in human fecal samples with a worldwide distribution.

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First genotyping ofGiardia duodenalisand prevalence of enteroparasites in children from Tetouan (Morocco)

Cited by 39 publications

References 22 publications

Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon

Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon

HIGH PREVALENCE OF Blastocystis spp. INFECTION IN CHILDREN AND STAFF MEMBERS ATTENDING PUBLIC URBAN SCHOOLS IN SÃO PAULO STATE, BRAZIL

Prevalence and Subtype Identification of Blastocystis sp. in Healthy Individuals in the Tunis Area, Tunisia

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