First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring
            <i>bla</i>
            <sub>CTX-M-14</sub>
            in South America

Bado, Inés; García-Fulgueiras, Virginia; Cordeiro, Nicolás F.; Betancor, Laura; Caiata, Leticia; Seija, Verónica; Robino, Luciana; Algorta, Gabriela; Chabalgoity, José A.; Ayala, Juan A.; Gutkind, Gabriel; Vignoli, Rafael

doi:10.1128/aac.05530-11

Cited by 23 publications

(16 citation statements)

References 28 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this regard, we recently reported the occurrence of bla CTX-M-14 in an isolate of Salmonella enterica serovar Enteritidis, encoded in a similar plasmid [40]. Additionally, in our continent, IncI1 plasmids harboring bla CTX-M-14 and bla CTX-M-19 (both belonging to group 4 in the CTX-M family) have already been described in E. coli strains in our country and in Bolivia [14,36].…”

Section: Discussionsupporting

confidence: 57%

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Vignoli

García-Fulgueiras

Cordeiro

et al. 2016

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored bla CTX-M-15 (67.3%), eight bla CTX-M-2 (14.6%), five bla CTX-M-14 (9.1%), three carried both bla CTX-M-2 and bla CTX-M-14 , one bla CTX-M-9 , and one bla CTX-M-8 . Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6')Ib-cr as well. Isolates harboring bla CTX-M-2 , bla CTX-M-14 , bla CTX-M-9 , or bla CTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.

show abstract

Section: Discussionsupporting

confidence: 57%

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Vignoli

García-Fulgueiras

Cordeiro

et al. 2016

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

show abstract

“…Jin and Ling 36 reported the presence of bla-CTX-M genes on 62 kb, 70 kb, and 92 kb harbored by S. enteritidis and S. typhimurium from Hong Kong, while Mshana et al 31 found a 231 kb plasmid to be responsible for the carriage of bla-CTX-M-15 gene among S. enterica isolates from Tanzania. In a study carried out by Bado et al 37 in Uruguay, where the first description of bla CTX-M-I gene cluster in S. enteritidis was reported, a 195 kb plasmid was incriminated as a vector. In Germany, Fischer et al 38 reported the transferability of blaCTX-M-15 among Salmonella isolates from horse and swine through 95 kb IncF and Inc1I plasmids.…”

Section: Discussionmentioning

confidence: 99%

blaCTX-M-I group extended spectrum beta lactamase-producing Salmonella typhi from hospitalized patients in Lagos, Nigeria

Akinyemi¹,

Iwalokun²,

Alafe³

et al. 2015

IDR

View full text Add to dashboard Cite

PurposeThe global spread of blaCTX-M-I extended-spectrum beta-lactamase (ESBL)-producing Salmonella spp. remains a major threat to treatment and control. Evidence of emergence and spread of this marker are lacking in Nigeria. This study investigated blaCTX-M-I ESBL production among Salmonella isolates from hospitalized patients.MethodsPatients (158 total) made up of two groups were evaluated. Group A was composed of 135 patients with persistent pyrexia and group B was composed of 23 gastroenteritis patients and their stool samples. Samples were cultured, and isolates were identified and were subjected to antibiotic susceptibility testing by standard methods. Isolates were further screened for ESBL production, blaCTX-M-I genes and transferability by double disk synergy test, plasmid extraction, polymerase chain reaction, and conjugation experiment.ResultsThirty-five (25.9%) Salmonella isolates were identified from group A, of which 74.3% were S. typhi, 22.9% were S. paratyphi and two (5.7%) were invasive non-typhoidal S. enteritidis. Nine Plasmodium falciparum infections were recorded, four of which were identified as co-infections with typhoidal Salmonella. Only two (8.7%) S. enteritidis samples were obtained from group B (P>0.05). A total of 24 isolates were ESBL-positive, eliciting resistance to five to seven antibiotics, and were multiple-drug resistant. ESBL production due to the blaCTX-M-I gene cluster was detected in eleven (45.8%) Salmonella isolates. Nine (81.8%) of the eleven blaCTX-M-I ESBL producers were S. typhi and two (18.2%) isolates were S. enteritidis. Four of nine S. typhi blaCTX-M-I ESBL-producing strains harbored 23 kb self-transmissible plasmid that was co-transferred with cefotaxime and augmentin resistance to Escherichia coli j53-2 transconjugants.ConclusionThis study revealed the emergence of blaCTX-M-I S. typhi as an agent of persistent pyrexia with potential to spread to other Enterobacteriaceae in Lagos, Nigeria. Cautionary prescription and judicious use of third-generation cephalosporins, particularly cefotaxime, for the treatment of typhoid fever and routine screening for P. falciparum co-infection with ESBL-producing Salmonella in the laboratories during diagnosis of persistent pyrexia conditions in patients are recommended.

show abstract

“…The strains were recovered from the following origins (the number of strains is in parentheses): chickens (11), beef (5), sheep (2), and a turkey (1). They harbored different variants of ESBL genes (number of strains), including (1). and blashv-5 (1) ( Table 1).…”

Section: Methodsmentioning

confidence: 99%

Lineages and Virulence Gene Content among Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains of Food Origin in Tunisia

Jouini

Slama²,

Klibi³

et al. 2013

Journal of Food Protection

View full text Add to dashboard Cite

Nineteen extended-spectrum β-lactamase (ESBL)-positive Escherichia coli strains recovered from food samples in Tunisia were characterized by multilocus sequence typing and phylogenetic typing, and the virulence gene and plasmid content were also determined. These strains presented unrelated pulsed-field gel electrophoresis patterns and contained genes coding for the following ESBLs (the number of strains is in parentheses): CTX-M-1 (15), CTX-M-14 (2), CTX-M-8 (1), and SHV-5 (1). Twelve different sequence types (STs) were identified among the 19 ESBL-positive strains, which included two new STs (ST2022 in 2 bla(CTX-M-14)-containing strains and ST1970 in 2 bla(CTX-M-1)-containing strains). ST155 and ST602 were detected in four and three bla(CTX-M-1)-containing strains, respectively, and ST405 was detected in one bla(CTX-M-8)-producing strain. All ESBL-positive strains were ascribed to the phylogenetic groups A and B1. Most of the bla(CTX-M-1)-containing strains harbored an IncI1 plasmid, except for the four bla(CTX-M-1)-positive strains of beef origin and ST155, which harbored an IncN plasmid. The two bla(CTX-M-14)-containing strains contained an IncI1 plasmid. The virulence gene fimA was detected in all strains. Most strains also carried the aer gene, and six strains carried the eae gene. All strains were negative for the virulence genes sxt, papG-III, papC, hly, cnf1, and bfp. We conclude that ESBL-producing E. coli strains of food origin in Tunisia show high diversity and that plasmids harboring ESBL genes could be implicated in the dissemination of this resistance phenotype.

show abstract

First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla _CTX-M-14 in South America

Cited by 23 publications

References 28 publications

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

blaCTX-M-I group extended spectrum beta lactamase-producing Salmonella typhi from hospitalized patients in Lagos, Nigeria

Lineages and Virulence Gene Content among Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains of Food Origin in Tunisia

Contact Info

Product

Resources

About

First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla CTX-M-14 in South America

Cited by 23 publications

References 28 publications

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

blaCTX-M-I group extended spectrum beta lactamase-producing Salmonella typhi from hospitalized patients in Lagos, Nigeria

Lineages and Virulence Gene Content among Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains of Food Origin in Tunisia

Contact Info

Product

Resources

About

First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla _CTX-M-14 in South America