First identification and multilocus genotyping of Giardia duodenalis in pet chipmunks (Eutamias asiaticus) in Sichuan Province, southwestern China

Deng, Lei; Luo, Run; Liu, Haifeng; Zhou, Ziyao; Li, Lingyu; Chai, Yijun; Yang, Leli; Wang, Wuyou; Fu, Hualin; Zhong, Zhijun; Cao, Suizhong; Peng, Guangneng

doi:10.1186/s13071-018-2790-z

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…To address this point, a large number of epidemiological investigations should be implemented in future studies. However, assemblage B could be transmitted zoonotically [ 70 – 72 ] and has a broad host range, including livestock, companion animals, fish, and marine and wild mammals [ 12 , 13 , 18 , 21 , 26 , 73 , 74 ]. Furthermore, assemblage B was also detected in some water bodies [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…Although the prevalence of G. duodenalis in bamboo rats was, until now, unknown, the presence of this parasite in other rodents (e.g. rats, mice, rabbits, chipmunks, chinchillas, guinea pigs, beavers) has been reported in China, Romania, Laos, America, Germany, Austria, Denmark, Hungary, Finland, France, Italy, Luxembourg, the Netherlands, Norway, Poland, Portugal and Sweden, with a prevalence of 1.9–83.0% [ 9 , 13 , 14 , 36 , 38 – 42 ]. Among them, high prevalence was detected in chinchillas (55.7%) from Romania [ 14 ], chinchillas (61.4%) from European countries [ 42 ] and mice (83%) from Germany [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

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First report of Giardia duodenalis infection in bamboo rats

Wang

Zhang

et al. 2018

Parasites Vectors

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BackgroundThe zoonotic parasite, Giardia duodenalis (syns. G. lamblia and G. intestinalis), has been widely reported in humans and animals, including rodents. The bamboo rat, a rodent species belonged to the subfamily Rhizomyinae, is farmed in China because of its medicinal and edible values. However, no information of G. duodenalis infection was available in bamboo rats prior to the present study. Here, the prevalence and genetic diversity of G. duodenalis in bamboo rats from Hunan Province of China were investigated.ResultsOf 480 faecal samples collected from six farms located in four cities (Wugang, Chenzhou, Huaihua and Jishou) of Hunan Province, 52 (10.8%) were positive for G. duodenalis infection by using a nested PCR approach targeting the beta giardin (bg) gene. Significant differences (P < 0.01) in prevalence were found among different age groups and geographical localities, and among different farms in Wugang city. Sequence analysis revealed existence of the zoonotic assemblage B and genetic diversity of G. duodenalis in these animals. Multilocus genotyping analysis also indicated broad genetic diversity of assemblage B isolates in these bamboo rats.ConclusionsThis is the first report of the infection and genetic variations of G. duodenalis in bamboo rats. These findings will provide basic data for implementing effective strategies to control giardiasis in bamboo rats.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3111-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First report of Giardia duodenalis infection in bamboo rats

Wang

Zhang

et al. 2018

Parasites Vectors

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“…in these animal hosts has been published [ 6 , 7 , 15 , 16 ]. Reports have demonstrated that G. duodenalis infections in rodents in Australia, Belgium, China, Croatia, Germany, Malaysia, Poland, Romania, Spain, Sweden, and the USA ( Table 4 ) have prevalence rates ranging from 1.2% to 100% [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Herein, molecular analysis of 308 fecal samples collected from coypus in six provinces in China confirmed a G. duodenalis prevalence of 12.3% (38/308).…”

Section: Discussionmentioning

confidence: 99%

“…have been detected in rodents, including G. muris and G. microti [ 20 , 35 ]. To date, in China, G. duodenalis has been reported in rodents of several genera, including Chinchillas ( Chinchilla lanigera ), Asian house rats ( Rattus tanezumi ), brown rats ( Rattus norvegicus ), house mice ( Mus musculus ), chipmunks ( Eutamias asiaticus ), and bamboo rats ( Rhizomys sinensis ) [ 18 , 19 , 26 , 30 ]. In previous work, seven assemblages of G. duodenalis were found in rodents, including A, B, C, D, E, F, and G ( Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Multi-Locus Analysis of Giardia duodenalis in Coypus (Myocastor coypus) in China

Cui

Wang

et al. 2021

Pathogens

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Giardia duodenalis is a major gastrointestinal parasite found globally in both humans and animals. This work examined the occurrence of G. duodenalis in coypus (Myocastor coypus) in China. Multi-locus analysis was conducted to evaluate the level of genetic variation and the potential zoonotic role of the isolates. In total, 308 fecal samples were collected from seven farms in China and subjected to PCR screening to reveal G. duodenalis. Notably, G. duodenalis was detected in 38 (12.3%) specimens from assemblages A (n = 2) and B (n = 36). Positive samples were further characterized by PCR and nucleotide sequencing of the triose phosphate isomerase (tpi), beta giardin (bg), and glutamate dehydrogenase (gdh) genes. Multi-locus genotyping yielded 10 novel multi-locus genotypes (MLGs) (one MLG and nine MLGs for assemblages A and B, respectively). Based on the generated phylogenetic tree, AI–novel 1 clustered more closely with MLG AI-2. Furthermore, within the assemblage B phylogenetic analysis, the novel assemblage B MLGs were identified as BIV and clustered in the MLG BIV branch. This is the first report of G. duodenalis in coypus in China. The presence of zoonotic genotypes and subtypes of G. duodenalis in coypus suggests that these animals can transmit human giardiasis.

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“…China: house mouse (3.2%, 1/31); Asian house rat (6.1%, 2/33); brown rat (6.6%, 11/168 and 9.3%, 33/355); pet chipmunks (8.6%, 24/279); bamboo rat (10.8%, 52/480); coypus (12.3%, 38/308); pet chinchillas (27.1%, 38/140) [26,38,[54][55][56][57] (Table 3).…”

Section: Author Contributionsmentioning

confidence: 99%

Genotyping and subtyping of Cryptosporidium spp. and Giardia duodenalis isolates from two wild rodent species in Gansu Province, China

Liu

Jiang

et al. 2021

Preprint

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Cryptosporidium spp. and Giardia duodenalis are common detected intestinal protozoa species in humans and animals, contributing to global gastroenteritis spread. In the present study, we examined the prevalence and zoonotic potential of Cryptosporidium spp. and G. duodenalis in two rodent species in Qinghai-Tibetan Plateau area (QTPA) of China for the first time. A total of 498 intestinal content samples were collected from five counties of QTPA of Gansu province, China. Cryptosporidium spp. and G. duodenalis were found in 2.5% (10/399) and 1.5% (6/399) of Himalayan marmots, while in 1.0% (1/99) and 2.0% (2/99) of Alashan ground squirrels, respectively. Four Cryptosporidium genotypes were identified, including one known horse genotype (n=1) and three novel genotypes designated as marmot genotype I (n = 7), marmot genotype II (n = 2) and marmot genotype III (n = 1). The horse genotype was further subtyped as novel subtype VIbA10. G. duodenalis zoonotic assemblages A (n = 1), B (n = 6), E (n = 1) were identified in the present study. This is the first study to identify Cryptosporidium spp. and G. duodenalis in the two wild rodent species worldwide, suggesting the potential zoonotic transmission of the two pathogens in QTPA.

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First identification and multilocus genotyping of Giardia duodenalis in pet chipmunks (Eutamias asiaticus) in Sichuan Province, southwestern China

Cited by 11 publications

References 32 publications

First report of Giardia duodenalis infection in bamboo rats

First report of Giardia duodenalis infection in bamboo rats

Occurrence and Multi-Locus Analysis of Giardia duodenalis in Coypus (Myocastor coypus) in China

Genotyping and subtyping of Cryptosporidium spp. and Giardia duodenalis isolates from two wild rodent species in Gansu Province, China

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