First identification of canine adenovirus 1 in mink and bioinformatics analysis of its 100 K protein

Abstract

IntroductionAnimal trade favors the spreading of emerging canine adenovirus 1 (CAdV-1) in mink. Because the 100K protein is not exposed to the viral surface at any stage, it can be used to differentiate the vaccine from wild virus infection. However, no related research has been conducted. This study aimed to find evidence of CAdV-1 in mink and predict the character of the 100K protein in the current circulating CAdV-1 strain of mink.MethodIn this experiment, the identification of CAdV-1, the phylogenetic tree, homology, and bioinformatics analysis of 100K were conducted.ResultsThe results showed that the CAdV-1 was identified in the mink and that its Fiber was located in a separate branch. It was closely related to strains isolated from Norwegian Arctic fox and Red fox. 100K was located in a separate branch, which had the closest genetic relationship with skunks, porcupines, raccoons, and hedgehogs and a far genetic relationship with the strains in dogs. 100K protein is an unstable and hydrophobic protein. It had evidence of selective pressure and recombination, 1 glycosylation site, 48 phosphorylation sites, 60 dominant B cell epitopes, and 9 peptides of MHC-I and MHC-II. Its subcellular localization was mainly in the endoplasmic reticulum and mitochondria. The binding sites of 100K proteins were DBP proteins and 33K proteins.DiscussionThe stains in the mink were different from fox. The exploration of its genomic characteristics will provide us with a deeper understanding of the prevention of canine adenovirus.

A Novel Mastadenovirus from <em>N. noctula</em> which Represents a Distinct Evolutionary Branch of Viruses Infecting Bats in Europe

“…The Canine adenoviruses (CAdVs) could infect a wide range of carnivores such as domestic dogs, wolves, bears, foxes, mink, etc. (Balboni et al, 2019(Balboni et al, , 2013Hou et al, 2023; Oleaga, Balseiro, Espí, & Royo, 2022), while some Human adenoviruses (HAdVs) have been detected in monkeys (long-tailed macaques, stump-tailed macaques and great apes) (Kosoltanapiwat et al, 2022;Medkour et al, 2020). The latter supports the view that adenoviruses could be transmitted from primates to humans or vice versa (Chen et Medkour et al, 2020).…”