First identification of porcine parvovirus 6 in Poland

Cui, Jin; Fan, Juan; Gerber, Priscilla F.; Biernacka, Kinga; Stadejek, Tomasz; Xiao, Chao-Ting; Opriessnig, Tanja

doi:10.1007/s11262-016-1386-y

Cited by 28 publications

(21 citation statements)

References 8 publications

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“…PPV5 and PPV6 represent very recently identified PPV genotypes in China and the USA (Xiao et al., ; Ni et al., ; Wu et al., ; Schirtzinger et al., ), but knowledge of their circulating in Europe is still limited (Cui et al., ; Fan et al., ). In Poland, the overall prevalence rate of PPV5 was only 4.0%.…”

Section: Discussionmentioning

confidence: 99%

“…Duplex (PPV1 and PPV2) and triplex (PPV3, PPV4 and PPV5) differential real‐time PCR assays were performed as described (Opriessnig et al., ). In addition, a real‐time PCR assay for PPV6 was performed as described (Cui et al., ). Amplifications were carried out on a 7500 Fast Real‐time PCR system (Applied Biosystems) under the following conditions: initial denaturation at 95°C for 2 min, followed by 40 cycles of 95°C for 15 s and 60°C for 30 s. Samples with cycle threshold (Ct) values over 37 were considered negative.…”

Section: Methodsmentioning

confidence: 99%

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Circulation of Porcine Parvovirus Types 1 through 6 in Serum Samples Obtained from Six Commercial Polish Pig Farms

Cui

Biernacka

Fan

et al. 2016

Transbound Emerg Dis

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Porcine parvoviruses are small non-enveloped DNA viruses, very resistant to inactivation, and ubiquitous in the global pig population. Porcine parvovirus type 1 (PPV1) has been known since the 1960s and is a major causative agent of reproductive failure in breeding herds. During the last decade, several new parvoviruses have been identified in pigs by molecular methods and have been consecutively designated as PPV2 through PPV6. Epidemiology data for these viruses are limited, and the impact of these newly recognized parvoviruses on pigs is largely unknown. To further generate knowledge on the distribution of PPVs in pigs, a total of 247 serum samples were collected from six commercial Polish pig farms during 2013-2015 and tested by PCR assays and ELISAs. The pigs ranged from two to 18 weeks of age at sample collection. Breeding herds supplying the investigated farms were routinely vaccinated against PPV1. While all growing pig samples were negative for PPV1 DNA, young pigs were frequently negative for PPV1 antibodies and seroconversion to PPV1 was commonly seen at 9-10 weeks of age. The PPV2 antibody detection was highest in young pigs (2-6-week-old) and decreased in older pigs indicating passively acquired antibodies. The DNA prevalence rates in the serum samples analysed were 19% for PPV2, 7.7% for PPV3, 2.4% for PPV4, 4.0% for PPV5 and 6.1% for PPV6. Most PPV DNA-positive samples were identified in 9- to 18-week-old pigs with no obvious association with disease on the farm. All recently emerging PPV genotypes were detected in Polish farms. Similar to previous reports in other pig populations, PPV2 was the most frequent PPV genotype circulating in Poland.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Circulation of Porcine Parvovirus Types 1 through 6 in Serum Samples Obtained from Six Commercial Polish Pig Farms

Cui

Biernacka

Fan

et al. 2016

Transbound Emerg Dis

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“…Three duplex real-time PCR assays were performed to detect PPV1 and PPV2, PPV3 and PPV6, PPV4 and PPV5 using previously published primers and probes [9,32,33,35,36] (Table S2). The amplification was carried out on 6000 Rotor Gene (Qiagen, Hilden, Germany) and each reaction of a total volume of 25.0 µL contained 12.5 µL of a reaction mix (SensiFAST Probe No-ROX, Bioline), 2.0 µL of DNA, 1.0 µL of each 10.0 µM primer, and 0.5 µL of each 10.0 µM probe.…”

Section: Methodsmentioning

confidence: 99%

Detection Patterns of Porcine Parvovirus (PPV) and Novel Porcine Parvoviruses 2 through 6 (PPV2–PPV6) in Polish Swine Farms

Miłek

Woźniak

Guzowska

et al. 2019

Viruses

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Porcine parvovirus (PPV) is a major causative agent in reproductive failure, but in the last two decades many novel porcine parvoviruses were described and designated as porcine parvovirus 2 through 6 (PPV2–PPV6). However, their role for pig health is largely unknown. The aim of this study was to better understand the on-farm prevalence of PPVs in different age groups of pigs, and to assess the diagnostic applicability of testing different diagnostic materials. In total, 271 oral fluids, 1244 serum samples, and 1238 fecal samples were collected from 3–21-week-old pigs from 19 farms, and after pooling by 4–6, tested by real-time PCR. The results showed that PPVs are widely spread in Poland and that the highest detection rates were obtained for oral fluids (ranging from 10.7% (PPV1) to 48.7% (PPV2)). Fattening pigs were the age group with the most frequent detection of PPVs (ranging from 8.6% (PPV1) to 49.1% (PPV2)). Porcine parvoviruses were detected mostly in growing-finishing pigs and the infection persisted until the late fattening period, which may suggest the chronic character of the infection (especially for PPV2, which was found to commonly infect animals of all ages). Particularly low Ct values detected for PPV2, PPV3, PPV5, and PPV6 in serum pools from some farms suggested that these viruses may cause high levels of viremia in one or more individuals included in these pools. Further studies are needed to quantify the levels of PPVs viremia and to assess the impact in co-infections with other, often endemic pig viruses, such as porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV).

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“…PPV6 was first isolated in Chinain 2014 [8] and similar to PPV4 [13,14]. Furthermore, PPV7 was isolated in China since 2017.…”

Section: Introductionmentioning

confidence: 97%

Two novel protein chips for the detection of antibodies against porcine parvovirus

Hou

et al. 2020

BMC Vet Res

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Background: PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complementfixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies. Results: We establish the visible protein chip and the cyanine dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein VP2 of PPV was expressed in E.coli, purified with nickel magnetic beads, and then printed onto epoxy-coated glass slides for preparation of the protein chip. After a series of experiments, the conditions of antigen protein concentration, incubation time of primary antibody or secondary antibody, and optimal serum dilution fold were optimized, resulting in a successful visible protein chip and Cy3labeled protein chip. The results showed that the positive serum, diluted up to 6000-fold, can be detected by the visible protein chip, and the positive serum, diluted up to 12,800-fold, can be detected by the Cy3-labeled protein chip, suggesting the high sensitivity of these protein chips. Moreover, the positive detection ratio, sensitivity, and specificity of these two kinds of protein chips were higher than those of commercial ELISA antibody detection kits. Conclusion: Overall, these two protein chips can be used to rapidly diagnose clinical samples with high throughput.

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First identification of porcine parvovirus 6 in Poland

Cited by 28 publications

References 8 publications

Circulation of Porcine Parvovirus Types 1 through 6 in Serum Samples Obtained from Six Commercial Polish Pig Farms

Circulation of Porcine Parvovirus Types 1 through 6 in Serum Samples Obtained from Six Commercial Polish Pig Farms

Detection Patterns of Porcine Parvovirus (PPV) and Novel Porcine Parvoviruses 2 through 6 (PPV2–PPV6) in Polish Swine Farms

Two novel protein chips for the detection of antibodies against porcine parvovirus

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