First-in-human results of an in-body tissue architecture-induced tissue-engineered vascular graft “Biotube” for application in distal bypass for chronic limb-threatening ischemia

Higashita, Ryuji; Miyazaki, Mutsuko; Oi, Masaya; Ishikawa, Noboru

doi:10.1016/j.jvscit.2022.07.007

Cited by 9 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Particularly, we successfully produced cartilage rings with a diameter of more than 1.5 cm, which is equivalent to that of the trachea of a human child ( Supplementary Information ). Additionally, the fabrication of the iBTA tissues, tube-like tissues with diameters ranging from 0.6 to 20 mm which are applicable as artificial blood vessels 13 , 25 , can be easily fabricated by changing the mold size. Therefore, we believe that the proposed tracheal analogues in this study can be scaled up without losing their functionality.…”

Section: Discussionmentioning

confidence: 99%

“…However, it was difficult to control the shape of such scaffold gels based on in vivo tissue engineering techniques. In contrast, the iBTA technique has been used in generating tissues of various 3D shapes with complicated semicircular pocket structures, such as tube, sheet, and heart valve-like tissues 13 – 17 . In this study, the main component of the iBTA tissues is the connective tissue consisting of fibroblasts, collagen, and capillary vessels.…”

Section: Discussionmentioning

confidence: 99%

“…In a separate study, we developed an in vivo tissue engineering technique called the in-body tissue architecture (iBTA). We prepared iBTA-induced tissues of various shapes and sizes, such as a tubular tissue called Biotube for vascular grafts 12 , 13 , sheet-like tissue called Biosheet for cornea 14 , aortic valve leaflets 15 , myocardium 16 , and others, and a more complex heart valve-like tissue called Biovalve 17 . The Biotube has already been clinically applied as an alternative blood vessel for bypass to a chronic limb-threating ischemia patient 13 or hemodialysis patients.…”

Section: Introductionmentioning

confidence: 99%

“…We prepared iBTA-induced tissues of various shapes and sizes, such as a tubular tissue called Biotube for vascular grafts 12 , 13 , sheet-like tissue called Biosheet for cornea 14 , aortic valve leaflets 15 , myocardium 16 , and others, and a more complex heart valve-like tissue called Biovalve 17 . The Biotube has already been clinically applied as an alternative blood vessel for bypass to a chronic limb-threating ischemia patient 13 or hemodialysis patients. The patch implantation of the Biosheets into the partial tracheal defects in beagle dogs resulted in the reconstruction of the tracheal tissue by covering the ciliated epithelium on the lumen surface and the partial infiltration of chondrocyte aggregates in the middle wall 18 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports

Hiwatashi

Iwai

Nakayama³

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Tracheas have a tubular structure consisting of cartilage rings continuously joined by a connective tissue membrane comprising a capillary network for tissue survival. Several tissue engineering efforts have been devoted to the design of scaffolds to produce complex structures. In this study, we successfully fabricated an artificial materials-free autologous tracheal analogue with engraftment ability by combining in vitro cell self-aggregation technique and in-body tissue architecture. The cartilage rings prepared by aggregating chondrocytes on designated culture grooves that induce cell self-aggregation were alternately connected to the connective tissues to form tubular tracheal analogues by subcutaneous embedding as in-body tissue architecture. The tracheal analogues allogeneically implanted into the rat trachea matured into native-like tracheal tissue by covering of luminal surfaces by the ciliated epithelium with mucus-producing goblet cells within eight months after implantation, while maintaining their structural integrity. Such autologous tracheal analogues would provide a foundation for further clinical research on the application of tissue-engineered tracheas to ensure their long-term functionality.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports

Hiwatashi

Iwai

Nakayama³

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…One recent approach is embedding a biotube in subcutaneous spaces of a patient for months, using patient’s body to fabricate a graft. This has been used successfully as a pulmonary artery substitute in pediatric patients ( Kato et al, 2016 ; Fujita et al, 2020 ; Nakatsuji et al, 2021 ; Higashita et al, 2022 ). Another approach was employing biodegradable scaffolds in combination with autologous bone marrow mononuclear cells.…”

Section: Translation Of Vascular Grafts For Clinics: Success Measuresmentioning

confidence: 99%

Strategies to counteract adverse remodeling of vascular graft: A 3D view of current graft innovations

Tan

Boodagh

Parthiban

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Vascular grafts are widely used for vascular surgeries, to bypass a diseased artery or function as a vascular access for hemodialysis. Bioengineered or tissue-engineered vascular grafts have long been envisioned to take the place of bioinert synthetic grafts and even vein grafts under certain clinical circumstances. However, host responses to a graft device induce adverse remodeling, to varied degrees depending on the graft property and host’s developmental and health conditions. This in turn leads to invention or failure. Herein, we have mapped out the relationship between the design constraints and outcomes for vascular grafts, by analyzing impairment factors involved in the adverse graft remodeling. Strategies to tackle these impairment factors and counteract adverse healing are then summarized by outlining the research landscape of graft innovations in three dimensions—cell technology, scaffold technology and graft translation. Such a comprehensive view of cell and scaffold technological innovations in the translational context may benefit the future advancements in vascular grafts. From this perspective, we conclude the review with recommendations for future design endeavors.

show abstract

Bladder Reconstruction in Cats Using in Body Tissue Architecture (iBTA)-Induced Biosheet

Fujita,

Sugiyama,

Tsuboi

et al. 2024

Preprint

View full text Add to dashboard Cite

Urinary tract diseases are common in cats, and often require surgical reconstruction. Here, to explore the possibility of urinary tract reconstruction in cats using in-vivo tissue architecture (iBTA), biosheets fabricated using iBTA technology were implanted into the feline bladder and the regeneration process was histologically evaluated. The biosheets were prepared by embedding molds into the dorsal subcutaneous pouches of six cats for 2 months. A section of the bladder wall was removed, and the biosheets were sutured to the excision site. After 1 and 3 months of implantation, the biosheets were harvested and evaluated histologically. Implantable biosheets were formed with a success rate of 67%. There were no major complications following implantation, including tissue rejection, severe inflammation, or infection. Urinary incontinence was also not observed. Histological evaluation revealed the bladder lumen was almost entirely covered by urothelium after 1 month, with myofibroblast infiltration into the biosheets. After 3 months, the urothelium became multilayered, and mature myocytes and nerve fibers were observed at the implantation site. In conclusion, this study showed that tissue reconstruction using iBTA can be applied to cats, and that biosheets have the potential to be useful in both the structural and functional regeneration of the feline urinary tract.

show abstract

First-in-human results of an in-body tissue architecture-induced tissue-engineered vascular graft “Biotube” for application in distal bypass for chronic limb-threatening ischemia

Cited by 9 publications

References 11 publications

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports

Strategies to counteract adverse remodeling of vascular graft: A 3D view of current graft innovations

Bladder Reconstruction in Cats Using in Body Tissue Architecture (iBTA)-Induced Biosheet

Contact Info

Product

Resources

About