First in vitro and in vivo results of an anti-human CD133-antibody coated coronary stent in the porcine model

Sedaghat, Alexander; Sinning, Jan‐Malte; Paul, Kathrin; Kirfel, Gregor; Nickenig, Georg; Werner, Nikos

doi:10.1007/s00392-013-0547-4

Cited by 34 publications

(20 citation statements)

References 64 publications

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“…Although these inert, stiff polymeric grafts successfully replace large arteries, problems arise with small-diameter vessels resulting in high stenosis rates [3]. Major risk factors leading to stenosis include unsatisfactory biocompatibility [4] and lack of endothelialization capacity [5]. In the more recent tissue engineering approach, cells are induced to produce collagen, elastin, or other matrix components.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Study of a Novel Nanogold-Collagen Composite to Enhance the Mesenchymal Stem Cell Behavior for Vascular Regeneration

Hung

Chang

et al. 2014

PLoS ONE

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Novel nanocomposites based on type I collagen (Col) containing a small amount (17.4, 43.5, and 174 ppm) of gold nanoparticles (AuNPs, approximately 5 nm) were prepared in this study. The pure Col and Col-AuNP composites (Col-Au) were characterized by the UV-Vis spectroscopy (UV-Vis), surface-enhanced raman spectroscopy (SERS) and atomic force microscopy (AFM). The interaction between Col and AuNPs was confirmed by infrared (IR) spectra. The effect of AuNPs on the biocompatibility of Col, evaluated by the proliferation and reactive oxygen species (ROS) production of mesenchymal stem cells (MSCs) as well as the activation of monocytes and platelets, was investigated. Results showed that Col-Au had better biocompatibility than Col. Upon stimulation by vascular endothelial growth factor (VEGF) and stromal derived factor-1α (SDF-1α), MSCs expressed the highest levels of αvβ3 integrin/CXCR4, focal adhesion kinase (FAK), matrix metalloproteinase-2 (MMP-2), and Akt/endothelial nitric oxide synthase (eNOS) proteins when grown on the Col-Au (43.5 ppm) nanocomposite. Taken together, Col-Au nanocomposites may promote the proliferation and migration of MSCs and stimulate the endothelial cell differentiation. These results suggest that Col-Au may be used to construct tissue engineering scaffolds for vascular regeneration.

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Section: Introductionmentioning

confidence: 99%

In Vitro Study of a Novel Nanogold-Collagen Composite to Enhance the Mesenchymal Stem Cell Behavior for Vascular Regeneration

Hung

Chang

et al. 2014

PLoS ONE

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“…In other mature blood cells, such as nucleated red blood cells, lymphocytes, myelocytic cells, mononuclear cells, and platelets, CD133 expressions were not detected. Therefore CD133 is the more specific choice for the preparation of EPCs capture stents [18]. …”

Section: Introductionmentioning

confidence: 99%

Anti-CD133 Antibody Immobilized on the Surface of Stents Enhances Endothelialization

Gong

et al. 2014

BioMed Research International

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Drug eluting stents successfully reduce restenosis at the cost of delayed reendothelialization. In recent years, a novel concept to enhance reendothelialization using anti-CD34 antibody coated stents which capture circulating progenitor cells (EPCs) has been developed with conflicting clinical results. CD133 is a glycoprotein expressed on circulating hematopoietic and putative endothelial-regenerating cells and may be superior to CD34 for EPCs capture stents. In the present study, anti-CD133 antibody has been successfully immobilized to the biodegradable polymeric coating material by covalent conjugation. We explore whether anti-CD133 antibody coated stents (CD133 stents) might accelerate reendothelialization in comparison with bare metal stents (BMS) through the superior ability to capture EPCs. The in vitro cell culture results indicate that anti-CD133 antibody functionalized polymer film significantly promotes CD133 positive cells attachment and growth compared with the unfunctionalized polymer film. In the semi-in vivo arteriovenous shunt model CD133 stents demonstrate much quicker specific capturing of EPCs from the blood stream than BMS within 6 hours. In a porcine coronary artery injury model CD133 stents show more effective reendothelialization in short term compared with BMS, while no significant difference in endothelial function recovery was observed between these two groups within 6-month followup.

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“…Furthermore, many researches have performed studies with bone marrow-derived progenitor cells and circulating progenitor cells concerning its impact to postinfarction heart function. There have observed inconsistent effects on LV remodeling [9, 12, 20, 24]. …”

Section: Introductionmentioning

confidence: 99%

Characteristic of c-Kit+ progenitor cells in explanted human hearts

et al. 2014

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According to literature data, self-renewing, multipotent, and clonogenic cardiac c-Kit+ progenitor cells occur within human myocardium. The aim of this study was to isolate and characterize c-Kit+ progenitor cells from explanted human hearts. Experimental material was obtained from 19 adult and 7 pediatric patients. Successful isolation and culture was achieved for 95 samples (84.1 %) derived from five different regions of the heart: right and left ventricles, atrium, intraventricular septum, and apex. The average percentage of c-Kit+ cells, as assessed by FACS, ranged between 0.7 and 0.9 %. In contrast to published data we do not observed statistically significant differences in the number of c-Kit+ cells between disease-specific groups, parts of the heart or sexes. Nevertheless, c-Kit+ cells were present in significant numbers (11–24 %) in samples derived from three explanted pediatric hearts. c-Kit+ cells were also positive for CD105 and a majority of them was positive for CD31 and CD34 (83.7 ± 8.6 and 75.7 ± 11.4 %, respectively). Immunohistochemical analysis of the heart tissue revealed that most cells possessing the c-Kit antigen were also positive for tryptase, a specific mast cell marker. However, flow cytometry analysis has shown cultured c-Kit+ cells to be negative for hematopoietic marker CD45 and mast cell marker CD33. Isolated c-Kit+ cells display mesenchymal stem cell features and are thought to differentiate into endothelial cells.

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First in vitro and in vivo results of an anti-human CD133-antibody coated coronary stent in the porcine model

Cited by 34 publications

References 64 publications

In Vitro Study of a Novel Nanogold-Collagen Composite to Enhance the Mesenchymal Stem Cell Behavior for Vascular Regeneration

In Vitro Study of a Novel Nanogold-Collagen Composite to Enhance the Mesenchymal Stem Cell Behavior for Vascular Regeneration

Anti-CD133 Antibody Immobilized on the Surface of Stents Enhances Endothelialization

Characteristic of c-Kit+ progenitor cells in explanted human hearts

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