First influenza D virus full-genome sequence retrieved from livestock in Namibia, Africa

Molini, Umberto; Curini, Valentina; Jacobs, Eugene A.; Tongo, Eesie; Berjaoui, Shadia; Hemberger, Maria Yvonne; Puglia, Ilaria; Jago, Mark; Khaiseb, Siegfried; Cattoli, Giovanni; Dundon, William G.; Lorusso, Alessio; Giallonardo, Francesca Di

doi:10.1016/j.actatropica.2022.106482

Cited by 13 publications

(13 citation statements)

References 28 publications

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“…A previous study done in the Southern African country of Namibia by Molini et al (2022) showed a molecular prevalence of 3.1% from 65 cattle which is far low when compared to the current study. This study was also higher than the prevalence of 22.8% reported elsewhere in Canada by Zhang et al (2019); 19% in China by Zhai et al (2017); 10% in the USA by Huang et al (2021), 8.7% reported in the UK by Dane et al (2019), 5.6% in Ireland by Flynn et al (2018), 4.5% reported from France by Ducatez et al (2015) , 3.9% in Turkey by Yilmaz et al (2020) and the lowest 1.3% documented in Italy by Chiapponi et al (2019).…”

Section: Discussioncontrasting

confidence: 91%

“…In Asia, 12.8% IDV was detected from 156 dairy cattle and 7.3% from 55 native yellow cattle in China (Zhai et al, 2017). Limited studies in Africa revealed 3.07% incidence rate of IDV from 2 positive nasal swab samples out of 65 cattle tested in Namibia (Molini et al, 2022). Therefore, to ameliorate the possible challenge of epizootic with the potential zoonotic transmission based on the 1.3% seroprevalence of IDV in the general human population (Hause et al, 2013), there is a need for continuous surveillance of IDV for proper documentation that will inform the government including the policy-makers on the need for more preparedness at the animal or reservoir host interface, especially in a country like Nigeria with over 20 million cattle per head that are reared indiscriminately in the backyard and commercial farms.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First Molecular Detection of Influenza D virus in Cattle from Commercial farm in Nigeria, Sub-Saharan Africa

Anjorin¹,

Moronkeji²,

Temenu³

et al. 2023

Preprint

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Influenza D virus (IDV) was first reported in pigs in the USA, and since then the virus has become a public health issue. In Nigeria, no work has been done on IDV despite the manifestation of influenza-like illness in cattle. This study aimed at molecular surveillance of IDV in cattle in Lagos. Prospective epidemiological investigation was initiated in a large commercial farm market where animals in open pens are reared, sold, and butchered under poor hygienic conditions without adequate biosecurity measures. A total of 80 nasopharyngeal swabs were collected between October and November 2021. The samples were extracted using an RNA purification kit (NIMR). RNA extracts were amplified following a two-step PCR using FIREScript RT cDNA synthesis kit (Solis Biodyne, Estonia), followed by PCR OneTaq Quick-load 2x master-mix (NEB, UK) in a Rotor-Gene thermocycler (Qiagen, Germany). Amplicons were detected using a 1.5% Gel electrophoresis. IDV was detected in 26/80 (32.5%) cattle. Sick animals showed 65% (17/26) almost double burden of IDV higher than 34.6% (9/26) in a healthy population, including 88.2% (15/17) Cattle with diarrhoea and 11.8% (2/17) with nausea having IDV. Bull recorded more than twice, 18/26 (69.2%) incidence by gender compared to Cow. Age prevalence showed 62.23% (18/26) highest detection in cattle of 4 years old, followed by 23.07% (6/26) in 5 years old, while the lowest 7.69% (2/26) was recorded in 3 years old. This study showed the first molecular detection of IDV in Nigeria and West Africa sub-region to the best of our knowledge. It underscores the need for continuous surveillance of IDV at the animal-human interface.

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Section: Discussioncontrasting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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First Molecular Detection of Influenza D virus in Cattle from Commercial farm in Nigeria, Sub-Saharan Africa

Anjorin¹,

Moronkeji²,

Temenu³

et al. 2023

Preprint

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“…IDV was first isolated in 2011 from pigs in Oklahoma [ 2 ]. Since then, IDV has been isolated/detected from cattle across many countries, including the United States (US), Mexico [ 3 ], Ireland [ 4 ], United Kingdom [ 5 ], France [ 6 ], Italy [ 7 ], China [ 8 ], Canada [ 9 ], Japan [ 10 ], Turkey [ 11 ], Netherlands [ 12 ], Brazil [ 13 ], South Korea [ 14 ], Australia [ 15 ], Argentina [ 16 ], Namibia [ 17 ] and Denmark [ 18 ]. Serological evidence also indicates IDV presence in Morocco, Togo, Benin, Kenya [ 19 ], Luxembourg [ 20 ] and Ethiopia [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

IDV Typer: An Automated Tool for Lineage Typing of Influenza D Viruses Based on Return Time Distribution

Limaye,

Shelke,

Kale

et al. 2024

Viruses

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Influenza D virus (IDV) is the most recent addition to the Orthomyxoviridae family and cattle serve as the primary reservoir. IDV has been implicated in Bovine Respiratory Disease Complex (BRDC), and there is serological evidence of human infection of IDV. Evolutionary changes in the IDV genome have resulted in the expansion of genetic diversity and the emergence of multiple lineages that might expand the host tropism and potentially increase the pathogenicity to animals and humans. Therefore, there is an urgent need for automated, accurate and rapid typing tools for IDV lineage typing. Currently, IDV lineage typing is carried out using BLAST-based searches and alignment-based molecular phylogeny of the hemagglutinin-esterase fusion (HEF) gene sequences, and lineage is assigned to query sequences based on sequence similarity (BLAST search) and proximity to the reference lineages in the tree topology, respectively. To minimize human intervention and lineage typing time, we developed IDV Typer server, implementing alignment-free method based on return time distribution (RTD) of k-mers. Lineages are assigned using HEF gene sequences. The server performs with 100% sensitivity and specificity. The IDV Typer server is the first application of an RTD-based alignment-free method for typing animal viruses.

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“…Influenza D virus (IDV), a new member of the family Orthomyxoviridae, was initially isolated from pigs with respiratory symptoms in the United States in 2011 1 . Further epidemiological studies demonstrated cattle as the primary host 2,3 , and IDVs circulated in cattle in the American [2][3][4][5][6][7][8][9] , European [10][11][12][13][14] , Asian [15][16][17] , and African countries 18,19 . Although IDV infection causes mild to moderate respiratory illnesses in cattle, two metagenomic studies conducted on cattle diagnosed with bovine respiratory disease complex (BRDC) have found a positive correlation between IDV infection and BRDC 5,20 .…”

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confidence: 99%

Generation of a recombinant temperature-sensitive influenza D virus

Ishida

Murakami

Kamiki

et al. 2023

Sci Rep

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Influenza D virus (IDV) is a causative agent of the bovine respiratory disease complex (BRDC), which is the most common and costly disease affecting the cattle industry. For developing a candidate vaccine virus against IDV, we sought to produce a temperature-sensitive strain, similar to the live attenuated, cold-adapted vaccine strain available against the influenza A virus (IAV). To this end, we produced a recombinant IDV (designated rD/OK-AL) strain by introducing mutations responsible for the adaptation of the IAV vaccine strain to cold conditions and conferring sensitivity to high temperatures into PB2 and PB1 proteins using reverse genetics. The rD/OK-AL strain grew efficiently at 33 °C but did not grow at 37 °C in the cell culture, indicating its high-temperature sensitivity. In mice, rD/OK-AL was attenuated following intranasal inoculation. It mediated the production of high levels of antibodies against IDV in the serum. When the rD/OK-AL-inoculated mice were challenged with the wild-type virus, the virus was not detected in respiratory organs after the challenge, indicating complete protection against IDV. These results imply that the rD/OK-AL might be a potential candidate for the development of live attenuated vaccines for IDV that can be used to control BRDC.

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First influenza D virus full-genome sequence retrieved from livestock in Namibia, Africa

Cited by 13 publications

References 28 publications

First Molecular Detection of Influenza D virus in Cattle from Commercial farm in Nigeria, Sub-Saharan Africa

First Molecular Detection of Influenza D virus in Cattle from Commercial farm in Nigeria, Sub-Saharan Africa

IDV Typer: An Automated Tool for Lineage Typing of Influenza D Viruses Based on Return Time Distribution

Generation of a recombinant temperature-sensitive influenza D virus

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