First insight into the effect of single oral dose therapy with artemisinin–naphthoquine phosphate combination in a mouse model of Schistosoma mansoni infection

El-Beshbishi, Samar N.; Taman, Amira; El-Malky, Mohamed; Azab, Manar S; El-Hawary, Amira Kamal; El-Tantawy, Dina

doi:10.1016/j.ijpara.2013.01.007

Cited by 25 publications

(17 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation indicates that SYN can target all the intra-mammalian developmental stages of the parasite. This complies with similar studies on artemesinins tested for anti-schistosomal properties [28][29][30][31].…”

supporting

confidence: 91%

Repurposing of Anti-malarial SynriamTM and testing its efficacy against Egyptian strain of Schistosoma hematobium

Yousof

El-Sayed

Elfar

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Trials for discovering an anti-malarial drug, which can compete Schistosomal infection in co-endemic areas, are ongoing. Some preliminary studies were done on Synriam (SYN), anti-malarial drug combination (arterolane maleate and piperaquine phosphate) released from Ranbaxy, to test its anti-schistosomal effect. However, in vitro incubation of SYN with different Schistosoma hematobium stages was not fully assessed. The aim of this study is to determine the anti-schistosomal in vitro effect of SYN on adult and juvenile stages of Schistosoma hematobium- Egyptian strain.Methods: The adult and juvenile worms were incubated with ascending concentrations of SYN and with praziquantel as positive control. Viability, survival, morphological and ultrastructural changes were assessed at different time points.Results: Higher concentrations of 60, 80 µg/ml showed rapid and lethal effects on adult and juvenile stages of both species, with prominent ultrastructural alterations. Concentrations of (10, 20, 40 µg/ml) showed mild to moderate effect on adult schistosomes. However, contrarily to praziquantel, larval immature stages responded significantly and rapidly to low concentration of SYN with 100% death rate.Conclusion: The present findings are consistent with the evaluation of anti-schistosomal therapeutic effect of SYN to be utilized in malaria co-endemic areas.

show abstract

supporting

confidence: 91%

Repurposing of Anti-malarial SynriamTM and testing its efficacy against Egyptian strain of Schistosoma hematobium

Yousof

El-Sayed

Elfar

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Adult snails (shell diameters 19–25 mm) and egg capsules were employed in bioassay experiments. The BH strain (Belo Horizonte, MG, Brazil) of S. mansoni was routinely maintained at the Laboratório de Imunopatologia Keiko Asami (UFPE) by passage through B. glabrata and mice (El-Beshbishi et al 2013). …”

Section: Methodsmentioning

confidence: 99%

A penta-substituted pyridine alkaloid from the rhizome of Jatropha elliptica (Pohl) Muell. Arg. is active against Schistosoma mansoni and Biomphalaria glabrata

et al. 2014

View full text Add to dashboard Cite

Jatropha elliptica is a shrub distributed throughout the north and west of Brazil and reputedly possesses a wide range of therapeutical properties. The roots of this plant possess molluscicidal activity and contain terpenoids, coumarin, lignoid, steroids and alkaloid. In the present study, we assessed the schistosomicidal, miracicidal and cercaricidal activities (against Schistosoma mansoni) and molluscicidal activities (against adults and egg masses of Biomphalaria glabrata) of the alkaloid diethyl 4-phenyl-2,6-dimethyl-3,5-pyridinedicarboxylate, isolated from the ethanol extract of the rhizome of J. elliptica, have been determined. The alkaloid was 100 % lethal to adult schistosomes within 4 days at a concentration of 50 μg/mL. Alterations were observed in the schistosome tegument occasioned by treatment with the alkaloid, such as formation of vesicles and vacuolisation. The extent of tegumental damage of the worm was proportional to the time of incubation and to the concentration of compound. The alkaloid also exhibited a potent cercaricidal activity (LC100 = 2 μg/mL); it was totally ineffective against miracicidal forms of the parasite. Moreover, the alkaloid presented strong activity against adult snails (LC90 = 36.43 μg/mL) but was inactive against their egg masses. It is observed then the potential of this compound for the development of new therapies for the treatment of schistosomiasis.

show abstract

“…The effective dose values were used to assess the potency of activity of compounds and to monitor the resistance and susceptibility profiles of the parasite strain in relation to the evaluated substance. 8,28,30,31 Adult worms exposed to concentrations of 50.0, 75.0 and 100.0 lg/mL of the EEt maintained normal movement and mating, with no evidence of tegument injury. In addition, egg laying was observed over eight days of experiment.…”

Section: Introductionmentioning

confidence: 95%

In vitro evaluation of the schistosomicidal effect of the extracts, fractions and major 3-hydroxy-2,6-dialkyl-substituted piperidine alkaloids from the flowers of Senna spectabilis (Fabaceae)

Castro

Silva

et al. 2016

Bioorganic & Medicinal Chemistry Letters

View full text Add to dashboard Cite

In this work, we present the in vitro schistosomicidal activity evaluation of the most active dichloromethane fraction (FDm) (ED50=83.5μg/mL) and of a mixture of the major alkaloids ((-)-cassine/(-)-spectaline, C/E) (ED50=37.4μg/mL) from the flowers of Senna spectabilis against adult worms and cercariae. We also demonstrate other toxic effects including paralysis of the adult worms, inhibition of the secretory activity, tegument lesions and cercaricidal activity. In the association test of Praziquantel (PZQ)-C/E, we observed up to 80% mortality of Schistosoma mansoni in comparison to PZQ monotherapy. Due to the diversity of the toxic effects, the schistosomicidal activity of C/E is likely a result of a multitarget mechanism involving the tegument, secretory system and neuromotor action.

show abstract

First insight into the effect of single oral dose therapy with artemisinin–naphthoquine phosphate combination in a mouse model of Schistosoma mansoni infection

Cited by 25 publications

References 45 publications

Repurposing of Anti-malarial SynriamTM and testing its efficacy against Egyptian strain of Schistosoma hematobium

Repurposing of Anti-malarial SynriamTM and testing its efficacy against Egyptian strain of Schistosoma hematobium

A penta-substituted pyridine alkaloid from the rhizome of Jatropha elliptica (Pohl) Muell. Arg. is active against Schistosoma mansoni and Biomphalaria glabrata

In vitro evaluation of the schistosomicidal effect of the extracts, fractions and major 3-hydroxy-2,6-dialkyl-substituted piperidine alkaloids from the flowers of Senna spectabilis (Fabaceae)

Contact Info

Product

Resources

About