First inter-laboratory study of a Supercritical Fluid Chromatography method for the determination of pharmaceutical impurities

Ultra‐high performance supercritical fluid chromatography coupled to tandem mass spectrometry for antidoping analyses: Assessment of the inter‐laboratory reproducibility with urine samples

“…This point must be properly assessed before UHPSFC can be considered as a viable alternative in routine laboratories. Only one work focusing on assessing the inter‐laboratory reproducibility for a UHPSFC method was made, using a UV detector and simple pharmaceutical formulation 15 . In the present work, the robustness of a generic UHPSFC method using complex matrices (biological fluids), as well as an MS detector (MS/MS) hyphenated to the UHPSFC instrument was investigated.…”

“…In the past 3‐5 years, there has been an increasing number of studies focusing on how UHPSFC can guarantee similar performance to UHPLC in terms of method robustness. The comparison has been demonstrated with standard compounds and, more recently, with biological matrices too 8,15,16 . Nonetheless, nothing has been done so far in assessing the robustness of a UHPSFC bioanalytical method across different laboratories.…”

Supercritical fluid chromatography coupled to mass spectrometry for lipidomics

“…It is recommended to set up designed experiments to determine the optimal ionization parameters during method development, especially for targeted approaches where trace quantification may be required. As an interlaboratory study was undertaken in SFC-UV, 43 it would be extremely attractive to carry out the same work with different SFC-MS platforms. Viscosity, h (g.s.cm -1 ) 10 -4 10 -4 10 -2 Chromatographic column is connected directly to MS through a pressure restrictor heated at its end, (b) same as in (a) but here the restrictor is heated at the beginning, (c) Full flow from the column is first passed through a UV detector, and then is mixed with an organic solvent delivered by a fixed-pressure pump, before being expanded to MS through a restrictor, (d) here flow from the column is passed through an automated back-pressure regulator (ABPR) before the MS and the flow is mixed with a make-up pump flow, either before or after the ABPR, (e) here a part of the outlet from the column is directed towards an MS whereas the other part is taken to an ABPR passing through a UV detector, (f) in this configuration, flow from the column is first passed through a UV detector, and then is mixed with an organic solvent delivered by a make-up pump.…”

Supercritical fluid chromatography coupled to mass spectrometry for lipidomics

“…It is recommended to set up designed experiments to determine the optimal ionization parameters during method development, especially for targeted approaches where trace quantification may be required. As an interlaboratory study was undertaken in SFC-UV, 43 it would be extremely attractive to carry out the same work with different SFC-MS platforms.…”