First isolation and characterization of the pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome in the indigenous Pelophylax ridibundus of Al-Ahsaa, Saudi Arabia

Khalifa, Ashraf; Bekhet, Gamal M.

doi:10.1016/j.micpath.2017.10.019

Cited by 19 publications

(17 citation statements)

References 28 publications

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“…Identification of the bacterial strains was carried out using the comparative sequence analysis of the 16S rDNA gene. Genomic DNA extraction, primers used, PCR conditions, and sequencing were performed as previously described (Khalifa and Bekhet 2018).…”

Section: Identification Of Bacterial Strains Using 16s Rrna Sequencingmentioning

confidence: 99%

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

Khalifa¹

2020

SJKFU

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Plant growth-promoting bacteria are present in the rhizosphere, a soil zone around plant roots. An important mechanism of plant growth enhancement is the production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Three ACC deaminase-producing bacterial strains were isolated from the rhizosphere of the halophytic plant Zygophyllum coccineum growing in Al-Uqair, Al-Ahsa, Saudi Arabia. The strains were identified as Bacillus filamentosus, Janibacter indicus, and Brevibacterium casei based on 16S rRNA sequence, and their taxonomic position was determined. Furthermore, the strains exhibited catalase and indoleacetic acid production and phosphate solubilization. The highest ACC deaminase activity was observed in B. filamentosus (~ 449 nmole α-ketobutyrate mg-1 h-1), followed by J. indicus and B. casei (~ 66 nmole α-ketobutyrate mg-1 h-1). Inoculation of wheat grains with each strain significantly enhanced growth. Z. coccineum rhizosphere harbors growth-promoting bacteria and ameliorated the negative effects of salt stress on wheat plants and may be used as an eco-friendly biofertilizer.

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Section: Identification Of Bacterial Strains Using 16s Rrna Sequencingmentioning

confidence: 99%

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

Khalifa¹

2020

SJKFU

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“…Discrete pure colonies of the strain ESM-1 was investigated under scanning electron microscopy (Joel) as previously described (Khalifa and Bekhit, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…In order to identify the strain ESM-1 accurately, 16S rRNA gene sequencing was applied. Extraction of genomic DNA, amplification of the target gene with the universal primers, PCR conditions and sequencing of the purified amplicon were performed as described earlier (Khalifa and Bekhit, 2017).…”

Section: Methodsmentioning

confidence: 99%

Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa

Khalifa

Almalki

2019

Saudi Journal of Biological Sciences

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In this study, one bacterial strain, ESM-1, was isolated from rhizosphere of Eruca sativa, growing in Al Hofouf, Saudia Arabia, after enrichment with methanol as a sole carbon and energy source in a batch culture. ESM-1 was characterized by a polyphasic approach. The strain was identified as Delftia acidovorans at similarity level of 99.9% of the 16S rRNA gene sequences. Results of the Biolog Gen III MicroPlate test system showed that strain ESM-1 reacted positively to 47 (50%) including the one-carbon compound formic acid, and partially positive to 6 (∼6.4%) out of the 94 different the traits examined. The total cellular fatty acids composition of the strain ESM-1 was (C16:1ω7c/C16:1ω6c) and C16:0) and matched that of Delftia acidovorans at a similarity index of 0.9, providing a robustness to the ESM-1 identification. Furthermore, ESM-1 displayed a complex polar lipid profile consisting of phosphatidylethanolamine, phosphatidylglycerol, glycolipid, aminolipid, in addition to uncharacterized lipids. The DNA G+C content of the strain was 66.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain ESM1-1 was clearly clustered within the Delftia clade and constructed a monophyletic subcluster with Delftia acidovorans NBRC14950. The results addressed that ESM-1 is a facultative methylotrophic bacterium indigenous to Al Hofouf region and opens the door for potential biotechnological applications (e.g., bioremediation) of this strain, in future. Additionally, these findings assure that the total cellular fatty acid analysis and 16S rRNA gene are reliable tool for bacterial characterization and identification.

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“…Infections with virulent pathogenic microbes contribute significantly to the large‐scale mortality of amphibians (Scheele et al, 2017). Examples are Chlamydia psittaci which causes chlamydiosis; Batrachochytrium dendrobatidis which causes chytridiomycosis (Bayramova et al., 2018), Aeromonas veronii which causes ulcerative syndrome (Khalifa and Bekhit et al, 2018), Aeromonas, Citrobacter , Proteus and Pseudomonas all can cause red‐leg disease (Hayes et al., 2010). Moreover, potential zoonotic concerns are associated with these microbial species that are a serious challenge to researchers (Pasmans et al., 2017; Patocka et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Pathological and mortality findings associated withAeromonas hydrophilafrom frog eggs in Al‐Ahsa region of Saudi Arabia

2020

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Background Amphibians decline globally due to infection with virulent bacteria, despite their vital roles in the ecosystem. The current study aimed at addressing the pathological and bacterial findings of the infected Pelophylax ridibundus indigenous to the Al‐Ahsa region. Many clinical signs were observed in the eggs, including discoloration, disintegration of the outer jelly coat; defects in morphology, and mortality were reported. Furthermore, neuromasts lacking apical cilia and microvilli were observed in the cleavage stages of the infected developing egg. Additionally, cutaneous hyperaemia, lethargy, peripheral oedema, subcutaneous haemorrhage and cutaneous ulcerations were observed in the infected P. ridibundus adults. Methods Ribbons of frog eggs were collected from Al‐Ahsa oasis, the Eastern region, Kingdom of Saudi Arabia (KSA), and examined under light and scanning electron microscope. The developed eggs and adults were further examined using a dissecting microscope to determine signs of infection. A bacterial strain designated as PRE‐1 was isolated from the infected eggs, identified genotypically and then characterized phenotypically. Strain PRE‐1 was identified as Aeromonas hydrophila based on the comparative gene sequences of the 16S rRNA gene at identity level of (99.92%). Furthermore, phylogenetic relationship based on the 16S rRNA gene sequences of our strain and other relevant strains Aeromonas spp. showed that PRE‐1 formed a cluster with A. hydrophila (99% bootstrap support), providing a robustness to the identification of PRE‐1 as A. hydrophila. This bacterium was found to be associated with disease outbursts in the marsh frogs from the Al‐Ahsa region. Conclusion It is important to continue monitoring the emerging diseases so that their cause can be discerned, and appropriate protocols can be applied to limit the spread of the loss of amphibians, which are vital for our ecosystem.

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First isolation and characterization of the pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome in the indigenous Pelophylax ridibundus of Al-Ahsaa, Saudi Arabia

Cited by 19 publications

References 28 publications

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

Polyphasic characterization of Delftia acidovorans ESM-1, a facultative methylotrophic bacterium isolated from rhizosphere of Eruca sativa

Pathological and mortality findings associated withAeromonas hydrophilafrom frog eggs in Al‐Ahsa region of Saudi Arabia

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