First Isolation and Identification of Neopestalotiopsis clavispora Causing Postharvest Rot of Rosa sterilis and Its Control with Methyl Jasmonate and Calcium Chloride

Shi, Tingfeng; Guo, Minwen

doi:10.3390/horticulturae8030190

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…Experiments found that MeJA in vitro can reduce the diameter of lesions, increase the activity of defense-related enzymes, and enhance the disease resistance of fruits [23,24]. It has been illustrated that the application of MeJA at appropriate concentrations in tomatoes [25], blueberries [26], citrus [27] and rosa sterilis fruits [28] is beneficial for preventing postharvest decay and improving fruit quality.…”

Section: Introductionmentioning

confidence: 99%

Combination of Selenium and Methyl Jasmonate Controls Postharvest Tomato Gray Mold by Damaging the Membrane System

Yuan

Xiè

et al. 2022

Horticulturae

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Tomato, which is regarded as an important worldwide crop, is susceptible to gray mold caused by Botrytis cinerea. Selenium and methyl jasmonate can act as antifungal agents against pathogenic infections. To clarify the effect of selenium and methyl jasmonate on the fungal pathogen, the spore germination and mycelial growth of B. cinerea were investigated in vitro using the growth rate method. Additionally, the electrical conductivity, soluble protein content, malondialdehyde content and oxalic acid secretion of B. cinerea mycelium were also determined to further explore the antifungal mechanism of selenium and methyl jasmonate. The results showed that selenium application significantly increased cell membrane permeability and malondialdehyde content, and methyl jasmonate treatment decreased the soluble protein content in mycelium of B. cinerea. Furthermore, supplementation of the medium with both selenium and methyl jasmonate effectively inhibited spore germination and colony growth of B. cinerea by compromising membrane integrity, and significantly reduced soluble protein content and the oxalic acid secretion of hypha. The resulting incidence of postharvest tomato gray mold with the combination of selenium and methyl jasmonate was 34.7%, which was approximately half of that of the control. To sum up, the combined use of selenium and methyl jasmonate inhibited the normal physiological activity and pathogenicity of B. cinerea, which suggests that selenium and methyl jasmonate have the potential for controlling gray mold disease caused by B. cinerea in postharvest fruits and vegetables. These findings may offer a promising and eco-friendly strategy to control gray mold disease in postharvest fruits and vegetables.

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Section: Introductionmentioning

confidence: 99%

Combination of Selenium and Methyl Jasmonate Controls Postharvest Tomato Gray Mold by Damaging the Membrane System

Yuan

Xiè

et al. 2022

Horticulturae

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“…In this study, the pathogenic fungus Neopestalotiopsis clavispora was identified in naturally deteriorated blueberries after harvesting in the Majiang district of Guizhou, and it was able to infest blueberries and induce blueberry fruit decline. Neopestalotiopsis clavispora has been associated with fruit rot in kiwi [34], strawberry [35], loquat [36], and Rosa sterilis [37]. The results presented indicate that pathogenic fungi may have a diverse host range and may constitute a substantial danger to the fruit and vegetable business.…”

Section: Discussionmentioning

confidence: 83%

Inhibitory Activity and Mechanism of Action with Thymol against the Blueberry Pathogenic Fungi Caused by Neopestalotiopsis clavispora

Ye,

Shuai,

Luo

et al. 2023

Horticulturae

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Decay caused by Neopestalotiopsis clavispora is an important postharvest disease of blueberries that seriously affects the commercial value of blueberry fruit. In this paper, we studied the inhibitory activity and mode of action of thymol against the pathogenic fungus of blueberries caused by Neopestalotiopsis clavispora. The results demonstrated that thymol administration could limit mycelial growth in vitro; the inhibitory effect was positively connected with thymol mass concentrations, and the minimal inhibitory concentration (MIC) was 100 mg/L. Further investigations revealed that MIC thymol treatment dramatically reduced the germination of pathogenic spores and led to an increase in the conductivity of the pathogen, leakage of contents, and a decrease in pH. Propidium iodide (PI) staining experiments demonstrated that MIC thymol caused damage to mycelial cell membranes. Additionally, MIC thymol treatment promoted mycelium malondialdehyde content accumulation, inhibited superoxide dismutase (SOD) and catalase (CAT) enzyme activities, decreased adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) content and energy charge levels, and the fluorescence intensity of mycelium caused by MIC thymol treatment was significantly increased by the 2,7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay. The results of this study indicate that thymol suppresses the proliferation of Neopestalotiopsis clavispora by compromising the integrity of their cell membranes, promoting the accumulation of cellular reactive oxygen species (ROS), and interfering with energy metabolism.

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“…These cells were distinguished by transparent terminal cells and dark inner cells, with appendages on the terminal cells resembling hooks, as depicted in Figure (3). This fungus is considered an important pathogen that affects both the roots and crowns [12].…”

Section: Resultsmentioning

confidence: 99%

Detection of Pathogenic Fungi Causing Root and Crown Rot Diseases in Strawberry Fragaria ananassa Duch

Abbas,

Abed

2023

IOP Conf. Ser.: Earth Environ. Sci.

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A study was conducted in the Department of Plant Protection, College of Agriculture, University of Anbar, aiming to identify the major fungi causing root and crown rot disease in strawberries through isolation and diagnosis using both morphological and molecular methods. the results of isolation and diagnosis from the affected plant parts revealed the prevalence of root and crown rot disease in all sampled areas exhibiting symptoms and signs of the disease. Seventeen isolates were obtained belonging to the Fusarium species, eight isolates belonged to the fungus Rhizoctonia solani, and seven isolates belonged to the spp. Neopestalotiapsis The fungal species were identified based on their taxonomic characteristics. DNA extraction was successfully performed on eleven isolated fungi from the roots and crowns of strawberry plants in different isolation areas. Partial diagnosis was conducted using PCR technique targeting the intergenic spacer region using primers (ITS1-ITS4) to obtain amplicons of 650 base pairs (bp) in size. The amplicons were then separated on 2% agarose gel, and the nucleotide base sequences of these isolates were determined and deposited in the gene bank.

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First Isolation and Identification of Neopestalotiopsis clavispora Causing Postharvest Rot of Rosa sterilis and Its Control with Methyl Jasmonate and Calcium Chloride

Cited by 5 publications

References 36 publications

Combination of Selenium and Methyl Jasmonate Controls Postharvest Tomato Gray Mold by Damaging the Membrane System

Combination of Selenium and Methyl Jasmonate Controls Postharvest Tomato Gray Mold by Damaging the Membrane System

Inhibitory Activity and Mechanism of Action with Thymol against the Blueberry Pathogenic Fungi Caused by Neopestalotiopsis clavispora

Detection of Pathogenic Fungi Causing Root and Crown Rot Diseases in Strawberry Fragaria ananassa Duch

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