First isolation of Acinetobacter johnsonii co-producing PER-2 and OXA-58 β-lactamases

Rodríguez, Carlos Hernán; Nastro, Marcela; Dabos, Laura; Barberis, Claudia; Vay, Carlos; Famiglietti, Ángela

doi:10.1016/j.diagmicrobio.2014.09.013

Cited by 15 publications

(12 citation statements)

References 11 publications

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“…Insert Table 2 Insert Fig.5 Discussion A. johnsonii strain M19 presented higher carbapenem resistance than that of most A. johnsonii strains [2,4,[28][29][30][31]40], Pseudomonas aeruginosa [41], Proteus mirabilis [42] or A. baumannii [43]. β-lactam antibiotic resistance of Acinetobacter is mainly due to the inactivation of β-lactams catalysed by four classes (A, B, C and D) of β-lactamases [9,13].…”

Section: Conjugative Plasmid Pfm-m19 Disseminates Bla Oxa-23 and Carbmentioning

confidence: 95%

The carbapenem resistance gene blaOXA-23 is disseminated by a conjugative plasmid containing the novel transposon Tn6681 in Acinetobacter johnsonii M19

Zong¹,

Zhong

et al. 2020

Preprint

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Background: Carbapenem resistant Acinetobacter species have caused great difficulties in clinical therapy in the worldwide. Here we describe an Acinetobacter johnsonii M19 with a novel blaOXA-23 containing transposon Tn6681 on the conjugative plasmid pFM-M19 and the ability to transfer carbapenem resistance.Methods: A. johnsonii M19 was isolated under selection with 8 mg/L meropenem from hospital sewage, and the minimum inhibitory concentrations (MICs) for the representative carbapenems imipenem, meropenem and ertapenem were determined. The genome of A. johnsonii M19 was sequenced by PacBio RS II and Illumina HiSeq 4000 platforms. A homologous model of OXA-23 was generated, and molecular docking models with imipenem, meropenem and ertapenem were constructed by Discovery Studio 2.0. Type IV secretion system and conjugation elements were identified by the Pathosystems Resource Integration Center (PATRIC) server and the oriTfinder. Mating experiments were performed to evaluate transfer of OXA-23 to Escherichia coli 25DN.Results: MICs of A. johnsonii M19 for imipenem, meropenem and ertapenem were 128 mg/L, 48 mg/L and 24 mg/L, respectively. Genome sequencing identified plasmid pFM-M19, which harbours the carbapenem resistance gene blaOXA-23 within the novel transposon Tn6681. Molecular docking analysis indicated that the elongated hydrophobic tunnel of OXA-23 provides a hydrophobic environment and that Lys-216, Thr-217, Met-221 and Arg-259 were the conserved amino acids bound to imipenem, meropenem and ertapenem. Furthermore, pFM-M19 could transfer blaOXA-23 to E. coli 25DN by conjugation, resulting in carbapenem-resistant transconjugants. Conclusions: Our investigation showed that A. johnsonii M19 is a source and disseminator of blaOXA-23 and carbapenem resistance. The ability to transfer blaOXA-23 to other species by the conjugative plasmid pFM-M19 raises the risk of spread of carbapenem resistance.

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Section: Conjugative Plasmid Pfm-m19 Disseminates Bla Oxa-23 and Carbmentioning

confidence: 95%

The carbapenem resistance gene blaOXA-23 is disseminated by a conjugative plasmid containing the novel transposon Tn6681 in Acinetobacter johnsonii M19

Zong¹,

Zhong

et al. 2020

Preprint

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“…Acinetobacter johnsonii was the second most abundant potential pathogen identified in the stormwater columns, which was on average 1.85-fold more abundant on columns than in the inoculum samples. A. johnsonii can be found in environmental samples [41], on the human skin [42], and associated with disease [43,44]. Other relatively abundant potentially pathogenic taxa were also classified as Stenotrophomonas or Acinetobacter.…”

Section: Dynamics Of Potential Pathogens and Denitrifying Bacteriamentioning

confidence: 99%

Dynamics and Functional Potential of Stormwater Microorganisms Colonizing Sand Filters

Fraser

Yue

Sakowski

et al. 2018

Water

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Stormwater management is increasingly relying on engineered infiltration systems (EIS) to reduce the volume and improve the quality of managed stormwater. Yet, EIS in the field will be colonized by a diverse array of environmental microorganisms that change the physiochemical properties of the EIS and provide a habitat for microorganisms with harmful or beneficial qualities. Understanding factors influencing the composition and stability of microbial communities could open up strategies for more efficient management of stormwater. Here, we analyzed the potential pathogenic and metabolic capabilities of stormwater microorganisms colonizing idealized EIS (i.e., sand columns) under laboratory conditions over time. The diversity of microbial communities was analyzed using 16S rRNA gene sequencing, and potential pathogens and denitrifying microbes were identified from taxonomic match to known species. Denitrification potential as determined by nosZ abundance was also assessed with quantitative polymerase chain reaction PCR. Our findings demonstrate that replicate microbial communities colonizing sand columns change in a similar way over time, distinct from control columns and the source community. Potential pathogens were initially more abundant on the columns than in the stormwater but returned to background levels by 24 days after inoculation. The conditions within sand columns select for potential denitrifying microorganisms, some of which were also potential pathogens. These results demonstrate that a diverse suite of stormwater microorganisms colonize sand filters, including a transient population of potential pathogens and denitrifiers. Manipulating the inoculating microbial community of EIS could prove an effective mechanism for changing both potential pathogens and denitrifying bacteria.

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“…Most of the reports were members of the Enterobacteriaceae family and also in Vibrio cholerae, Pseudomonas aeruginosa and Acinetobacter spp. [28,29,30].…”

Section: Analysis Of Blaper-2 Genetic Contextmentioning

confidence: 99%

Genetic analysis of a PER-2-producing Shewanella sp. strain harbouring a variety of mobile genetic elements and antibiotic resistance determinants

Almuzara¹,

Montaña

Lazzaro

et al. 2017

Journal of Global Antimicrobial Resistance

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The objective of this study was to investigate the molecular mechanisms explaining the multidrug-resistant (MDR) phenotype found in a novel clinical Shewanella sp. strain (Shew256) recovered from a diabetic patient. Whole-genome shotgun sequencing was performed using Illumina MiSeq-I and Nextera XT DNA library. De novo assembly was performed with SPAdes. RAST Server was used to predict the open-reading frames and the predictions were confirmed using BLAST. Further genomic analysis was carried out using average nucleotide identity (ANI), ACT (Artemis), OrthoMCL, ARG-ANNOT, ISfinder, PHAST, tRNAscan-SE, plasmidSPAdes, PlasmidFinder and MAUVE. PCR and plasmid extraction were also performed. Genomic analysis revealed a total of 456 predicted genes unique to Shew256 compared with other Shewanella genomes. Moreover, the presence of different resistance genes, including bla, was found. A complex class 1 integron containing the ISCR1 gene, disrupted by two putative transposase genes, was identified. Furthermore, other resistance genes, a transposon containing aph(3'), insertion sequences, phages and non-coding RNAs were also found. In conclusion, evidence of acquisition of resistance genes and mobile elements that could explain the MDR phenotype were observed. This Shewanella sp. represents a prime example of how antibiotic resistance determinants can be acquired by uncommon pathogens.

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First isolation of Acinetobacter johnsonii co-producing PER-2 and OXA-58 β-lactamases

Cited by 15 publications

References 11 publications

The carbapenem resistance gene blaOXA-23 is disseminated by a conjugative plasmid containing the novel transposon Tn6681 in Acinetobacter johnsonii M19

The carbapenem resistance gene blaOXA-23 is disseminated by a conjugative plasmid containing the novel transposon Tn6681 in Acinetobacter johnsonii M19

Dynamics and Functional Potential of Stormwater Microorganisms Colonizing Sand Filters

Genetic analysis of a PER-2-producing Shewanella sp. strain harbouring a variety of mobile genetic elements and antibiotic resistance determinants

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